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We narrowed to 400 results for: pho;

Showing: 151 - 200 of 400 results

  1. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...the opsins will receive sufficient quantities of photons to become activated. Light is able to penetrate...between patch cable and implant. A steady stream of photons from the laser to the patch cable to the ferrule...insensitive. If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity...

  2. MXS Chaining

    Type
    Blog Post
    ... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

  3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...further simplifies lab work if you like to bring your phone in the lab, though, honestly, this still grosses... out. Maybe it’s just me – I didn’t get a smart phone until after I had finished my PhD and left the lab...but is there a sterile, clean way to bring your phone into the lab? Maybe having a dedicated lab-use tablet...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ... FP scaffolds with either intact or darkened chromophores. These probes are both intrinsically fluorescent...The issue with these FPs is that they are less photostable than GFP. In addition, antibody-based amplification...contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with very specific reactivities...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver staining or SYPRO...purified and non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are...transfection. PCR products are analyzed by gel electrophoresis to ensure the correct banding pattern and sizes...

  7. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...that prefer a more economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach...812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination of cell cultures...Cytotechnology 39.2 (2002). 75-90. Pubmed PMID: 19003295. 4. Uphoff, C.C., Drexler, H.G. “Detection of mycoplasma contaminations...

  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq ...not always achievable. Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate...

  9. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...

  10. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation. Generally, I...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...protein characteristics such as the brightness, photostability, maturation time, and acid sensitivity of various...sensors for cAMP feature exceptional brightness, photostability and dynamic range: characterization of dedicated...PMC490022. Shaner, Nathan C., et al. "Improving the photostability of bright monomeric orange and red fluorescent...

  12. March for Science

    Type
    Blog Post
    ...Stephanie Hays, a scientist with a passion for photosynthetic communities, microbial interactions, and science...Institutes of Health endanger scientists’ ability to uphold their side of the bargain and do the research required...University. She is a scientist with a passion for photosynthetic communities, microbial interactions, and science...

  16. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...the RNAs from cellular defenses against ssRNA: phosphatase treatment, substituting cytidine with 5-methylcytidine...pluripotency, but they also take less time to complete. Morphology is a simple and fast to evaluate. iPSCs should...little cytoplasm. Positive staining for alkaline phosphatase, expression of embryonic proteins, and promoter...

  17. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ...festivities! Help us spread some cheer by snapping a photo of you and your labmates celebrating the holidays...Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab throughout the month of...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...party, we shared memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured...over the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...of application and aid in visualization of the morphology of individual neurons, including dendritic and...vivo, microinjection, pressure injection or iontophoresis (applying small electric currents via inserted... project but neurons were traced with AAV2. Two-Photon imaging was used to generate a 3D finemap of neurons...

  20. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...to isolate two proteins from the jellyfish’s photoorgans; the first, which he called aequorin, gives off...hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. and Katushka/mKate...

  21. Editor's Choice, September 2016

    Type
    Blog Post
    ...and provides a brief rundown of Sean’s top five smartphone apps you can use in the lab. Whether you’re tired...Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the...

  23. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

  24. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...a hassle. You finally make it through a 20-step phone tree, only to be left on hold for an hour. When ...the chance to stop what you’re doing and make a phone call or write out an email. You need help right ...

  28. An Addgene Summer Internship

    Type
    Blog Post
    ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

  33. RUBY-Red Siliques

    Type
    Blog Post
    ... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...

  36. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For more specific procedural...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

  37. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

  41. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

  46. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...and cells. They can be used either with or as fluorophores in a wide variety of colors.  To antibody or... the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs Antibodies 101: Introduction...

  50. Working with Nuclear Receptors

    Type
    Blog Post
    ...actions of the whole complex.  For example, the phosphorylation status of SRC-3 defines its association with...
Showing: 151 - 200 of 400 results