...couple of my favorites. Plasmids from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used...(basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively, if you prefer Golden... let them recover for a week, and then dip them again.  This won’t hurt them, and it will increase the...25902487. PubMed Central PMCID: PMC4426443. 4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors...

...pieces of DNA whose sole purpose is to ensure the propagation of themselves and their associated genes. These...first discovered in 1983 when Teru Ogura and Sota Hiraga found that if a mini-F plasmid carried a specific...specific 700 bp segment of DNA, the plasmid was propagated better than plasmids without this DNA. They eventually...biological inquiries. References Ogura, Teru, and Sota Hiraga. "Mini-F plasmid genes that couple host cell division...

...in absence of protein synthesis Ptrc-ccdA   Propagation of ccdB-containing plasmids Hte   High transformation... ccdB Survival 2 T1R   TOP10 derivative. For propagating plasmids expressing the ccdB gene (important ... DB3.1 Streptomycin  HB101 derivative. For propagating plasmids expressing the ccdB gene (important ... Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries...

...the information necessary for regulating and propagating life, there was no turning back. While the world... In this movie Ethan Hawke’s character revolts against class divide that is created by genetics.    Zinc.... The movie sees the USS Enterprise crew fight against a genetically engineered superhuman Khan. The novel...s0764-4469(00)01250-6 Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering...

...image. You could probably also do Western blotting against GFP expression, but I didn’t. 2. In the original...data not shown). Pro-Tips: 1.Designing primers against genomic DNA to amplify the gRNA targets was challenging...figure with the data). Using acrylamide instead of agarose gives much sharper bands. I compared two MOIs of...analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands. Conclusion Overall, making...

...was a great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear...cause breast cancer and infertility or to argue against unnecessary clinic regulations, evidence-based ...research is vital in combating misinformation and propaganda that limits access to full reproductive health.... I worried that, because we were speaking out against legislators and because the march had been so well-publicized...

...room with a confocal microscope, yet I was once again fascinated by the world that reveals itself when...finale of visual impressions was delivered by the ASM Agar Art Winners! It is absolutely fascinating to see...microbiologists. If you got inspired to produce your own agar art have a look at our Microbiology and Fluorescent...

...years, including an article in the BBC Wildlife Magazine. It is easy to see how these incredible images...featured in the National Geographic and Smithsonian Magazine, where the editor stated “Albert G. Richards, ...stunning images that is has been featured in Time magazine . Other smaller competitions are now taking place...

...notice the name Vladislav Verkhusha popping up again and again, and for good reason. We all know scientists...sections of the relevant papers, and then read them again. Most of the time, the answers will be there.   ...

...Cas as a powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive ...clustered repeat sequences operated as a safeguard against bacteriophages did not come to light until 2007...Cas systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution...

... strain Most E. coli strains can be used to propagate plasmids, but endA- E. coli are best for high ...make sure your growth conditions aren't working against you!  The culture inoculum Freshly streaked...do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly transformed cells...

...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on...

...and find an ideal antibody against Protein A and an ideal antibody against Protein B. The antibodies are...target and can be used with secondary antibodies against the new Fc region. In addition to increasing multiplexing...

... Pamela J. Hines PhD, Senior Editor at Science Magazine. Although we only walk one path at a time, the...spotted an opening for being an editor at Science magazine. That one I just could not resist applying for... Pamela J. Hines is a Senior Editor at Science magazine, published by the American Association for the...

...said, “Science is failing over and over and over again until you don’t.” As every researcher can attest...- within that process of failing, over and over again until you don't, of trying to understand your completely...understanding why your experiment has not worked…again…for the seventh time….   I find that once I sit ...

...you can compare the fluorescence of your sample against this curve to quantify your DNA prep. Though this...will calculate your sample concentration for you. Agarose gel electrophoresis Though not the quickest way...way to quantify DNA, you can use the agarose gel method to not only find out how much DNA you have, but...

...Paris and New York. We want YOU to join the fight against coronavirus We are just at the beginning of understanding...next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in various... UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our FedEx guy...

...conformational changes in proteins? How can information propagate through a structured material over a long distance...amplified tens or hundreds of thousands of times by propagating through an enormous lattice of identical subunits...

...specific sites and 2) running the reaction on an agarose gel to determine the relative sizes of the resulting...unique enzymes (HindIII and BamHI) and run on an agarose gel. The resulting gel image includes a 1kb ladder...When uncut plasmid DNA is isolated and run on an agarose gel, you are likely to see 3 bands. This is due...

... to generate two more OptoNBs, one against mCherry and one against GFP. Insertion of AsLOV2 into loop ...improve this process. The team used nickel-coated agarose beads coated with his-tagged OptoNBs. OptoNB coated...