We narrowed to 163 results for: NAP

1. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   ...The images in this blog post were created using Snapgene and Geneious software. References and resources...

2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...Drosophila cell lines are extremely effective. Their Snapdragon tool helps you select primers to synthesize RNA...

3. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...

4. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources on Addgene.org...

5. Which Fluorescent Protein Should I Use?

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   Blog Post

   ...e.g. yeast cytosol, which is slightly acidic, or synaptic vesicles). Some FPs have different ex/em spectra...

6. Summer SciComm Series: Modes of Communication

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   Blog Post

   ... or burn yourself out trying to slog through unappealing work in your precious free time.  And don’t be...

7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   Blog Post

   ...this concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems...

8. CRISPR Antimicrobials

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   Blog Post

   ...virulent or antibiotic resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria...

9. Isolating Genomic Regions of Interest with the CRISPR System

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   Blog Post

   ...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

10. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...job requires day-to-day, and you’ll get a great snapshot of the skills that you can bring into pretty much...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...C , Xu M, Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, ...

12. Plasmids 101: Protein tags

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    Blog Post

    ...HHHHHHAGKA GEGEIPAPLA GTVSKILVKE GDTVKAGQTV LVLEAMKMET EINAPTDGKV EKVLVKERDA VQGGQGLIKI GVHHHHHH  9 Combo Consists...

13. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ... e-nnovation Life Sciences 2020 India Sinapse Biotecnologia 2022 Brazil   In some cases...

14. Plasmids 101: Codon usage bias

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    Blog Post

    ...codons, the ribosome may therefore stall at inappropriate locations or fail to make it through the entire...

15. Plasmids 101: Gateway Cloning

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    Blog Post

    ..., etc). This video demonstrates how to use the Snapgene program to design Gateway plasmids.    ...

16. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post

    ...detect possible errors in assumptions, use of inappropriate statistical methods, omission of some data, ...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

18. March for Science

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    Blog Post

    ...cause. The references provided also only provide a snapshot into the opinions or research on each topic. Resources...

19. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

    ...PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome segregation in human cells harboring...

20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post

    ...detail how neurons form circuits and where they synapse. View the overview schematic at the bottom of this...

21. Plasmids 101: Dimers and Multimers

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    Blog Post

    ... of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing affordability...

22. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post

    ...tendency – mean, median, and mode give you a summary snapshot without telling you anything about outliers or...

23. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    ...variable) Sequence-level data Low-throughput, inappropriate for heterogenous populations, low sensitivity...

24. Transferable Skills Guide: Teamwork

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    Blog Post

    ...identify as one.  But some time around 10th grade, I snapped out of the phase of my life that had me listening...

25. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...to photo-stimulate mammalian neurons and alter synaptic events: a new technology was born, and the term...

26. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post

    ...examples of these self-labeling proteins would be SNAP-tag® and HaloTag®. These consist of a genetically...

27. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...Williams, L.A., B.N. Davis-Dusenbery, and K.C. Eggan, SnapShot: directed differentiation of pluripotent stem ...

28. CRISPR Guide

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    Guide

    ...Stan, T., Tysinger, E., Hong, L., Yudistyra, V., Ponnapati, M. R., Jacobson, J. M., Church, G. M., Jakimo...Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade...

29. Depositor Collections

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    Collection

    ...Collection Synthetic Biology Voigt Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids...

30. AAV Viral Preps

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    ...Affinity Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype...

31. Chemogenetics AAV Preps

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    Collection

    ...hM4D(Gi) DREADD KOR DREADD PSAM4 GlyR Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element...

32. Control AAV Preps

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    ...CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other...

33. Ras Pathway

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    ...v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho...

34. CRISPR Guide

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    Collection

    ...Stan, T., Tysinger, E., Hong, L., Yudistyra, V., Ponnapati, M. R., Jacobson, J. M., Church, G. M., Jakimo...Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade...

35. Brain Armamentarium

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    Collection

    ...Ting Viviana Gradinaru 163489-AAV1 AiP1051-pAAV-Synapsin promoter-oNigri-WPRE-hGHpA Direct-expressing oNigri...

36. Neurodegeneration Research Collection

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    Collection

    ...activation kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023...

37. Cre-Lox and Other Site-Specific Recombinases

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    ...Plasmid Collection Optogenetics Plasmid Collection Monosynaptic Neuronal Tracing CRISPR Plasmids and Resources...

38. Retrograde AAV viral preps

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    Collection

    ... CAG GCaMP8s Calcium sensor Looger 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate...

39. Luciferase Plasmid Collection

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    Collection

    ...Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C...

40. Tetracycline Inducible Expression

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    Collection

    ...-syn-FLEX-splitTVA-EGFP-tTA Helper virus for monosynaptic tracing. To be coinjected with pAAV-TREtight-mTagBFP2...