We narrowed to 838 results for: tat

1. Behind the scenes: Addgene’s new search engine and more

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   ...engine unique. Most importantly, with this new implementation, we laid the foundations for many improvements...

2. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   ...approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine which enzyme...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...

4. Early Career Researcher Toolbox: Social Media for Scientists

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   ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...

5. Antibodies 101: Isotypes

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   ...that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...

6. GPCRs: How Do They Work and How Do We Study Them?

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   ...create chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural...

7. A Quick Guide to a Career in Software Product Management

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   ...catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss which front-...

8. Building and Validating Recombinant Antibodies: A Case Study

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   ...). Figure from Andrews, et. al., Elife  A limitation of hybridoma generated mAbs is that if the frozen...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...discovered in 1946 by Joshua Lederberg and Edward Tatum when they observed genetic recombination between...

10. How to Make Friends and Meet People at a Scientific Conference

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    ...exhausted that you can’t get a lot from the presentations and programs. After the conference Follow ...

11. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ... to achieve success in this business. A good presentation can help win a business plan competition and...

12. Antibodies 101: Conjugation

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    ...Adler M, Wacker R (2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://...

13. To Each HIS Own

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    ... H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel...

14. Plasmids 101: The protein expression toolbox

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    ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

15. All in a Twist: dsRNA

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    ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

16. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are even going...

17. CRISPRainbow and Genome Visualization

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    ...fluorescently labeled Cas9’s come with a number of limitations. Since color is specified by PAM sequence, each...

18. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against...

19. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ...modeling. Its reversibility allows precise and quantitative analysis of epigenetic memory at nearly any ...

20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...further tested the base editors by correcting a mutation that causes Niemann-Pick disease type C, directly...