We narrowed to 856 results for: HA

1. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   ...light is of particular interest since blue light has limited applications in vivo. UV and blue light is...domain from A. sativa (Bubeck et al., 2018). LOV2 has a C-terminal helix that unfolds when stimulated by...

2. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   ...for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet, you’ll...

3. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   ... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...

4. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   ...all of the problem solving that I did in the lab has been invaluable in my more recent positions outside...

5. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   ...active measures in improving cross team communication has helped Addgene move from completing 2-3 large projects...

6. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   ...some logical pattern across the poster. This style has the advantage of being different and potentially ...

7. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   ... nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector...

8. Engaging with science and society at pgEd

   Type

   Blog Post

   ...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...

9. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   ...shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium, also known as the...

10. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...brain and muscle development. The Gronostajski lab has generated mice wherein it's possible to delete the...

11. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    ...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...

12. RNA Extraction Without A Kit

    Type

    Blog Post

    ...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and...

13. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ... exposure to increased intracellular calcium. It has been applied to mark active populations of neurons...

14. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    ...antibodies will stick permanently to a membrane that has been dried, no matter what stripping methods you ...

15. Antibodies 101: Conjugation

    Type

    Blog Post

    ...or capable of producing a visible signal, but it has a strong affinity for avidin and streptavidin, both...

16. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ...afterwards. To engineer these production pathways, MoClo has been used to assemble multigene constructs capable...

17. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    ...Other Resources The National Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. ...

18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ... This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, ...

19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    ... 2008) occasionally and certainly after a set-up has been changed (for instance after replacing a broken...

20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    ...Future of Research believe that, while the mandate has been removed, institutions should still go ahead ...