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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6 results for: Coli

Showing: 1 - 6 of 6 results

  1. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different concentrations...antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of antibiotic makes it easy...solution in sterile water (or other antibiotic) E. coli transformed with a plasmid containing the carbenicillin...absorb. During the incubation, transform DH5α E. coli cells by heatshock with the plasmid of interest....transformation protocol. Plate 50 µL of transformed E. coli /rescue media suspension onto the agar and gently...Plate with No Carbenicillin Plate shows a lawn of E. coli and no selection. 150 µL of 0.1 mg/mL Carbenicillin...Carbenicillin plated over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...of CRISPR pooled plasmid libraries in Escherichia coli cells....amplification of a pooled-plasmid library in Escherichia coli cells. Pooled libraries contain tens to millions...Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not...

  5. General Transfection

    Type
    Protocol
    ...also be propagated in an endonuclease negative E. coli strain such as NEB stable. Dilute 1:3 (µg DNA:µg...

  6. Lentivirus Production

    Type
    Protocol
    ...also be propagated in an endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total...
Showing: 1 - 6 of 6 results