We narrowed to 847 results for: tat

1. Plasmids 101: Mammalian Vectors

   Type

   Blog Post

   ...Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS...

2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   ...by long terminal repeat (LTR) sequences, which facilitate integration of the transfer plasmid sequences...

3. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   ...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...

4. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   ... have been engineered to introduce frameshift mutations in genes of interest, turning expression off permanently...

5. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ...). Figure from Andrews, et. al., Elife  A limitation of hybridoma generated mAbs is that if the frozen...

6. Antibodies 101: Isotypes

   Type

   Blog Post

   ...that helps attack extracellular pathogens.  Transportation of antibodies to places they can’t reach on...

7. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   ...create chemogenetics receptors, GPCRs were first mutated via site-directed mutagenesis to bind non natural...

8. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   ... means recombinant proteins can aggregate or precipitate out as insoluble granules. We overcame this problem...

9. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ...engine unique. Most importantly, with this new implementation, we laid the foundations for many improvements...

10. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    ...ice. The Business and Finance Teams continue to facilitate the formation of partnerships to expand the service...

11. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    ...discovered in 1946 by Joshua Lederberg and Edward Tatum when they observed genetic recombination between...

12. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...

13. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    ...the three social media platforms, there’s some statistics on what percent of scientists and engineers regularly...

14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    ...approaches, knowledge of both the advantages and limitations of each Cas enzyme can help determine which enzyme...

15. How Addgene Creates Content

    Type

    Blog Post

    ... be surprised to learn that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies ...

16. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    ...catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss which front-...

17. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    ...exhausted that you can’t get a lot from the presentations and programs. After the conference Follow ...

18. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    ... to achieve success in this business. A good presentation can help win a business plan competition and...

19. To Each HIS Own

    Type

    Blog Post

    ... H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel...

20. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...