We narrowed to 1,088 results for: ANT

... new window) More Resources at Addgene: Recombinant Antibodies for Epitope Tags and Other Markers AAV ... of stochastic combinations of cell-filling antigenically distinct protein tags (or "bits"), creating ...over 100,000 unique combinations detectable by antibodies. All of the PRISM protein tags have been validated...corresponding primary and dye-conjugated secondary antibody (as listed in Park & Sheridan et al., 2025). These...

...Yeast Expression mGreenLantern 503 514 73 5.6 14 min Monomer (A206K) pcDNA3.1-mGreenLantern - Mammalian Expression... Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern - Yeast Expression Clover... is the product of exctinction coefficient and quantum yield divided by 1,000. Note that the effective...10His-mStayGold (E138D) - Bacterial Expression pJRA110 - Plant Expression mStayGold 499 510 136 4.8 Monomer pRSETB... to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to...

... to embryonic stem cell use and has the major advantage that iPSCs can potentially be derived from patient-specific...mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology. Browse Addgene's collection...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...Mouse Direct reprogramming of fibroblasts into antigen-presenting dendritic cells. Sci Immunol. 2018 Dec...Lentiviral Mouse Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell...

...hormones or metal ions, scientists have adapted an antibiotic resistance operon from E. coli : the tetracycline...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...mutating the amino acid residues of TetR that were important for tetracycline-dependent repression led to a...). rtTA3 and Tet-On 3G : further improved rtTA variants with lower background activity and greater sensitivity...#85972 for hygromycin selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid...Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber...

...-type. However, variants such as the P.1 (Gamma variant) and the B.1.351 (Beta variant) were much less...B.1.351 variant neutralization escape The lab saw that the B.1.351 variant was very resistant to vaccine...differed depending on the variants (Garcia-Beltran et al., 2021). Antibodies from individuals who received...vaccine could neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429 (Epsilon...on the variant. The lowest levels of cross-neutralization occurs in the P.1 and B.1.351 variants tested...circulating variants. This allowed us to create pseudoviruses that represent lineages and variants that are... distinct from SARS-CoV-2 and its variants. Yet, the B.1.351 variant, which has only 9 different mutations...

...Many Benefits of Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal...Fvasconcellos.     How are Recombinant Antibodies Made? To make recombinant antibodies, you first need to know...definition of recombinant antibodies are essential for good reproducibility. Non-recombinant antibodies are notorious...I predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...the scientific toolbox. What are Recombinant Antibodies? Antibodies are used by scientists around the...Bottom) Recombinant monoclonal antibodies are encoded in plasmids and produce a single antibody recognizing...recognizing one epitope.   Recombinant antibodies are monoclonal antibodies that are generated in vitro ...

...other mutants Chronos, Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR...eNpHR and variants InSynC (non-opsin) Jaws WiChR stGtACR iC++ OPN3 PdCO PPO Bidirectional BiPOLES Promoter...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection Disease...Browse depositor collections of antibody, CRISPR, disease research, visualization, protein expression... Simpson Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project ...

...designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed... for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans...tool for genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR ...

...protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical...proteins with disulfide bridges such as antibodies. MCP-tag is a mutant of ACP-tag, and is labeled by SFP Synthase...

... genome into a host cell. Scientists can take advantage of this to stably integrate transgenes of interest...Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest?...pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells. See the Weinberg...Weinberg Lab page for additional resistance variants. Bob Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...

...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...uncontrolled cell growth and proliferation, including an abundant supply of macromolecules and energy, as well as... as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears to signal... mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93...

...though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical Titer Small...serotypes: AAV1 AAV2 AAV5 AAV6 AAV8 AAV9 AAV11 AAVrg Want more information before choosing a serotype? Learn... on Request preps is all-inclusive of several important steps: MTA facilitation for the transfer plasmid...you need more assistance with troubleshooting, we want to hear from you! Email us at [email protected] ....

...single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast ... a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9... express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

...during Development and Regeneration - Periklis Pantazis Lab Multibow: digital spectral barcodes for cell... loss and ablation of previously resistant cell types. CreLite - George Eisenhoffer Lab. ...for Zebrafish Receptors and Zebrafish Monoclonal Antibody plasmids - Gavin Wright Lab. Collections of plasmids...that encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted...

...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...properties of the final vector preparation, the quantity of gram-negative bacterial endotoxin is ensured...within representative vector preparations was quantified with electron microscopy after negative staining...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

...available as cgDNA, we hope to aid scientists who want to immediately start cloning upon arrival of their...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...

...gene targets. To accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

...See below for plasmid kits and other resources relevant to these organisms, or use our Search page to ...Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...