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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7 results for: Coli

Showing: 1 - 7 of 7 results

  1. Molecular Biology Reference

    Type
    Guide
    ... of E. coli strains below and recommend checking out Addgene’s blog posts about common E. coli lab strains...Elements Types of Plasmids Molecular Cloning Common E. coli Strains Common Antibiotics DNA Sequencing Sanger...For instance, most plasmids are replicated in E. coli and are relatively small (∼3,000–6,000 base pairs.... When a plasmid exists extrachromosomally in E. coli , it is replicated independently and segregated ...Common Bacteria Strains for Propagating Plasmids E. coli are gram-negative, rod-shaped bacteria naturally...many different naturally occurring strains of E. coli , some of which are deadly to humans. Most of all...all common, commercial lab strains of E. coli used today are descended from two individual isolates, the...

  2. Modular Cloning Guide

    Type
    Guide
    ...constructs constrained only by the ability of E. coli to maintain the final plasmid after transformation...purification tags and fluorescence proteins for use in E. coli for a variety of applications. EcoFlex MoClo Extension...backbones, and 6 destination vectors for use in E. coli for a variety of applications. CIDAR MoClo Parts...combinatorial design and expression tuning in E. coli . CIDAR MoClo Extension, Volume I Bacterial Expression...ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial Expression Christopher...and FBF (PUF) homology domain, for mammalian or E. coli expression. T2A Split/Link TurboID Kit Mammalian...

  3. Sequencing Primers

    Type
    Guide
    ...with E. coli araBAD promoter Forward pBAD Reverse GATTTAATCTGTATCAGG For vectors with E. coli araBAD promoter...

  4. Adenovirus Guide

    Type
    Guide
    ...AdEasier-1 cells (Addgene #16399) , which are BJ5183 E. coli cells containing pAdEasy. pAdEasy is a ~33 kb adenoviral...exposure to an antigen. AdEasier-1 cells BJ5183 E. coli cells containing the pAdEasy-1 packaging plasmid...

  5. Promoters

    Type
    Guide
    ...operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Constitutive Hybrid promoter...

  6. CRISPR Guide

    Type
    Guide
    ...created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine ...the DNA and replaces it with another base. In E. coli , this strategy yields C to A base edits, whereas...harnessed for antiviral mechanisms in bacteria ( E. coli ). Like the other Cas enzymes, the Cascade-Cas3 .... (2018). Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors....

  7. Molecular Cloning Techniques

    Type
    Guide
    ... The product may be transformed directly into E. coli , where the normal replication process will repair...
Showing: 1 - 7 of 7 results