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We narrowed to 1,087 results for: ANT

Showing: 61 - 80 of 1087 results

  1. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection...Addgene blog Antibodies 101: Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...single-fluorophore-conjugated antibodies. These methods can include labeling markers with multiple proteins/antibodies, targeting... to antibodies and 'staining' to refer to small molecule dyes. Indirect labeling While antibodies used...fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated antibodies may exist...fluorophore-conjugated secondary antibody is added that binds the conserved, host species-specific (“anti-rabbit”) region...region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

  2. Antibodies 101: Flow Cytometry Controls

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    Blog Post
    ... from your antibody panel. They are made up of compensation beads and a conjugated antibody. Compensation...your sample. The isotype antibody will be virtually identical to the antibody for your marker of interest...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs. Spectral...Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection        ...Depending on the markers you are looking at and the antibodies you are using, you may need to include additional... synthetic beads that can bind to a conjugated antibody and beads that are unable to bind to them: like...The compensation beads can bind to any primary antibody you add. Single color controls are used to help...

  3. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ... IPI anti-integrin toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either...Addgene's Antibody Guide Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...Table 1: IPI antibodies  Antibody Target Proteins Reactivity Isotype Source Species Anti-Integrin...unique antibodies that bind discrete subunits or particular subunit pairs. Some of the antibodies in the...property in an antibody is extremely rare. While many function-blocking integrin antibodies exist, almost...are chimeric antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All...monoclonals, we anticipate that our chemically identical hybridoma-derived antibodies will also work in...

  4. Antibodies 101: Reading a Flow Plot

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    Blog Post
    ...license.    In the third plot, anti-CD14 and anti-HLA-DR antibodies conjugated to fluorescent markers...Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies  ... is a cell, but it could be debris or unbound antibodies.) Each event read by the machine is plotted as...conceptualize where the populations are, try drawing a quadrant over the plot that divides it into four, using...different populations by imagining (or drawing) a quadrant over the dot plot. Created with biorender.com....Biorender! While a helpful visualization tool, a quadrant will often be too clean for real-world data. Most...using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/or cellular...

  5. Important Considerations When Using AAVs

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    Blog Post
    ...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should ...value greatly. Applying this mantra to AAV selection, there are three important factors related to location...and using AAVs have in common? The three most important items when looking for a house and choosing an...location, location! Just like this common real estate mantra, the choice of serotype (which determines which...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...retrograde transport) or towards the synapse (anterograde transport), which is a possible mechanism for...and 9, not only do they undergo retrograde and anterograde transport, but they also have similar transport...

  6. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post
    ...differences include the ferrule implants and patch cables. One huge advantage of using rats is their superiority...but, in today’s post, we’ll discuss some of the important considerations you should think about when developing... preference.   Historically, ICSS was done by implanting an electrode and non-specifically electrically...literature to find all the applications of optogenetics relevant to your field. Ultimately, your question should...stimulation could induce seizures or some other unwanted phenomenon or it could reveal something fascinating...could move your project onto the exciting—and unanticipated—road of discovery. Monitoring behavioral output...interested in studying a brain region that may be important in memory, an appropriate behavioral test might...

  7. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    .... Other relevant blog posts 3 Challenges in Plant Synthetic Biology Quick, Versatile Plant Transgenesis...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding...shaped the plants that we grow and eat, and genetic engineering will continue to improve plant nutrition...inversely proportional to size, and many plant scientists want to transfer large genes, smaller binary ... is functional in a variety of plant species. The kanamycin antibiotic resistance gene is controlled by...PMCID: 4045853.  Find other plant resources at Addgene Check out Addgene’s plant vectors! Find the plasmids...nutrition, yield, and pest resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens...

  8. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post
    ...Viva la (bacterial) resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill...common antibiotics used in labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be...the antibiotics listed in the table below. Note, in this post we'll focus primarily on antibiotics against...how to prepare antibiotic stocks, see Addgene's Reference Page. How else can antibiotics be used in the...scientist to select bacteria that are only resistant to the antibiotic that remains intact. Tips and tricks ...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...

  9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...this logic, ω-dCas9 variants recognizing multiple PAMs should have a fitness advantage over proteins with...flat out better than SpCas9. I want everyone to test it because I want to know the answer", says Liu. ...work, there are other options. Commonly used Cpf1 variants, with a  5’ TTTV PAM, improve targeting in AT-...respectively. Researchers also engineered SpCas9 variants with alternative PAMs like NGAG and NGCG and SaCas9...phage-assisted evolution to generate and select for Cas9 mutants with PAM flexibility. In this system, the mutagenesis...promoting survival of only the broadest-targeting variants. For the final round of evolution, they used a... a no-G PAM library to enrich for Cas9 variants with activity on non-NGG PAMs, generating xCas9 3.0-3.13...

  10. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ...expression of antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana...transgenic plant can take even longer, a transient plant leaf transformation process could save the plant biologist...Browse our Plant Plasmids and Resources page Find synthetic biology plant plasmids Find CRISPR plant plasmids...vincristine, are regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The ultimate goal of...to enhance their production either in the plant or in plant tissue cultures.   C. roseus is not a model...pathway. Why plant scientists do transient leaf transformations The development of transgenic plants is a common...the plant sciences “transient transformation” usual means that neither a stable cell line or plant is ...

  11. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post
    ...ahead of time that you want to probe your blot with multiple antibodies. You’ll want to set up your blot ...starting with the least-abundant protein and working toward the most abundant. If the abundance of your...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...without a re-wetting step. Important: do not dry your membrane with antibodies on it if you plan to reprobe... avoid retaining residual antibodies. If you have already applied antibodies and need to dry the membrane...stripping solution uses low pH to disrupt antibody binding to antigens. Ingredient Concentration or ...you don’t see any signal, you’ll want to check for the primary antibody by re-blocking your membrane and...

  12. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ...Addgene blog Antibodies 101: Flow Cytometry  Antibodies 101: Reading a Flow Plot Antibodies 101: Conventional...case through conjugated antibodies targeting the cell surface markers CD11 (anti-mouse CD11b-BV510), which...looser gate here. I am not worried about including irrelevant events in my analysis, as these will get excluded...Gating Having gated my single lymphocyte cells, I now want to exclude all dead cells from my analysis. For ... protect themselves from it. So here, I actually want to gate on my negative, unstained population, which...experimentally-specific gates. Two-dimensional staining (quadrant gating) For our last example plot, I will show...present on dendritic cells but not T cells, and CD8 (anti-mouse CD8b-PerCP-Cy5.5), which is present on cytotoxic...

  13. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ...Reactions Anti-drug antibodies (ADAs) are formed when the body recognizes a drug as a foreign antigen, triggering...replicate the complexity of antigen processing events that take place within an antigen-presenting cell like ...Beijnen, J. H., & Schellens, J. H. (2016). Antidrug Antibody Formation in Oncology: Clinical Relevance .../s12929-019-0592-z  Resources Antibodies 101: Introduction to Antibodies CRISPR in the Clinic   ...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...cancer to autoimmune disorders. Antibodies administered as drugs are still immunogenic, meaning that they elicit...of immunogenicity can help evaluate risk of an antibody.  In silico prediction of adverse reactions In...

  14. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The... as SHuffle cells, are already resistant to this antibiotic due to stable integration of resistance genes...-in-one antibiotic — it is usable in eukaryotic cell culture, yeast, bacteria, and even plants! The zeocin...host DNA damage. Choosing an antibiotic resistance Choosing the best antibiotic resistance ultimately comes...pay attention to antibiotic selection. If you feel like multiple of the above antibiotics would work for... the pros and cons of each antibiotic and happy selecting!   Antibiotic Resistance Stability Time ...Over-Agar Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post
    ... blog Antibodies 101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies 101: Buffers...Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...a given experiment, it is important to consider how many parameters you want to use in a given experiment...detector using a series of mirrors and filters. Importantly, conventional flow cytometry uses one detector...conventional and spectral flow cytometry, it is important to consider the expression levels of the markers...two highly overlapping cellular markers, it is important to pair them with two fluorophores that have distinct...is critical that your controls and your sample antibodies are from the same batch, to avoid unmixing issues...

  16. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...bacteria, it is important to note that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs... is not naturally resistant but has become resistant somehow overtime. One fantastic example of intrinsic... spread of antibiotic resistant bacteria, check out this resource on preventing antibiotic resistance ...Addgene's Microbiology Pages What is antibiotic resistance? Antibiotic resistance is defined as microbial... to antibiotics designed to kill them or to impede their growth. When we hear the word ‘antibiotic’, very... drugs and anti-fungal drugs, because ‘antibiotic’ merely means that the drug prevents the growth of a...treat. Learn How Antibiotic Resistance Genes Are Used in the Lab How does antibiotic resistance develop...

  17. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post
    ...improve your grant writing skills. Good luck! Further advice on grant writing Since successful grant writing...with your work, your advisor may want to mentor you during the grant application process. When my PhD ...have no idea how to write a grant!”, “I don’t have time for this if he wants data by next week!”, and “...RFA contains all of the important information required for a successful grant application. It typically... advice on writing a grant proposal. "Murder Most Foul: How Not to Kill a Grant Application" by Vid Mohan-Ram...How to write a research project grant application" by the NIH Still want more information on the application...their advisor alone applies for these competitive grant applications; however, in some cases, your advisor...

  18. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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    Blog Post
    ...GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning is... most frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University...based on the daily needs of an advanced molecular plant science lab.” Introducing GreenGate As Lohmann ...required to generate multi-construct transgenic plants. In the most practical terms, what GreenGate can...Arabidopsis, it should in principle work in any plant species. Tricks of the trade Lohmann does have one...

  19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ... What are toxin-antitoxin systems? These toxic substances are part of toxin-antitoxin systems that are... while the antitoxin is unstable, meaning that the cells must continually produce antitoxin to avoid cell...will still inherit some toxin and antitoxin. However, the antitoxin will be degraded leaving the cell ...applications. The discovery of toxin-antitoxin systems Toxin-antitoxin systems were first discovered in 1983.... The encoded toxin-antitoxin system consists of CcdB (toxin) and CcdA (antitoxin) where CcdB inferes ...higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin systems and their...-based toxin-antitoxin systems. One of the most well known protein-based toxin-antitoxin system is MazE...

  20. Developing a cold tolerant E. coli using specialized chaperones

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    Blog Post
    ...these enzymes implies that even a minute quantity can significantly reduce the operational time for a given...efficiency.  Finding recombinant psychrophilic enzymes for the host The expression of recombinant proteins for... contained cold tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis... psychrophilic proteins. The primary problem we wanted to overcome was inefficient protein folding at ...well under suboptimal conditions. This means recombinant proteins can aggregate or precipitate out as ...would allow the stable folding and expression of recombinant psychrophilic proteins. As the figure below shows.... And thus was born ELSA - our happy lil’ cold-tolerant E. coli! Additionally, we’re also working towards...
Showing: 61 - 80 of 1087 results