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We narrowed to 389 results for: ARIA

Showing: 151 - 200 of 389 results

  2. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Facility Isotype IgG2a Differences:  Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for...

  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...typically have a wide range of input quantities. This variation is problematic for conventional NGS library prep...range (1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and...

  6. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized... and results were consistent with copy number variation (CNV) analysis of the U2OS cells.   Figure...

  7. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...Japanese firefly (Luciola cruciata), and more. These variant proteins offer different kinetics, substrate requirements...measurements of luminescence from live cells. Luciferase variants with incompatible substrates can also allow multiplexing...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https...

  9. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...

  13. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...condition that can apply enough pressure to select for variants of interest, though. A selection can take the ...

  15. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...

  16. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  21. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  22. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...

  24. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ...

  26. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived...

  29. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

  31. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...

  33. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

  34. Pooled Library Amplifications

    Type
    Blog Post
    ...unique plasmids does your library contain?), the variance of size of plasmids in your library (Are the inserts... Library Type Name Number of Plasmids Variable Region Size DNA per Amplification Amplification...

  35. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...human disease. However, the rate of transfer was variable. They measured transfer rates by comparing the... of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to...

  38. Transferable Skills: Negotiation

    Type
    Blog Post
    ...covered separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement...  Maria Genco...

  41. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

  42. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...

  43. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain...

  45. CRISPR Plasmids - Parasites

    Type
    Collection
    ...disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African...

  47. Plasmid Collections

    Type
    Collection
    ...Collection Cell Migration Consortium Impact of Genomic Variation on Function (IGVF) Consortium Krüppel-like factors...

  48. Optogenetics Plasmid Collection

    Type
    Collection
    ...light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered or identified...

  49. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...

  50. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is... Good luck! This post was originally written by Maria Soriano-Carot in September 2016 and was updated ...
Showing: 151 - 200 of 389 results