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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 112 results for: PIP;

Showing: 61 - 80 of 112 results

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...ongoing. Moving forward with ocular gene therapy The pipeline of AAV-based ocular gene therapies is impressive...

  6. DNA Purification Without a Kit

    Type
    Blog Post
    ...mammalian cells in 30 seconds or less without using a pipette.  Here’s how this method works: nucleic acids are...

  8. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Solubililty Assists in proper folding V5 GKPIPNPLLGLDST   1.4 Affinity and Purification Good for antibody-based...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... (SV5) (Randall et al., 1987). The epitope (GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et...

  11. Lab to Office Culture Shock

    Type
    Blog Post
    ...Finally, if you still find the idea of not holding a pipette again upsetting, there are ways to get back to ...

  13. March for Science

    Type
    Blog Post
    ... 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns Over Politicization...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...keep samples on ice at all times and use gentle pipetting instead of vortexing. Include proper controls ...

  16. Lentiviral Vector Guide

    Type
    Guide
    ... development of multiple genome-wide analysis pipelines to assess viral integration (see Kim et al. (2021... Hughes, S. H., & Wu, X. (2020). An analytical pipeline for identifying and mapping the integration sites...

  17. Pipetting Protocol

    Type
    Protocol
    ...the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette....you in selecting the correct pipette and pipette tip and using the pipette. Last Update: September 2022...Watch the video for tips on pipetting in the lab. Equipment Pipettes Pipette tips Waste container Containers...for pipetting Background Information Anatomy of a Pipette This front view photo of a P1000 pipette shows...which pushes the pipette tip off of the pipette. This side view photo of a P1000 pipette shows the volume... tip ejector down the pipette to remove the tip. Choosing a Pipette and Pipette Tips Although there are...idea of what pipette to use with it. The purpose of the pipette tip is so that the same pipette can be used...

  18. Molecular Biology Reference

    Type
    Guide
    ...DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK or DLYDDDDK Thrombin LVPRGS ...

  19. AAV ddPCR Titration

    Type
    Protocol
    ...single channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel...multichannel pipette 2–50 µL multichannel pipette 20–200 µL multichannel pipette Vortex, VWR, 10153-688 Mini...and pipette 5–10 times to mix. Dilute the virus as follows: Mix dilutions thoroughly, but pipette slowly...multichannel pipettes for the dilution series. For dilutions 1–5, use the 1–10 µL multichannel pipette set to...multichannel pipette set to 50 µL. For mixing between dilutions, use the 20–200 µL multichannel pipette set to...primers, and probe on ice before use. Wipe down all pipettes and surfaces with 10% bleach. Safety Warnings ...droplet generation are supplied with sufficient pipette tips and reagent reservoirs. Pro-Tip Prepare the...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ...single channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel...diluent, pipette 10 times, to remove virus from pipette tip. To mix the dilution, set the P200 pipette to 200...multichannel pipette 20–200 µL multichannel pipette Ice bucket 96-well freezer blocks Vortex, VWR, 10153-... 200 µL and pipette 10–20 times. Use a new pipette tip to transfer 200 µL of the first dilution into the...mixes and samples on ice before use. Wipe down all pipettes and surfaces with 10% bleach. Safety Warnings ...well before seeding. Mix each well with a 1 mL pipette 5–10 times. The final volume in the well is 1.5...6-well plates. Be sure to use a new aspirating pipette for each well as to not contaminate cell conditions...
Showing: 61 - 80 of 112 results