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We narrowed to 319 results for: virus

Showing: 151 - 200 of 319 results

  1. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  3. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells....also like... AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...allow full precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer...cleared lysate to the tube containing the resuspended virus from step 12 above. Add 50 units of benzonase per...

  7. New Viral Vectors - March 2024

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Sontheimer EJ (2018) All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis...and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038...

  9. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ... use in screening experiments; large amounts of virus are required to ensure that you get full library...backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR v2 is key ...

  10. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Technology You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction The AAV... x 10 13 GC/mL and we use an internal reference virus that is 1 x 10 13 GC/mL. We recommend always using...detection and quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...prevent substances in the blood, like proteins and viruses, from crossing into the brain. While helpful from...BioRender.com. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...T.S., Miles T.F., Gradinaru V. Adeno-Associated Virus Toolkit to Target Diverse Brain Cells. Annual Review...T. F., & Gradinaru, V. (2022). Adeno-Associated Virus Toolkit to Target Diverse Brain Cells. Annual Review... C., Balaram, P., … Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling...Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency...

  14. Plant Plasmids and Resources

    Type
    Collection
    ... Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome editing across... improved vector system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome ...

  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol Lentiviral... or not you've efficiently generated infectious virus, plasmids expressing both the Cre and Flp recombinases... Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of ...pAdenoX from Clontech and can be used to generate adenovirus expressing a variety of reporters as well as ...

  16. General Transfection

    Type
    Protocol
    ...Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction...cell line is critical for obtaining high levels of virus. HEK293T cells should be split 3 times a week: Monday...

  17. Editor's Choice, September 2016

    Type
    Blog Post
    ...of you who are not microbiologists, phages are viruses that infect bacteria and that can do so with exquisite...

  18. Editor's Choice, August 2016

    Type
    Blog Post
    ...Biology Adapting Toehold Switches to Detect Zika Virus As always, if you have any questions or suggestions...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...! Delivering prime editors (PEs) via engineered virus-like particles (eVLPs) offers an option to transduce...Palczewski, K., & Liu, D. R. (2024). Engineered virus-like particles for transient delivery of prime editor...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...activation domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing TetO elements... of VP16, the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988...

  22. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...of national boundaries. To be able to beat this virus, we will need to do the same with science,” said...groups have focused on iterating on methods for coronavirus research, sharing resources for COVID-19 research...our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene...

  23. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  24. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...up their own CRISPR arrays. On the other hand, viruses can evolve to evade CRISPR by mutating these sequences...

  27. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... genome (genome engineering) Produce synthetic viruses that can be used in research or for therapeutics...to antibiotics, degradative functions, and/or virulence. All natural plasmids contain an origin of replication...

  28. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  31. Deisseroth INTRSECT Collection

    Type
    Collection
    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based...recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases 10–14 . Figure...principles for working with INTRSECT recombinases, viruses, and important controls to consider as part of ...

  33. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...does not raise the safety concerns associated with virus work. Fourth, working directly with proteins removes...

  34. Plasmid Collections

    Type
    Collection
    ...Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids ...

  39. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...replication. Cell lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T...

  40. AAV Molecular Tools

    Type
    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

  43. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...mechanism to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery...

  47. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...library to screen for genes involved in West Nile Virus (WNV) induced cell death. The Wu lab library consists.... PubMed PMID: 26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi... Addgene’s repository. The genes, from the Zika virus Asian genotype PRVABC59, were obtained from the ...using direct transfection precluding the need for virus. Furthermore, dCAS9-VPR expression is under the ...First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors...use the library, the authors packaged it into lentivirus and used the resulting lentiviral library to ...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing...

  48. Recombinases AAV Preps

    Type
    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...
Showing: 151 - 200 of 319 results