...when using highly competent cells. If you used 100-1000 ng of total DNA in a ligation you will often get...

... Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool...

... Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive...Dlx dTomato Cre dependent 1, 2, 5, 9, rg* Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH Syn EGFP Cre dependent...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Reagents 1X PBS Microcentrifuge...

...middle is hundreds, and the third is tens. Therefore, 1000 µL would read as 100 from top to bottom (as shown...Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger at the top, which is used...tip off of the pipette. This side view photo of a P1000 pipette shows the volume adjustment ring right below...come in standard sizes: P2, P10, P20, P200, and P1000. The table shows the pipette with the appropriate...This image shows the P2, P10, P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume... 2 to 20 µL P100 10 to 100 µL P200 20 to 200 µL P1000 100 to 1,000 µL Each pipette should be used with... dispense the correct amount of liquid reagent. P1000: The first red digit is thousands of µL, the middle...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...

...ID PI Description MORF library 137000, 192821, 1000000218 Zhang A collection of human transcription factor...

...in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain modular...

...Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation...

...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S....

...pipettes Ice bucket to hold antibiotic Antibiotic at 1000 x concentration disolved in the appropriate liquid...antibiotic to the molten gel mix, you should create a 1000x stock solution. For example: If you’ll be preparing...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...95 uL 20X 200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution ...

...in response to green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids ...

...Cre dependent 5, 9 Tian Calcium Sensor: Twitch2B 100040 pAAV.hSyn1.Twitch2B.WPRE.SV40 Syn Twitch2B none...

...MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 µL) Bacti Cell Spreaders (VWR, 60828... 100 x 100 ÷ 0.1 This number should be at least 1000X greater than number of perturbations in library....least 10 colonies on the most diluted plate for 1000x coverage of a library of 100,000 plasmids. Colonies...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette...

...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S....

..._CTD_del321-343 TARDBP His T7 ALS Nicolas Fawzi 100092 pET28-his-3C_Tau352 MAPT His T7 Parkinson's, FTD...

... or more from the same tube, but unlike using a p1000 tip it will give you more control of where you’re...