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  1. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ... to make your own competent cells. If you need a high efficiency of transformation (for example, if you...However, you can skip this incubation step and go straight to plating if your transformed cells are being...

  2. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...sacB counter-selection) tools used in cloning. 8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1... Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest blogger Joachim...

  3. Editor's Choice, August 2016

    Type
    Blog Post
    ...experiences with CRISPR alternative, NgAgo. As you might expect with any nascent technology, researchers ...from Addgene Executive Director Joanne Kamens. I highly recommend taking a look at all of our posts as ...

  4. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...cancers are treated with drugs designed to hit them right where it hurts, the effects are often remarkable...that confer resistance to drugs,” in a rapid, straightforward, and scalable manner, Wood said. Thankfully...

  5. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ... we have a few unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style...

  6. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been...also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations than the first generation...

  9. London Calling - Addgene’s New Europe Office in London

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    Blog Post
    ...collaborators was extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really...really struggled to get those plasmids with the right information and I believe that a lot of European ...

  10. New Viral Vectors - Fall 2024

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    Blog Post
    ...monkeys it actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now...AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab...

  11. Antibodies 101: Affinity Tags

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    Blog Post
    ...proteins from E. coli is generally more straightforward and of a higher purity than mammalian purifications...

  15. Fluorescent Proteins 101: Fluorescent Biosensors

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    Blog Post
    ...signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters...transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation, and custom FRET...

  16. Tips for Improving Your Next Manuscript

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    Blog Post
    ...leaders in the field of microbiology with multiple high-profile publications. My classmates consisted of...scientific manuscript is not prose; it is to be straight to the point. 3. Engage the reader Scientists ...

  18. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... I settled on using glass slides. With just the right amount of agarose, I could pour a very thin gel ...surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t pour over the sides...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target...prefers a 'G' at the transcription start site to have high expression, so adding this G could help with expression...

  20. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...period. While there are many aspects to choosing the right labs (see my webinar (updated link from 2020) on...Mentoring" Don’t ignore warning signs If lab members, neighbors or alumni give you information about the PI that...
Showing: 741 - 760 of 980 results