We narrowed to 783 results for: post
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TypeBlog Post...affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series can help guide you...axonal transport (Murlidharan et al., 2014), and postsynaptic transmission (Oh et al., 2020). For example,...Others, like AAV1 and AAV9, can also transduce postsynaptic cells when used at very high titers (Zingg et...
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Sequencing Options for CRISPR Genotyping
TypeBlog Post...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University... -
CRISPR Guide
TypeGuide...screens, see our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...where they can scan for any signs of danger. In this post we’ll focus on how fluorescent proteins can and ... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog Post...surprisingly easy. It’s true, I CRISPRed. In this post, I’ll summarize the basic steps and analyses, and... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...for your experiment more complicated. We hope this post helps you in your search for the perfect way to ... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...What's more, depositing is free! We've written this post as a step-by-step guide to the online deposit process... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post... This post was contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...This post was contributed by guest blogger Pooran Dewari, a postdoc in Steve Pollard’s lab at the MRC... and happy genome editing! Pooran Dewari is a postdoc in Steve Pollard’s lab at the MRC Centre for Regenerative... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... knowing more? Check out our Mobile CRISPRi blog post. CRISPR activation (CRISPRa) While the activation... -
Technical Design of a Western Blot
TypeBlog Post...you’ll still have to lyse your proteins. For this post, we are assuming that you are running a denaturing... -
Using AAV for Neuronal Tracing
TypeBlog Post... combinations. Check out our retrograde AAV blog post. An additional desirable property for network visualization... -
Chemogenetics Guide
TypeGuide...: FLEx Vectors blog! Download our chemogenetics poster: References Alexander GM, Rogan SC, Abbas AI, Armbruster... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference at Boston ...fantastic city-wide effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka from Harvard...relieved because it finally feels to me like the postdocs are taking some steps to control their own future...feckless”. So what can be done? He charged the postdocs to “Grab ahold of your destinies” and also suggested...tractable” problems. We must better define the postdoc (and even the grad student) trainee experience ...Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, called her presentation “Disequilibrium...Disequilibrium, Disillusion and The Postdoc Dilemma” which pretty much said it all. She cited the oft ... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post...Interested in Addgene's Keystone Poster? We'll be sharing the poster with our blog readers and on social...meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant called..., & more via Addgene Addgene's addition to the poster session was a first for our repository, and I was...expect in terms of interest, but visitors to the poster were impressed to see how the numbers of plasmid... in the past 4 years. I kept busy for the whole poster session, talking to people from every conceivable...all the Addgenies who worked so hard to make this poster, offered suggestions and tips, and helped get me... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...data from postdoctoral programs in the Boston area that demonstrate that 85% of leaving postdocs pursue ...especially important for graduate students and postdocs to get a perspective on other types of science...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...recently it hadn’t been used to treat disease postnatally. Now, three papers published concurrently in ...shown CRISPR can treat a genetic disease in a postnatal mouse model, an important proof of concept for... that CRISPR and AAV can be used together for postnatal genome editing in mice. Could this approach work...Bhattacharyya S, Shelton JM, Bassel-Duby R, Olson EN. Postnatal genome editing partially restores dystrophin expression... -
Fueled by Coffee at #SfN14
TypeBlog Post...are thousands of posters being fueled by highly caffeinated grad students and postdocs, which accounts ...keep up with all the awesome talks, symposiums, poster sessions, exhibit booths, happy hour socials, and...