Hot Plasmids: Summer 2025
Type
Blog Post
...for visualizing cellular components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses, and...
We narrowed to 771 results for: Dos
-
-
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...Servellita V, Singh J, Miao X, Streithorst JA, Granados A, Sotomayor-Gonzalez A, Zorn K, Gopez A, Hsu ... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...CRISPRa is well-suited, including studying mRNA dosing effects, cellular reprogramming, and disease modeling... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...and Rebecca Ahrens-Nicklas administered the first dose of his personalized genetic medicine (Musunuru et... -
Hot Plasmids: Summer 2024
TypeBlog Post..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...commonly used vector cargo designs and administration doses. Don't forget to submit your validation data after... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...to remove salt, then suspend a freshly harvested dose of cells (preferably in log phase) in blocking solution... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...Choudhary A (2019) A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...purified antigen to demonstrate the expected antibody dose response....probe with an antibody against the target to show a dose response. Sharing speeds science. We believe that...standard curve . If you don’t see any color change or dose response, your antibody concentration may have been... the reaction is saturated and you don't see any dose response, your antibody concentration may have been... -
Western Blot
TypeProtocol...titrating your antibody to determine the optimal dose. Secondary antibodies must match the host species...titrating your antibody to determine the optimal dose. To ensure that your antibody is both functioning... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...purified antigen to demonstrate the expected antibody dose response....