We narrowed to 277 results for: mel

1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   Blog Post

   ...organs in whole animal models like Drosophila melanogaster, obtaining enough material for a ChIP-seq experiment...

2. Fluorescent Proteins 101: Fluorescent Biosensors

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   ...another common class of biosensors. Blue flame “cameleon” calcium sensors created by Roger Tsien’s lab ...

3. Tips for Improving Your Next Manuscript

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   Blog Post

   ...Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming a successful writer...

4. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   ... :D) engineering E. coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend much...

5. Management for Scientists: Giving Feedback

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   ...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...

6. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...circuitry and synthetic biology, and I found myself extremely grateful to my colleague Jason for choosing Simon...

7. Advice for Choosing a Research Project

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   ...productivity and direction of the project? What is your timeline? A typical research project can take several years...

8. The Stingy Scientist: How the Baby Gel Box Was Born

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   ...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...

9. Choosing a Good Mentor for Scientists

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   ... Consider reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying...

10. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    ...unstable ground around these hot springs make them extremely dangerous, we used telescoping poles to collect...

11. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    ... Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990...

12. What the HEK?

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    ...viral therapies. Protein production  HEKs are extremely easy to transfect in bulk and produce recombinant...

13. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post

    ... Melina Fan...

14. Meet the New Addgene Board Members!

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    ...delivery. In 2018, he interviewed Addgene co-founder Melina Fan and former Associate Director of Viral Vectors...

15. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    ... Melina Fan...

16. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post

    ...industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is concentrated...

17. Plasmids 101: TOPO Cloning

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    Blog Post

    ... at a low concentration or you are cloning an extremely large insert. Since the standard ligation reaction...

18. Plasmid Cloning by PCR

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    ...should select an annealing temperature based on the melting temperature (Tm) of the portion of the primer that...

19. Addgene's Top 10 Blog Posts of 2015

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    ...you’ve made the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation...

20. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    ...made CRISPR/Cas9 so successful in this species, namely, the robust HDR mentioned above, an accessible ...

21. K. phaffii: Rising to the Occasion

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    Blog Post

    ...Affordable to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to...

22. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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    ...Credit all contributions.   Our volunteers are extremely diverse. Some of them are undergraduates or PhD...

23. Networking Follow-up: I Met a Person. Now What?

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    ...where you met and what you discussed. Do this in a timely manner (within a few days or a week) so they can...

24. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    ...consensus guidelines, but there was never a specific timeline for this plan—or any action to follow it. And ...

25. Trading Spaces - Visiting Scientist Edition

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    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...

26. Addgene Depositors Get More Citations

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    ... and Neil Thompson. Special thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile...

27. How to Lead a Great Meeting

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    ...to an hour or less and are able to provide more timely feedback on each other’s projects. For all-company...

28. Management for Scientists: Delegating is Key

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    ...assignment to discuss goals, expected outcomes, timelines and deadlines. Agree on a way to review project...

29. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...green fluorescent protein that is supposed to be extremely bright. In S. cerevisiae, we've tested a number...

30. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    ...when managers fail to set expectations or provide timely feedback, leading to unproductive dynamics. I experienced...

31. GCE4All: Making Genetic Code Expansion Accessible

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    ...sensitive balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively...

32. Antibodies 101: ChIP

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    ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...

33. Sweating the Small Stuff: Details in the Lab

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    ...when she particularly needed to focus, which was extremely effective. Others ran experiments at odd hours...

34. GPCRs: How Do They Work and How Do We Study Them?

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    ...Alyssa Cecchetelli and Andrew Hempstead. What do smell, taste and sight have in common, besides being one...

35. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...in real time. Addgene depositor Professor Max Krummel from UCSF showed some impressive videos of these...

36. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ...or neutral, as compared to wild type, would be extremely helpful as a complementary approach. Also, in ...

37. Addgene’s Viral Service - Why Virus? Why Now?

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    ...” Under the leadership of Addgene’s co-founder, Melina Fan, we created a new team to push this project...

38. CRISPR 101: Non-Homologous End Joining

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    ...repair by NHEJ are typically small (1-10 bp) but extremely heterogeneous. There is consequently about a two-thirds...

39. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ...screening for genes that, when turned on, make melanoma cells resistant to treatment with a BRAF inhibitor...

40. How to Make Friends and Meet People at a Scientific Conference

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    ...level. Right away you have something in common. Namely, the scientific question you are interested in ...