We narrowed to 852 results for: Met

1. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   ...available destination vectors for various delivery methods, along with compatible inserts. All destination...

2. Adapting Toehold Switches to Detect Zika Virus

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   ...the Biodesign Center for Molecular Design and Biomimetics and the School of Molecular Sciences at Arizona...

3. Hot Plasmids and Viral Preps - January 2021

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   ...to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting (FACS...

4. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ... with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First, they...

5. Plasmids 101: FLEx Vectors

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   Blog Post

   ...allowing for rescued expression of GeneR.   Sometimes also referred to as DiO (Double-floxed inverse...

6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...near your insertion site. Base editing, another method to create point mutations, also has a strict editing...

7. Hot Plasmids: Winter 2025

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   Blog Post

   ...possible with conventional electrophysiological methods, it has remained difficult with GEVIs. Not only...

8. Degrading DNA with Cascade-Cas3

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   Blog Post

   ... inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...

9. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ... 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions of organic...

10. Of Myc and Men

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    Blog Post

    ...have been continuously pursuing new and better methods to peer into the mysterious, microscopic world ...

11. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ... terminal repeat sequences. Human gene therapy methods, 23 1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W...

12. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation...

13. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...the protein's behavior in cells, in solution or something in between, you are using a tag with a serious...

14. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ... citation for you to use in your Materials and Methods and References sections when citing the use of ...

15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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    Blog Post

    ... to include your improvements, or maybe even a methods paper. The problem with this is that it may take...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...their plasmids are available from Addgene in the Methods & Materials or elsewhere in their papers. Maybe...

17. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...responsibilities. If you are in charge of completing many meticulous protocols or tasks, you’ve probably developed...

18. An Introduction to Adenovirus

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    Blog Post

    ...activate the immune system, and of the infection to sometimes result in cell death, has led to interest in using...

19. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post

    ... any other ancestry group. With the existing methodology, they estimate that the African ancestry-associated...

20. Lentiviral Vector Uses and Overview

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    Blog Post

    ... screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed...