Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,100 results for: Ide

Showing: 121 - 160 of 1100 results

  1. Microbiology Resources

    Type
    Collection
    ...Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms... our deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe...community that aren't in the repository? Help us widen our resources for the community by depositing your...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology....experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...to be associated with the disease and implicate a wide range of cellular processes. The vast majority of...affects movement. More than ten million people worldwide are living with PD, mostly people over the age...associated with Alzheimer's disease, through large genome-wide association studies (GWAS) or other large scale ...modification and target species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy...A multicolor atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan...animal models, purified protein, and more. Plasmids provided by the foundation can be found on our Michael ...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well...plasmid. The Mendenhall and Myers labs have also provided their protocol for homology arm cloning: Mendenhall...single plasmid targeting vector that encodes both a guide RNA transcript and a repair template for an individual...epitope tags. The plasmids in the following articles provide PCR templates for amplification of the tag (eg ...Nucleic Acids Res. 2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging...

  4. Antibodies

    Type
    Collection
    ...control methods. Learn More Guide New to using antibodies? In our Antibody Guide, we define key terms, summarize...experts in the field to develop new antibodies and provide experimental data for recommended applications....Interested in our production process? At Addgene, we provide transparency in our production process ...techniques, and explain common applications. View Guide Need Some Help? Browse some of our other resources...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, inhibits...parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and... CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag ...

  6. AAVED

    Type
    Collection
    ... meeting will be a co-author of the guide. We hope that this guide will improve technology adoption and...the meeting will be a publicly shared practical guide for all scientists in the field and every attendee...lab (main issues/factors that researchers should consider when planning experiments). What resources might...

  7. p53 Pathway

    Type
    Collection
    ...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, ... PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger...

  8. Viral Production

    Type
    Collection
    ...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than...to the cleared viral preparation pool. Proviral Identity Confirmation and Sterility All of our lentiviral...

  9. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...the time of shipment. If any discrepancies are identified during this analysis, you will be notified by... Addgene performs QC measures which verify the identity of the viral vector, but not the function. Upon... years of the order being shipped, Addgene can provide the QC validation data performed on the material...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ... to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an ... also have the unique potential to continuously divide while maintaining an undifferentiated state. Stem...

  11. mTOR Pathway

    Type
    Collection
    ...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic...

  12. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...more attractive candidate for that ideal job. How can you identify your transferable skills? PLoS One ...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...when a new venture presents itself even outside of work, consider challenging yourself, take the opportunity...All while still paying bills and having a life outside my job. The same can be said about teamwork, communication...transferable skills so important? It is important to identify which transferable skills you possess because ... and making connections, avoid falling into an identity trap where you assume your technical skills define...wants to hear a specific time that you thought outside the box to solve a problem. When they ask about...

  13. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...high fidelity ligase—and when is an alternative ligase a better choice? And what is ligase fidelity anyway...are "higher fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase...annealed complementary regions. Why use a high fidelity ligase? Numerous applications have been developed...developed to take advantage of the high fidelity of Taq and other thermostable DNA ligases. These applications...applications detect specific nucleotide sequences with high specificity and quantitative accuracy and even include... include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase Detection...PCR amplified fragment) and treated with a high fidelity thermostable DNA ligase. If the target sequence...

  14. Zinc Finger Consortium Reagents

    Type
    Collection
    ...additional pages describing these reagents are provided below: Zinc Finger Genome Engineering Reagents...

  15. NETRF

    Type
    Collection
    ...Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal...

  16. MAPK Plasmids

    Type
    Collection
    ...also like... Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein...

  17. AAV Viral Preps

    Type
    Collection
    ...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  18. Plasmid Collections

    Type
    Collection
    ...together based on type or application. Browse our guides to find the plasmids and science information you...

  19. Synthetic Biology - Overview

    Type
    Collection
    ... community. To deposit, view our SynBio Deposit Guide or start our online deposit process . Browse SynBio...

  21. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Other Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416...myc HIS SMN1 His, Myc CMV Spinal muscular atrophy Gideon Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP T7 Alzheimer's...pFBD-BirA_LRRK2:2141-2527 LRRK2 His, Biotin acceptor peptide tag polH Parkinson's Cheryl Arrowsmith 210986 pHTN-HaloTag_C9orf72... found to match all requested criteria. Please consider using fewer filtering options. Browse all Neurodegeneration...

  22. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ... contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene...within the Addgene catalog. These IDs allow you to unmistakably identify a plasmid available through Addgene...VQR-Blast, which has the Addgene ID #87155. The resolvable compact identifier format for lenti-Cas9-VQR is...will be assigned an Addgene ID #, RRID, and resolvable compact identifier. Addgene works hard to ensure...including plasmids. All plasmids at Addgene have unique ID numbers associated with them, which serve as unique...about each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up...the “Articles Citing this Plasmid” section. Having IDs linked to these metadata and publications help to...

  23. CRISPR Guide Update

    Type
    Blog Post
    ...is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how to use...incredible new CRISPR technologies developed and widely adopted in the past few years, as well as refreshing...refreshing the existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime... PRIME editing Please check out the updated guide for a great introduction to what CRISPR technologies...

  24. A Scientist's Guide to Ebola

    Type
    Blog Post
    ... viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows that the Ebola...week within two months. That said, Ebola is not considered a “terribly infectious disease” – infection occurs...via body fluids not through the air and that’s considered unlikely to change – but the illness has been...thought we’d do a little digging and assemble a rough guide to the science of Ebola and related resources. Ebola...

  25. New Coomassie Protocol Video

    Type
    Blog Post
    ....    If this video is helpful, you can find our complete collection of protocol videos on the Addgene...antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

  26. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ...then, in having a guide that curates this information. And so I created the Codon Guide to Synthetic Biology...The video channels are also underrated and I recommend checking them out, especially since videos on synthetic...knowledge but little biology experience.) The Codon Guide to Synthetic Biology One of the best ways to learn...begin their journey into synthetic biology. The guide is a curated list of research papers, blogs, free...send me suggestions. The most useful parts of the Guide are probably the papers and textbooks. I’ve selected...to find. I hope that many people will find this guide useful as they start their synthetic biology journey...

  27. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ... and adenoviruses in this easy-to-use guide. Print out the guide and use it for quick reference when you're...information on viral vectors? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service...pages. Click on the Image Above to Download the Guide ...

  28. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...alone. So, if you consider yourself part of the scientific community, get used to the idea of working on ...our kit process substantially and have plenty of ideas for further improvements. Types of teams There ...the first few meetings and we’ve volunteered our ideas, skills, and time to the cause ever since. Unlike... necessarily need a diverse group of people to provide that base. Sometimes there’s just too much work...ever taken has declared me an introvert, and I identify as one.  But some time around 10th grade, I snapped... a happier person. What does a good team need? Besides that all-important sense of purpose, there are ...multiple people: someone to coordinate, someone to decide, someone to delegate, etc. If you find that there...

  29. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New ...Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills Guide: Identifying...are still struggling with idea generation, try this thought exercise: Consider the issue as if there were...faster that way— but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box...most prominent idea, and instead building an environment where everyone has their ideas heard.  So how...in which the goal is to generate unique ideas. Recognize ideas can come any time. Meetings shouldn’t be...to encourage the sharing of ideas, not to give people more work. Identify your enemies In Felicia Day...

  30. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...three or four guides directed towards that gene as there can be a large difference in guide potency. Thus...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to a ...scientific world by storm. While Cas9 has been most widely used to create specific edits in DNA, there has...required for Cas9-based activation. CRISPRa for genome-wide studies One of the best uses for Cas9 activators...  Which Cas9 activator should I use? There are a wide variety of activators you can use for your experiments...transcription. That being said, we generally pick guides by putting the promoter of the gene into a gRNA...(2015) WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system. Genome Biol 16:...

  31. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...dumpsters outside the open windows in the middle of your talk. Technical difficulties turn your slide deck ...interest incorrectly on slides 8-16. Your notes disappear. The wrong version of your slide deck gets uploaded...hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents above–and...Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict Resolution Transferable...stand up in front of an audience and convey your ideas clearly and strategically is powerful in almost ... allows you to share and build support for your ideas, invite others into decision making processes, and...help me achieve my purpose? Should I go over this slide again? Say, 'Oh, I forgot to mention X, so let's...

  32. New Western Blot Protocol Video!

    Type
    Blog Post
    ...first-timers. That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi...protocols on our Protocol page, and find our protocol videos on our YouTube page.     ...

  33. Important Considerations When Using AAVs

    Type
    Blog Post
    ...investigator who is using an in vivo animal model should consider the possible elicited immune response from viral...AAV. While these benefits make AAVs useful for a wide variety of experiments, care still needs to be taken...particular AAVs to use for your research. Below I’ll provide some tips that will help you think about how to...from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue/what cell type? In the CNS,...distance traveled, etc.) (4). This has also been identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory... many experiments, a group of researchers has identified this feature of AAVs as a new tool for neuroanatomical...miRNAs), short hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases, and many others...

  34. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...vectors Although retroviruses are still in use, considerable efforts have been devoted to develop efficient... Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector system is the ...manipulated with care. See the NIH’s biosafety guidelines for more information and always be sure to consult...another. For experimental consistency it’s a good idea to purify and titer your virus. Virus purification.... Don’t hesitate to check our lentiviral vector guide to get more information and to get access to additional...Order Ready-to-Use Lentivirus Browse Our Lentivirus Guide Pages  ...

  35. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...Today in our transferable skills guide: Collaborating with others outside your organization also known as...communicate outside my scientific expertise. Organizing a career fair for early-career scientists provided a crash...Interestingly, the ability to work with others outside one’s organization was found in the aforementioned...important in a non-research context, especially when considering small to mid-sized companies and organizations... Addgene as a nonprofit organization is very considerate and thoughtful about its limited resources. Rather... curve for collaborating and it is always a good idea to start close to your comfort zone and slowly expand...Collaboration opportunities within an organization but outside your comfort zone As part of my position at Addgene...

  36. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ... The Addgene Videos Page 5 Science Rap Videos and Their Creator: Tom McFadden Using Video to Share Your... we’ve gained along the way to ourselves so we decided to share some of our tips in our new series: Addgene...Have some suggestions for future tips?  Send your ideas to [email protected].  Additional Resources on the...Additional Resources on Addgene.org Browse our Video Library Check out Our Protocol Pages Find Molecular...

  37. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... that one on one discussion can be confidential. Finally, provide timely feedback. We often associate...need some help. Today in our transferable skills guide: managing a team. You’ve just been promoted, congratulations...advance as much as possible so I could more easily identify what to delegate and to whom (make sure that person...expectations, answer any questions, and (most importantly) decide on a timeline. My experience is that tasks often...of X’s work for a couple of weeks now”. I had no idea this was happening even though I met with X on a...when having a mentor - someone more experienced to guide you - is valuable. Protect your team Make you team...impact your work and your life. It’s up to you to decide which one you want to be!  References Becoming...

  38. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...need some help. Today in our transferable skills guide: Time Mangement for Scientists. Back when I used...my own experiments in graduate school, I had to divide my time between lab work and practicing my SciComm... but if you ever find yourself in need of a few ideas, I hope you’ll find this post useful. After a bit...which tasks to actually do, you should err on the side of things that have the most utility; i.e. those...according to my priorities, I have a pretty good idea about the specific things I’ll be doing through ... it’s enticements are far too distracting. (As a side note, I’d recommend the book, “Bored and Brilliant...

  39. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...backgrounds. Sometimes this can lead to wildly different ideas of how to approach a problem, but it is these very...Addgene. Another advantage of Scrum is that it provides transparency to everyone involved about the status...serve Addgene and ourselves. We are also able to consider different technical approaches that may come up...from the user's perspective which in turn helps us decide how to best approach it in our code.  Sometimes...our knowledge, and love to collaborate, which, incidentally, are the same principles for open science. I...

  40. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...comprehensive guide to Conflict Resolution. There are countless classes and resources that can provide details...ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork  ...” in quotations here because I would not have considered myself neutral in any of their disagreements....open new career opportunities for you, if you can identify them in yourself and learn how to sell them. Types...decision making, and stalled projects. You want to identify and address these types of harmful conflict early...other parties, the first step I always take is to identify the real source of the conflict. It can be easy...important to retain the stories and examples that have guided your resolution processes in the past. Types of...
Showing: 121 - 160 of 1100 results