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We narrowed to 186 results for: Coli

Showing: 61 - 80 of 186 results

  1. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...gram negative bacteria include salmonella and E. coli. Because of their thicker peptidoglycan layer, gram... Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

  2. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...vaccinia virus DNA topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... that’s easier to express in the cytoplasm of E. coli, although this is not possible for all scFvs.   ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences...Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody Recombinant...

  5. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth...mutations per nucleotide per generation of wild-type E. coli, while only increasing the standard mutation rate...

  8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...nonmethylated PCR products are then transformed into E. coli to undergo nick repair. This method can work well...relying on in vitro assembly, they could make the E. coli do the work by using the principles of recombineering...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae...

  10. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...opposite, a uracil DNA N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI,... of 30 target sites. But the same strategy in E. coli produced different results. GBEs seemed to follow... by excising the freshly edited U bases — but E. coli instead filled those abasic sites with A's, producing.... In addition, they showed that UNG from both E. coli and C. elegans could be effective components of ...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... With MAGIC, scientists introduce an Escherichia coli donor strain into the microbiome to deliver a genetic.... The lab used DCDC to measure growth rate of E. coli for over 10 generations and showed that this method...species such as Snodgrassella alvi and Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira...

  14. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...plasmid multimerization in the naturally occurring E. coli plasmid ColE1 (Goebel & Helinski, 1968; Bazaral ...Bedbrook & Ausubel, 1976; Fishel et al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, ...harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi: 10.1016...Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). ...codon optimizing a human gene for expression in E. coli. Genes are also optimized for expression in mammalian...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...from the bacterial genome, and inserted it into E. coli to express and then test for any proteins that could...addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth...
Showing: 61 - 80 of 186 results