We narrowed to 879 results for: NSI

1. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   .... These plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE...

2. CRISPR Challenges: Standardization and Homology Directed Repair

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   Blog Post

   ...Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently leads to high on-target editing and low off-...

3. In Vivo Biotinylation of Bacterial Fusion Proteins

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   Blog Post

   ...kinetics of the target ligand. Another important consideration is whether the affinity of an experimental protein-ligand...

4. The Strength of Story Telling

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   Blog Post

   ...project and contributions, rather than getting defensive or getting into ‘he said; she said’. We spoke ...

5. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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   Blog Post

   ...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...

6. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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   Blog Post

   ...directly conjugated primary antibodies can be expensive and may not provide enough signal to visualize...

7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   Blog Post

   ...Virus structure While all viruses have a genome inside a protein shell known as a capsid, some also have...

8. What Do I Do Now? Academic v. Non-Academic Career Decisions

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   Blog Post

   ....  Here are some questions and perspectives to consider. What do academic and non-academic careers in ...

9. 10 Great Guest Posts We're Thankful For

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   Blog Post

   ...AAVs function and indicates important things to consider if you’re getting started with them. CRISPR Pairing...

10. Plasmids 101: TOPO Cloning

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    Blog Post

    ...hydroxyl group! You may want to include extra extension time after the last cycle of PCR to make sure ...

11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...HDR, which could lower HDR efficiency. Higher sensitivity to off-targets More research is needed to compare...

12. Plasmid Cloning by PCR

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    Blog Post

    ...   The basic PCR primers for molecular cloning consist of: Leader Sequence: Extra base pairs on the ...

13. Addgene's Top 10 Blog Posts of 2015

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    Blog Post

    ...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...

14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post

    ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

15. New and Upcoming Viral Vectors - June 2019

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    Blog Post

    ...to the study of neuroscience. To this end, new biosensing AAV are always being developed and we are excited...

16. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ...fluorescent proteins under the control of promoters responsive to water or nutrient levels can help him determine...

17. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post

    ...pairs were easily visualized and results were consistent with copy number variation (CNV) analysis of ...

18. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    Blog Post

    ...physical location didn’t matter. I didn’t have to consider that aspect and I could plan more with regard ...

19. Plasmids 101: Gram Negative and Positive Bacteria

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    Blog Post

    ...into a specific bacterium, it can be important to consider the type of bacteria it is being used in, and ...

20. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post

    ...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in...