...Fragmid system was used to assess how different lentiviral plasmid backbone elements impact viral titer ...expand the Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems...

...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...that Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources...

...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin...lines generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37...control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...

...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

...Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base ...Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871...

...2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the ...

...Our CRISPR Guide Pages Find Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership...

...cell types. A key feature of this system is a lentiviral gRNA expression vector (pRDA_526) that (1) enables...

...Addgene would be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - ...

...CRISPR-Cas9: Tips for Optimizing sgRNA Activity Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

... and requests are still pouring in! While our lentiviral service is going strong, the AAV service has ...

...et al., 2018). From this data, they generated lentiviral constructs using the High-Throughput Mutagenesis...

...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown,...

...CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis...

...comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the...

...unknown protein in macrophages/monocytes using lentiviral expression vectors. I also used plasmids made...

...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ...

...specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9...

...after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that...

...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...

...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...

... 10 guide RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker...

...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...

...has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...

...proven useful to the study of SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2...

...vector background, so you can package them into lentiviral vectors if you need to. Additionally, they have...

...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...

...Development of a novel mouse glioma model using lentiviral vectors." Nature medicine 15.1 (2009): 110-116...

...ready-to-use adeno associated virus (AAV) and lentivirus are now available from Addgene. While super useful...

...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...

...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

...CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ...

...high-throughput manner. They designed the pXPR_053 lentiviral expression vector, which contains an sgRNA insert...

...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing...

...? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

...Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene...

...in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene, the lab will express these...

...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in...

...technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery tools such...