We narrowed to 1,013 results for: CAL

...sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of epigenetic ...from Addgene. For most of these constructs, catalytically dead modifiers are also available as controls...Resources on the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: How to Experiment...

...transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons...in a genome. But there is a type of mobile DNA, called a transposon, that’s a bit hyperactive and likes...

... a laughter program significantly decreased immunological markers of stress in adolescents. It also put...great use of your time even as that lab bench is calling, let me share my top five new favorite science ...

...understanding of how protein folding works and creating biological tools not found in nature. Dr. Baker’s team was...was not based on any existing protein structures, called Top7. Figure 1: Top7 receives the Nobel ...

...of an “electricity aggregation program” for all local households and businesses. Electricity aggregation... energy expenditure here at Addgene. These are critical systems that cannot be removed or substantially...

...preparations of several variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now...preparations of several variants of a new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so...

...wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind them you care!#thanks #...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea...

...with most reagents sent, and the most recent full calendar year When the website is launched, it will show...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...

...E6 regulatory element, which targets it to VIP cortical interneurons. Take a look to see if these new ...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse...

...September 13, 2018 This massive scale reagent sharing led us to calculate how much time we have saved researchers...

...:26 : Kwabena's plans for work in Ghana - Using local biomass "waste" to produce biofuels. 23:27 - 25:...foods. 44:15 - 46:34 : Kwabena's goal to build biological science opportunities in Ghana. 46:35 - end :...

...finishing up their time in the PhD Program in Biological and Biomedical Sciences at Harvard University. Ben and...

...acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI...scientists to continue to do excellent work in the biological sciences. Future awardees Now that this first...

...the antigen of interest. The immortalized cells, called hybridomas, are individually isolated and expanded...repositories provide standardized quality-control and technical support to the community. Thanks to Our Guest ...

...Hiroshima University. “However, there had been no systematically established way of making an all-in-one vector...Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening ...

...plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more ...E. coli, the Chong Zhang lab developed a genome-scale CRISPR inhibition pooled library (~60,000 gRNAs)...

...Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering Committee...A grant from the National Institute of General Medical Sciences (NIGMS) will support the development and...

... Support Specialists here at Addgene. If you’ve called our customer service line or sent an email to help...plasmids, store your plasmids or viral items, technical support, and more. ...

... by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR/Cas9... Times feature when he said, “All of this has basically happened in a year. It’s incredible.” Looking...

...the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later...libraries up to 200,000 individual plasmids and can be scaled according to the actual number. Required Quality...selection). This recombination, at a low rate, is not typically a problem as these sequences are not efficiently...200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly. Pro-Tip...cells) Less library DNA can be used but this dramatically increases the chances of individual plasmids...Cell Spreaders (VWR, 60828-680) 5 mL and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and ...normal growth is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare...