We narrowed to 539 results for: try;

... a microscope’s eyepiece can take some practice. Try to keep both eyes open and adjust the position of...

...lectures should be “brains-on”, but as much as I try being compelling, interactive, and application-driven...

...comparisons.  We very much appreciate feedback, so try out the site and contact us if you have any ideas...

... we are all so good with video calls, how about trying your hand at a video presentation? Most video platforms...

...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...

...any labs performing genome editing experiments to try nucleofection as (in our hands) this technique supports...

... on this topic and for additional best practices try "Why Aren't You Delegating" in the Harvard Business...

...if you want the brightest signal, you may need to try several proteins in your system of interest. Finally...

...diagnostic restriction digest. For your digest: Try choosing unique enzymes. Enzymes that only cut once...

...any special tips or tricks for someone looking to try these optogenetic tools out for the first time? Boyden...

...best astrocyte differentiation protocol! Ready to try it out for yourself? Check out the MORF Collection...

...engage my students in real-world lab activities? I’m trying to inspire them and also keep up with all the new... the summer with dozens of teachers around the country. How you can get involved with BioBuilder Details...

...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

...be in and usually self-limiting effects kick in trying to resolve the inflammatory state by inducing immunosuppressive...

...with open data, materials, and code Only when you try to replicate the work of other researchers (or your...

...absolutely everything was correct. Final touches We try to use figures and images to help illustrate our ... as well as consults with microscopy and flow cytometry experts for the panel recommendations. A few discussions...

...technique, I try to look for a resource to help me. I’m still waiting for a mass spectrometry app. I digress...researchers or students beginning to learn about biochemistry. The least this app will do is allow you to ...

...advance and make a note of those you would like to try and meet. Don’t worry about contacting someone much...

...software tools, what problems various tools are trying to solve, and finally how we might proceed in the...efficacy is still a concept that only a few tools are trying to solve. Advanced functions: These functions...different scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool...

...and ready to deliver. Ready to give biosensors a try? Peruse our available biosensors or build your own...biosensors span the gamut of applications within chemistry, biology, and physics, in this blog we will focus...

...others are dubious enough that they don’t give it a try. Optimization Why is GCE so complicated? Well, while... Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/...

...open-source modular optical toolbox openUC2 are trying to overcome this hurdle by taking these limitations...

... type tested. To conclude, we recommend that you try a couple of different fluorescent proteins when protein...

...with fluorescent proteins, the best advice is to try a couple of promising variants to check how they ...

...next section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational... to other antibody formats. This low barrier to entry makes polyclonal antibodies a common reagent in ...

...2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities....

...the post. And the blog writing tips are elements I try to include in each post I write for the Addgene blog...started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media as a scientist...) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social media as...

...expression levels, give these stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo...

...example where a selection would work is if you are trying to identify genetic conditions which convey resistance...

...Resources on the Addgene Blog Learn How Scientists Are Trying to Shape the Future of Research Learn How Notions...

...originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...

...toolbox you are putting together?  Voigt: We’ve been trying to get everything to the scale of building synthetic...is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something has to be...program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon was built on...We did demonstrate that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems...

...could bind not only the endogenous proteins you are trying to block but your primary antibody as well.  Next...

...autophagy of your protein of interest. Are you ready to try out Rosella? Both the cytosol-targeted and mitochondria-targeted...

... parents. If both of the mutations that you are trying to cross are located on homologous chromosomes ...

...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

...role at Addgene is “Words are important!”  If you try any of these approaches in your lab or workplace,...

...have many levels of complexity, especially when trying to explore the links between genetics and anatomy...

... next? First, check the submission deadline and try not to panic if it's due in a few days, weeks, or...

... a sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream...

... purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM MgCl 2 and/or 1µl DMSO to each...

...can grow in spite of a hostile Penicillium fungus trying to kill them off. While antibiotic resistance-mediated...

...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

...efficiency. Before constructing your own FRET probe, try searching PubMed for articles describing the FRET...

...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

...getting sub-optimal plasmid yields from your prep, try our tips below! How to optimize your plasmid prep...

... also be determined empirically but it’s best to try those that have already been published first. Because...cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29):25848-58. doi: 10.1074/jbc.M111.253674...

...individual members, many opinions may exist. I learnt to try and bring a group of people to an overall consensus...

...to get your point across. Some supervisors might try to intimidate you with negative comments (hostile-aggressives...

... 3,000 times each month. I looked this up after trying out the new protocols.io beta platform for sharing...pricing options for academic labs, departments, industry, and more to fit every lab's needs. Plus there...