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We narrowed to 94 results for: Blue fluorescent Protein

Showing: 61 - 80 of 94 results

  1. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...tool that controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine oxidation to...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...make. For example, large insertions, like a fluorescent protein knock-in, suffer from low efficiency when... your desired insertion feature (e.g., a fluorescent protein), you can amplify the desired region using... of the commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre,...purify Cas9 protein. The table below contains some of our favorites, all of which have blue flames. ... Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein and the separated guide RNA...options for templates - see the example at right using blue flame plasmid pmScarlet_C1! After creating the T7...alleles using long ssDNA donors and CRISPR ribonucleoproteins.” Genome Biol. 18 (2017) (1):92. DOI: 10.1186...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...when illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential...locations. C) Enrichment of dual-labeled proteins identifies proteins that had been trafficked from the “source...generated a fusion protein called iPAK4 that assembles into a slow-growing protein fiber in the cytoplasm... Figure 4: Time-resolved protein ticker tape. A) Design of iPAK4 protein ticker tape recording. B) HEK...labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody new tricks...recently used phage-assisted continuous evolution and protein engineering to generate seven new prime editors...., et al. (2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a. CLAP... a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling procedure...optimized strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered...study the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of...FRET to study the intramolecular dynamics of the proteins in solution and when associated with microtubules...generating other SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids ...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that...three possible fluorescent proteins, chosen for minimal spectral overlap, minimal protein aggregation, ...groups to monitor protein and cell ageing, as the spectrum shift occurs during Timer protein life due to a... synthesized (green) protein and organelles with older (yellow to orange) protein. Image courtesy of Roberta...1852-61. A quicker method for protein inactivation Your protein of interest does x, but it also participates...your endogenous protein of interest and co-expressing its replacement recombinant protein with a FKBP domain...outer membrane protein with a FRB domain (MitoTRAP). Rapamycin binds your recombinant protein (via FKBP) ...

  6. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...can visualize the protein via fluorescence or chemiluminescence, precipitate the protein out of solution... a single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived... the presence of proteins from samples containing a mixture of proteins. The proteins are separated based...to study protein-protein interactions. Flow cytometry Flow cytometry is used to detect proteins on the ...scientists to target specific proteins they’re interested in. Once a protein is targeted with an antibody...entirety of the target protein: instead, it recognizes a small portion of the protein, known as an epitope... the target protein because the antibodies will recognize multiple epitopes on the protein. However, each...

  7. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...determine how Protein A and Protein B interact. You read extensive research on the two proteins and come up... an ideal antibody against Protein A and an ideal antibody against Protein B. The antibodies are both ...to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B and successfully... anti-Protein A antibody and an anti-mouse IgG2a secondary antibody to bind to the anti-Protein B antibody...process the variable regions of a parental antibody (blue, rat) are cloned into a backbone expressing the ...are rat IgG1, then you could convert your anti-Protein B antibody to a mouse IgG2a Fc. The resulting chimeric...successfully visualize both proteins.     Figure 2: Two primary antibodies with the same isotype...

  8. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / protein database search and analysis...brain IHC.  FPbase: A data repository for fluorescent proteins and their properties.  Expasy: Bioinformatics...with Addgene. NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database ExPASy translation...six open reading frames UniProt: A database of protein sequence and functional information, such as expression...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common...-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector...Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further ...Another common scenario is fusing your protein to another protein, such as GFP, which allows you to...localization of your protein. Additionally, tags are commonly added to aid in protein purification. Just...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-...multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael Davidson...

  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...of a protein, a yes-no plot is the best-case result. For example, you have introduced a GFP protein into... of a protein while the green (top) has an upregulated expression (shift to the right). The blue (third...adjusted voltage, cells that did not receive the GFP protein are most likely to be clustered at signals below...not! When you are looking to study the level of protein expression, instead of its presence or absence,...These smears tend to occur when you are looking at proteins that are expressed regularly in cells and might...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...The orange peis the baseline expression of the protein of interest. Created with biorender.com. The...

  11. Antibody Guide

    Type
    Collection
    ...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

  12. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...researchers, from selective protein ligation through introducing ncAAs with protein modifications of interest...If you study proteins, you’re probably quite interested in the canonical 20 amino acids. But in your ...your quest to learn more about your protein of interest, you may find the available amino acids a bit…limiting... called unnatural amino acids) are placed in a protein site-specifically to alter its function, mimic ...biologically relevant modification, or add in fluorescent or chemically reactive side chains, all of which...relationships or, in more practical applications, allow a protein to be more effectively used in a technology or ...put all these together, the cell will produce a protein with a site specifically incorporated ncAA, ready...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein Activity...

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...mini counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...for Glypicans DNA editing with STITCHR Dynamic protein labeling with TurboCas Enhancer-AAV viral preps...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by ...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3...our bacterial plasmid set for expressing tagged proteins, and stay tuned for mammalian expression and AAV...therapeutic potential. Our partners at the Institute for Protein Innovation (IPI) recently released a set of highly-specific...antibodies against three of the six human glypican proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    ...for the amount of protein you are expecting.  Fluorescent proteins Fluorescent proteins, while more expensive...binding to your protein of interest. Protein preparation Before you can blot your proteins, you’ll need ...normalize your protein of interest to overall protein expression for each sample and protein. While ubiquitously...normalize your protein of interest to total protein expression. Total protein expression can be measured using...rely on primary antibodies conjugated to fluorescent proteins, instead of the more common chemiluminescent...consider the subcellular location of your protein. Proteins located in membrane-bound subcompartments,...considered if your protein is difficult to solubilize, such as a membrane-bound protein. There are many ...

  16. TALEN Plasmids and Kits

    Type
    Collection
    ... the protein. 49043 EMM67 49044 EMM71T Golden Gate TALEN 2.0 49401 pBlue-TAL Michal Zurovec pBlue-TAL ...vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...deletion from the N terminus of the wild-type TALE protein (previously shown to preserve the DNA-binding ability...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning... Golden Gate TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and...

  17. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors...inverted repeats (green) flanking the gene of interest (blue) in a plasmid backbone. A separate plasmid contains...

  18. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins are ...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification of ...vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice. Current...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...table above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific cation channel, activation...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...amino acids) fragments for protein-protein interaction assays. Each target protein is fused to either LgBiT...Constructs Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...Luciferase Complementation assays can report on protein-protein interactions, drug-induced changes, and more... about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns...Both firefly and Renilla luciferase have short protein half-lives, making them useful as transcriptional...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...ATP-independent, and a secreted version of the protein is available, making it ideal for a variety of ...

  20. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... are trying to remove from your sample is protein. Proteins absorb light to varying degrees across the...specific to proteins and why researchers traditionally use the A260/280 ratio as an indicator of protein contamination...and likely contaminants found in DNA samples. The blue lines in B-D represent the spectra for the specified...downstream experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own...how different contaminants impact these ratios.  Protein contaminants Whether you are purifying plasmid ... The A260/230 ratio can be a good indicator of protein contamination, but a number of other potential ...A260/280 ratio does not drop significantly until protein levels exceed 70% of the sample content (Loughrey...
Showing: 61 - 80 of 94 results