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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 151 results for: 199

Showing: 61 - 80 of 151 results

  1. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...replication in different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control... Doran, K. S., Konieczny, I., & Helinski, D. R. (1998). Replication Origin of the Broad Host Range Plasmid...

  2. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...can have endogenous GUS-like activity (Hu et al., 1990). We commonly use pSB161 - pL2_pSB90_2x35S::GUS:...activities in seed plants." Plant cell reports 9.1 (1990): 1-5. PubMed PMID: 24226366. Lee, Min Woo, and ...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing viral capsids...production of recombinant AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J....

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...vectors for gene editing in mammalian cells since 1998. In that year, Russell and Hirata from the University...targeting by viral vectors." Nature genetics 18.4 (1998): 325. PubMed PMID: 9537413. PubMed Central PMCID...

  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ....0036  Proba K, Wörn A, Honegger A, Plückthun A (1998) Antibody scFv fragments without disulfide bonds...Biology 275:245–253 . https://doi.org/10.1006/jmbi.1997.1457  Sandomenico A, Sivaccumar JP, Ruvo M (2020) Evolution...

  7. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...mechanism. RNAi was a landmark discovery in the 1990s and quickly rose to popularity. Compared to CRISPR...., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic interference by double-stranded...

  8. AAV ddPCR Titration

    Type
    Protocol
    ..., 10153-688 Mini Centrifuge, Thermo Scientific, 10199-452 Ice bucket 96-well freezer blocks (x 3) Reagents...

  11. Lentivirus ddPCR Titration

    Type
    Protocol
    ..., 10153-688 Mini Centrifuge, Thermo Scientific, 10199-452 Reagents GeneJet Genomic DNA Purification Kit...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This work also introduced the TRE, placing seven...-repressible system. Tetracycline On (Tet-On) In 1995, Gossen et al. used random mutagenesis to identify...developed by Clontech, based on Resnitzky et al., 1994). TRE3G promoter : also optimized into P TRE3GV ...opens in a new window) Gossen, M., & Bujard, H. (1992). Tight control of gene expression in mammalian ...Bender, G., Müller, G., Hillen, W., & Bujard, H. (1995). Transcriptional activation by tetracyclines in...Resnitzky, D., Gossen, M., Bujard, H., & Reed, S. I. (1994). Acceleration of the G1/S phase transition by expression...1669–1679. https://doi.org/10.1128/mcb.14.3.1669-1679.1994 (Link opens in a new window) PMID: 8114703 (Link...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...conversion tract length in Drosophila. Elliot et al. (1998) suggests mammalian cells' tract lengths seem much..., Winderbaum, J., Nickoloff, J. A., & Jasin, M. (1998). Gene Conversion Tracts from Double-Strand Break...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...tracing, biotinylated dextran amines (Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate...2536188. Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde tracer ...

  15. Brain Armamentarium

    Type
    Collection
    ...interneuron-targeting enhancer Gordon Fishell Viviana Gradinaru 199777-PHPeB AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T...pathway D2-MSNs Jonathan Ting Viviana Gradinaru 199776-PHPeB AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(...pathway D1-MSNs Jonathan Ting Viviana Gradinaru 199775-PHPeB AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T...medium spiny neurons Jonathan Ting Viviana Gradinaru 199774-PHPeB AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2...medium spiny neurons Jonathan Ting Viviana Gradinaru 199773-PHPeB AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3...

  16. Caltech Systemic Capsids

    Type
    Collection
    ...(Alias: CN2610) minBG SYFP2 Control AIBS , Ting 199773 AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA...(Alias: CN3863) minBG SYFP2 Control AIBS , Ting 199774 AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3...4X2C-WPRE3-BGHpA minBG iCre expression iCre Ting 199775 AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA...) minBG iCre(R297T) expression iCre AIBS , Ting 199776 AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)...) minBG iCre(R297T) expression iCre AIBS , Ting 199777 AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA...

  17. Rett Syndrome

    Type
    Collection
    ... of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered by Adrian Bird's lab. (Link...Link opens in a new window) PMID: 1606614 1999 - Huda Zoghbi's lab identifies MECP2 as the genetic cause...Rett syndrome. (Link opens in a new window) PMID: 29019980 Return to top Animal Models Studies of the MECP2... window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by mutations in X-linked...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...SYFP2 DRD1 Medium spiny neurons (D1-MSN) Striatum 199776 pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA AiP13779...SYFP2 DRD2 Medium spiny neurons (D2-MSN) Striatum 199777 pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA AiP13781 ...AiE0784m SYFP2 Medium spiny neurons (MSN) Striatum 199774 pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA AiP13905...AiE1419m SYFP2 Pvalb_Pthlh interneurons Striatum 224199 pAAV-AiE1419m-minBG-iCre(R297T)-BGHpA AiP15508 ...

  19. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Bac-to-Bac Baculovirus Expresion vector 26117 pNIC-CH EF199843 Hexahistidine tag, AmpR 26103 pNIC28-Bsa4 EF198106...Hexahistidine tag with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage,... Z-basic, TEV cleavage, AmpR 26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... 26884157 Aronshtam, et al. Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast...Nishimura K, et al. Nat. Methods 2009. PubMed PMID: 19915560 TRIBE: Targets of RNA-binding proteins identified...
Showing: 61 - 80 of 151 results