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We narrowed to 985 results for: nin

Showing: 761 - 780 of 985 results

  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...studies. These parameters are less important when designing a biosensor, as the distance between the two fluorophores...many measurement methods as feasible when first beginning to establish the FRET methodology for a given ...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...effective brightness reflects the concentration of BV-containing molecules in a cell and depends on the efficiency...Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent proteins: insights...

  4. Five Popular Model Organisms

    Type
    Blog Post
    ...before we even knew that DNA was genetic material (Jennings, 2011).   The fruit fly makes a great model organism...PMID: 23594743. PubMed Central PMCID: PMC3703927. Jennings, Barbara H. "Drosophila–a versatile model in biology...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...specific elution of biotinylated proteins. For help determining which biotinylation reagent to use check out ...Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules on surfaces.” Journal...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...autofluorescence’ include riboflavin in the medium or flavin containing proteins in mammalian cells. Generally, autofluorescence...  References 1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane...

  7. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...purify or overexpress proteins inappropriate in discerning their true roles. Work conducted by Kamada et...., Vidugiris, G., Machleidt, T., Robers, M. B., Benink, H. A., Eggers, C. T., Slater, M. R., Meisenheimer...

  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...light, this channel activates neuronal activity by opening and allowing positively charged ions to rapidly...You’ll need to test for these differences when designing your experiment.  Finding the right information...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    .... For example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased ...guided transposons in bacteria sets the stage for combining the super powers of CRISPR and transposons to ...

  14. Molecular Cloning Techniques

    Type
    Guide
    ...BioRender.com. TOPO Cloning Toposiomerase-based cloning, often called TOPO cloning or TA cloning, is a method... Gateway Recombination Cloning Gateway cloning is a recombination-based cloning method. During Gateway...Golden Gate cloning kits for your next cloning project. Read more in our Golden Gate Cloning blog post ...Gate cloning. Created with BioRender.com. Ligation Independent Cloning Ligation Independent Cloning (LIC...independent cloning Addgene Blogs Plasmids 101: Restriction Cloning Plasmids 101: Gateway Cloning Plasmids...about different molecular cloning techniques, focusing on seven common cloning methods. Educational...Molecular Biology Reference Molecular Cloning Techniques Molecular Cloning Techniques Restriction Enzyme Gateway...

  15. Modular Cloning Guide

    Type
    Guide
    ...Biology Reference Molecular Cloning Techniques MoClo Guide Modular Cloning (MoClo) Guide You may also ...Kits Blog: Plasmids 101 Modular Cloning Blog: Plasmids 101 Modular Cloning Applications and Kits Blog: Plasmids... Plasmids 101 Golden Gate Cloning Synthetic Biology Plasmids Modular Cloning (or MoClo) is a system for...assembly steps. Before beginning, scientists insert fragments of DNA containing basic parts (promoters...enzymes, and mechanisms. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome...RVD TALEN inserts in one cloning step rather than two. Golden GATEway Cloning Kit Other, Zebrafish Expression...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...

  17. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth...
Showing: 761 - 780 of 985 results