We narrowed to 641 results for: ATO

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...

...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt... Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral...

...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found...

...Find CRISPR plasmids to study the regulatory networks involved in retinal development....mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...

...Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used...illumination, making it a bimodal opto-chemogenetic actuator. For more detailed descriptions of chemogenetic...

...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...

...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR...

...Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus class I fusion protein upstream...TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein . Journal ...

...synaptic transmission with improved glutamate indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

...plasmid backbones into which you can clone your regulatory element or gene of interest to create a luciferase...transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection ...

...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression...., Wasserman, W. W., Simpson, E. M. (2010). A regulatory toolbox of MiniPromoters to drive selective expression...

...Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell ... . . Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron , 109...

... for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...feature afforded by coactivator complexes suggests that coactivators may be integrators of multiple cell ...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules...that coregulators belong to distinct complexes may explain how more than 300 different coactivator proteins...manner. Because coregulators exist as multiprotein complexes, a member of a single coactivator complex can... W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108, ...

...Choose a moderator with a backbone. It is nice to have a subject area expert, but the moderator is not ...LinkedIn profiles of all panelists to the moderator. The moderator and organizers should develop a list of...(lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from ...effortless to the audience, but the organizers and moderator should have a well-planned approach to making ... of 4-6 pre-event interview questions. The moderator (possibly with an organizer) should set up a 30 minute...to schedule. In the pre-event interviews, the moderator should be taking good notes and discovering interesting...opinions that each panelist can contribute. The moderator should identify the funny, provocative or interesting...

... the perfect voltage indicator and optogenetic actuator pair. Ideally, indicators should possess fast ...lab wished to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried out...kinetics. By combining both the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch...activation of specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(...62885 & 62886) Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche... which will ultimately co-localize the VP64 transactivator with a dCas9 that has been targeted to a very...tool for activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create a photoactivatable...

...Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani New viral...Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Gordon Fishell New viral prep pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...

...cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based research as...of the gene, Cas9 activators efficiently solve these problems.   Which Cas9 activator should I use? There...significant work on constructing Cas9 transcriptional activators. These constructs allow for the upregulation ...genome-wide studies One of the best uses for Cas9 activators is in genetic screening. gRNAs targeting every... using oligo library synthesis. Prior to Cas9 activators, similar tools were made using other DNA binding...to easily change the sequence targeted by the activator by simply providing a new gRNA rather than engineering...organism, have been used in a similar manner to Cas9 activators. However, these can be difficult to construct...

...Repressible: An activator protein is bound, turning transcription ON. When a repressor binds the activator protein...introduced the second generation QF2 activators. These activators solve the problem of broad QF expression-induced...protein, the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF....protein, the repressor protein can bind to the operator. The bound repressor then prevents transcription... repressor (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In... Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional...by Gossen and Bujard, both the promoter and transactivator components of the system have been engineered...

... of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the Church...method, called STARR-seq (self-transcribing active regulatory region sequencing) that tests enhancer sequences...promoters, RBS’, coding sequences (CDS), and terminators of varying strengths. These can be easily combined...both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform (CCP); the latter of which ...pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are...and context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto ...effectiveness of their library design, Moffat laboratory members conducted multiple screens in different...

...the activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator domain...control transcription. Two separate labs, Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach...directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the figure below...and CIB1 proteins heterodimerize and move the activator into position to activate gene transcription. ...fusion of full-length CRY2 and the transcriptional activator domain VP64. Using this system in HEK293T cells...NHEJ and HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable system to cleave...DSBs (non detectable). Like their prior work, the Sato lab also showed that the paCas9-2 system could be...