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We narrowed to 958 results for: Tes

Showing: 761 - 780 of 958 results

  2. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Given the variety of these enzymes and the unique sites they recognize, restriction digests have become ...cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector...Analyzer , allow you to identify which restriction sites are present in a given sequence. For a list of the...either PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms... restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus making...

  3. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...checked circle. Typically, a yellow check mark indicates that a particular parameter was automatically ...sequencing bases downstream of the cut. Summary ICE generates NGS-quality CRISPR editing analysis from Sanger...

  4. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...for labs and nonprofits, who tend to prioritize candidates that are excited about their work and mission... will be close to that of a professional email. Notes from real-life hiring managers In preparation for...

  5. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie, Center...assay. This may be due to competition for binding sites between the primers and the AAV genome repeats. ...

  6. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...of recruiters for identifying appropriate job candidates. If you do receive such a call, make sure to ...-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/15-surprising-rules-you-should-know-regarding-linkedin-etiquette...

  7. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. Blood 100:823–832...

  11. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...new window) Link to Video Making LB Agar Plates Create plates to culture bacteria in the lab Watch the...molecular biology, plasmid cloning, and titering and testing your viral preparations. Addgene...your plasmids. Virus Protocols for titering and testing your virus preparations. Antibodies Protocols for...and ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...of toxins before the second crossover step, they tested their expression under both rhamnose- and arabinose-inducible...selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. ...

  13. Five Popular Model Organisms

    Type
    Blog Post
    ...misfolding that leads to a build-up of protein aggregates termed “lewy bodies” in the brain. Yeast models...majority of C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...neurons in the neocortex of a living mouse. Image courtesy of Axel Nimmerjahn (Salk Institute for Biological...tags due to the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a silent, passenger...CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing you...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ... for the majority of documented phosphorylation sites. If knowledge of a candidate regulatory kinase is...that binds specifically to phosphate moieties and promotes a robust mobility shift in phosphorylated, but...

  18. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ...and Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs...

  19. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can ... cartoon, in which we are adding EcoRI and NotI sites to Your Gene of Interest (YGOI) for ligation into...amplified (usually 18-21bp). When selecting restriction sites, you should use a DNA analysis tool, such as Addgene...Analyzer , to allow you to identify which restriction sites are present in a given sequence. You want to choose...based cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1...
Showing: 761 - 780 of 958 results