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  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...autofluorescence’ include riboflavin in the medium or flavin containing proteins in mammalian cells. Generally, autofluorescence...  References 1. Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post
    ...effective brightness reflects the concentration of BV-containing molecules in a cell and depends on the efficiency...Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent proteins: insights...

  3. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...light, this channel activates neuronal activity by opening and allowing positively charged ions to rapidly...You’ll need to test for these differences when designing your experiment.  Finding the right information...

  5. Plasmids 101: Using Transposons in the Lab

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    Blog Post
    .... For example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased ...guided transposons in bacteria sets the stage for combining the super powers of CRISPR and transposons to ...

  10. Plasmids 101 eBook 4th Edition

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    Blog Post
    ...from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth...

  14. 'Tis the Season to #DeckTheLab

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    Blog Post
    ...that will warm up the entire lab. We’ll choose 4 winning submissions and deliver the prizes at the start...

  15. The Twelve Days of Antibodies

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    Blog Post
    ...to me…  A recombinant Anti-DYKDDDDK!   🎶On the ninth day of Antibody, Addgene gave to me… A neuroscience...

  19. The 12 Days of CRISPR: 2021

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    Blog Post
    ...delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform...

  20. The 12 Days of CRISPR

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    Blog Post
    ... the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function using transcriptional...
Showing: 761 - 780 of 975 results