... the “Analyze Sequence” button to pull up an interactive plasmid map powered by SnapGene and gain access...the static maps discussed above, but also have interactive functionalities. You can now hover over any feature...maps, the linear sequences hosted here are now interactive and have similar hover over features. As with...

...protein is based on the properties found in the literature or tabulated data that summarizes properties ...). However, the theoretical brightness ignores several important experimental conditions that are related...detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence...

...out of these interactions, ask yourself how every piece of feedback you receive generalizes.  If someone...skillset rather than a permanent fix. Treat every interaction in your work as an opportunity to practice communicating...

... how stretches of DNA on different chromosomes interact with each other. Chromatin regulation is complex...in the right direction! CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation... An Improved Method for Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR...

...talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...to an uninfected culture which results in less overall dilution and less stringent phage selection. ...

...closely associated and function as one unit: Gβγ. In general, GPCRs show a propensity to associate with certain...receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed ...Chemogenetics uses genetically engineered receptors to interact with small molecules to elicit a response in a...

...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques. If ...with only white colonies is very suspicious! Refrigerate your plates: Placing plates at 4C for few hours...care in making your plates: X-gal is light and temperature sensitive and needs to be added to media after...

...exposed to 500 nm violet light, the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids...

...the photostability measurements reported in the literature are performed in different ways. We describe ...intensity remains. The first issue is that, in several cases, the fluorescence intensity decay of a fluorophore... be identified by a head-to-head comparison of several fluorescent proteins of the same spectral class...

...implementing consensus recommendations, even when a federal mandate encourages them to do so. Immediately after...There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary and Beth Israel...salaries when all others in Boston (some of them literally across the street from these institutions) are...

...CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation that will permanently ablate...package cDNAs for genomic delivery and have many therapeutic advantages, such as tissue-specific targeting... to temporarily increase expression. There are several flavors of activators which can be used with this...

...from peer-reviewed publishing in a journal.  Accelerating Scientific Communication Preprints have been...Fig. 1: Stand aside, CERN - it's a new age of accelerating science! Photo courtesy of under a CC license...attendees to read and comment online! Want the general public (say, policymakers or community organizations...

... of this is gospel, but we think it's worth consideration. Everything will go wrong Whenever an order ... lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly left...easily as needed. In this case, the physical considerations of the laboratory make object composition a...

... randomly. Therefore, inserting loxP sites at deliberate locations in a DNA sequence allows for very specific...reciprocal crossover event. This event can have three general outcomes based on the location and orientation ...separate DNA molecules, a translocation event is generated at the loxP sites. How can I use Cre-lox? The ...

...and think of ways to relate the proposal and deliverables to the aims requested in the call. Step 2: ...technologies. Therefore, take time to review the literature and offer educated insight into what the desirable...each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended...

... the right choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not...question came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose...the office. Main takeaways from Meet an Addgenie Overall, the Meet an Addgenie project was one of the biggest...

...select the components they want for each module, generate the vector file, and quickly clone their vector... Step 4: Naming your assembled plasmid and generating the vector file on the Fragmid website. ...4, is to give the assembled plasmid a name and generate the vector file. Ta-da! Most (180/181) Fragmid...

... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...populations. All of these topics require additional considerations to resolve. Sequencing and assembly issues ...acquired from the MiSeq for assembly or with enough coverage over plasmid regions to confirm their presence...

...format that was stable for up to a year at room temperature (Pardee et al, 2014). As a demonstration of the...the project by initially designing and ordering several dozen versions of the toehold switches and amplification...Addgene As we report, we have embedded this next generation of toehold switch-based RNA sensors within an...

...in AT-rich genomes. Additionally, whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23...sticky end” transgene insertion mediated by Cpf1-generated overhangs. Exploring the efficiency of these two...