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We narrowed to 535 results for: try;

Showing: 151 - 200 of 535 results

  2. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant excitation/emission...bleeding, or spectral overlap, and why you should try to avoid or reduce it whenever possible. Finally,...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Flow Compensation...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...reporters, and/or dye stains used in your flow cytometry experiment are known as your "panel". In this ...from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation...degree. The phenomenon of spectral overlap in flow cytometry is commonly referred to as bleeding. When two ...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...have mastered this technique so it might be worth a try, especially since the potential rewards could be ...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...reassignment–Expanding the chemistry in biology." Chemical Science 6.1 (2015): 50-69. Royal Society of Chemistry. 3. Ermolenko...distance between the donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected by intervening...23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as a spectroscopic...spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID: 354506. 12. Vilensky...

  8. Plasmid Cloning by PCR

    Type
    Blog Post
    ...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...

  18. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...engage my students in real-world lab activities? I’m trying to inspire them and also keep up with all the new... the summer with dozens of teachers around the country. How you can get involved with BioBuilder Details...

  19. How Addgene Creates Content

    Type
    Blog Post
    ...absolutely everything was correct. Final touches We try to use figures and images to help illustrate our ... as well as consults with microscopy and flow cytometry experts for the panel recommendations. A few discussions...

  20. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress...researchers or students beginning to learn about biochemistry. The least this app will do is allow you to ...

  24. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...next section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational... to other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in ...

  25. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...the post. And the blog writing tips are elements I try to include in each post I write for the Addgene blog...started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media as a scientist...) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social media as...

  27. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...and ready to deliver. Ready to give biosensors a try? Peruse our available biosensors or build your own...biosensors span the gamut of applications within chemistry, biology, and physics, in this blog we will focus...

  28. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...others are dubious enough that they don’t give it a try. Optimization Why is GCE so complicated? Well, while... Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/...

  34. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...

  35. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...toolbox you are putting together?  Voigt: We’ve been trying to get everything to the scale of building synthetic...is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something has to be...program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon was built on...We did demonstrate that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems...

  37. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...could bind not only the endogenous proteins you are trying to block but your primary antibody as well.  Next...

  39. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

  46. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

  48. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

  50. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... also be determined empirically but it’s best to try those that have already been published first. Because...cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29):25848-58. doi: 10.1074/jbc.M111.253674...
Showing: 151 - 200 of 535 results