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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 843 results for: ANG

Showing: 761 - 780 of 843 results

  1. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... Nonetheless, we recommend performing a buffer exchange before using the purified AAV in vivo . The iodixanol... Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration Note: Both steps could be completed...Pool clean fractions. Concentration and buffer exchange: Stocks of Poloxamer 188 Poloxamer 188, 10% solution...adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    ... very diverse family of viruses with broad host range and tissue tropism (Borkenhagen et al., 2019). AdVs...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...

  10. MXS Chaining

    Type
    Blog Post
    ...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic...

  13. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...enzyme, or enzymes that have compatible overhangs or no overhangs after digestion, you will need to use ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...
Showing: 761 - 780 of 843 results