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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 838 results for: ANG

Showing: 761 - 780 of 838 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...engineered AAV serotypes that further expands the range of AAV properties is available. While only natural...

  3. MXS Chaining

    Type
    Blog Post
    ...Central PMCID: PMC2574415. 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic...

  6. Colony Formation Titering Assay

    Type
    Protocol
    ...fresh media containing selection reagent Days 3–14: Change media as needed Days 14–18: Stain cells and count...empirically determined. Treat the target cells with a range of doses of antibiotic. Determine the minimum concentration...presence of selective reagent for 12 days with media exchanges every 3–4 days. Colonies were then stained with...presence of selective reagent for 12 days with media exchanges every 3–4 days. After 12 days of selection, no...

  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...

  19. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... Nonetheless, we recommend performing a buffer exchange before using the purified AAV in vivo . The iodixanol... Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration Note: Both steps could be completed...Pool clean fractions. Concentration and buffer exchange: Stocks of Poloxamer 188 Poloxamer 188, 10% solution...adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods...

  20. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...enzyme, or enzymes that have compatible overhangs or no overhangs after digestion, you will need to use ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...cloning. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...
Showing: 761 - 780 of 838 results