We narrowed to 389 results for: ARIA
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TypeBlog Post...Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique ...
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New and Upcoming Viral Vectors - December 2019
TypeBlog Post... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog Post... their gene sequence may have slight or large variations depending on how closely related these species...percentage were not good predictors. There is not much variation in GC content between many organisms and DNA synthesis... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and... -
Hot Plasmids - January 2023
TypeBlog Post...Facility Isotype IgG2a Differences: Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized... and results were consistent with copy number variation (CNV) analysis of the U2OS cells. Figure... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...comparisons between lines expressing different variants of YFG because the transgenes are found in the...that are anywhere from light yellow to red. The variation in color depends on how transcriptionally active... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection... -
Viral Vectors 101: Viral Applications
TypeBlog Post..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...Japanese firefly (Luciola cruciata), and more. These variant proteins offer different kinetics, substrate requirements...measurements of luminescence from live cells. Luciferase variants with incompatible substrates can also allow multiplexing... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...Different setting, different population, new genetic variation, or different handling can all be important. ...certain that some important, previously unknown variable actually makes a big difference for the robustness... -
Plasmids 101: Screens vs. Selections
TypeBlog Post...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment. For example, I used... -
Plasmids 101: Inducible Promoters
TypeBlog Post...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post... available. We’ve also had some requests for variations on the plasmids we’ve developed. We’re working...be doing something similar. There may be other variants of this that are better for some experiments than... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...homodimerization is characterized by an affinity of 110 µM (Zacharias et al, 2002), meaning that at this concentration... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...regenerating tissues without using genome engineering. Planarian worms cut into three segments “remember” which...protein-mediated cues control polarity during planarian regeneration." Developmental biology 339.1 (2010... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...for oxidizing environments to photoconvertible variants, it seems like GFP is always learning new things...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is... -
RNA Extraction Without A Kit
TypeBlog Post...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise... -
Plasmids 101: Cre-lox
TypeBlog Post...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ... -
Michael J Fox Foundation (MJFF) Plasmid Collection
TypeCollection...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome editing...out this post. Learn how to Choose the Best Cas9 Variant for Your Next Experiment Read more about Optogenetic... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post... deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...noticeable influence on the outcome and cause variability in the testing group. For example, if an experimenter...distracted) from the ones tested at the beginning. Variability source Standardization parameters Animal Strain... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed. Several variations of this technology can be used to customize the... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...2010 Yuhai Cui Red mScarlet Split-wrmScarlet variant of mScarlet Split-wrmScarlet and split-sfGFP: tools...-10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1-10) Improved split fluorescent proteins... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP....14581454. PubMed Central PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified ... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About Designing...Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...stick out to form the “arms” holding the highly variable regions that bind to specific antigens. In contrast... Cloning System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...human disease. However, the rate of transfer was variable. They measured transfer rates by comparing the... of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to... -
Pooled Library Amplifications
TypeBlog Post...unique plasmids does your library contain?), the variance of size of plasmids in your library (Are the inserts... Library Type Name Number of Plasmids Variable Region Size DNA per Amplification Amplification... -
Transferable Skills: Negotiation
TypeBlog Post...covered separately in a future post. Resources Maria recommends: Getting to Yes: Negotiating Agreement... Maria Genco... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain... -
Getting to Know Expansion Microscopy
TypeBlog Post...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post... where absorbance readings are inherently more variable. If you do need to work with dilute samples: ...is commonly used for elution, it can give more variable absorbance readings. A Tris-buffered solution ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...parameter. We approximated the data likelihood with multivariate Gauss-Hermite quadrature, since the analytical...intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects of weekends... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you... -
CRISPR Plasmids - Parasites
TypeCollection...disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective brightness... -
Plasmid Collections
TypeCollection...Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like factors... -
Optogenetics Plasmid Collection
TypeCollection...light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data... -
Hot Plasmids: Fall 2024
TypeBlog Post...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...