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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 625 results for: EMB

Showing: 61 - 80 of 625 results

  1. CRISPR References and Information

    Type
    Collection
    ...collection of protocols, materials, and publications by members of the Division of Signaling and Functional Genomics...cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs design and... (pX330) Protocol at Addgene Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9...

  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCCTGGCACAGGGGCTctccaccatgctggccAACCTGTTCTCAATGA kinesin-family-member-13a TAL3106 & TAL3107 TGTTCTCTGTTTGAGCGAgtctccacacagcagaGCGACAGTAACAGCTTCA...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA...TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA matrixmetalloproteinase14a (membrane-inserted) TAL3486 & TAL3487 TGTTCCTGGTGGCGGTGCatgaattgggtcatgcaCTTGGTTTGGAGCACTCA...

  3. Bacterial Expression Systems

    Type
    Collection
    ...periplasmic space between the inner and outer membranes. Targeting proteins to the periplasm reduces bacterial... in plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain...Collection page to find plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium...

  4. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning...ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and higher level gene networks...

  5. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...on an agarose gel Watch the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial Transformation ... new window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...

  6. Antibody Production

    Type
    Collection
    ...encoded by mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(...cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...

  7. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to...pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A ...

  8. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T...AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin...

  9. AAV Production in HEK293 Cells

    Type
    Protocol
    ...drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store... pH to ~8.5 Filter sterilize through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start...supernatant as follows: Filter through a 0.45 μm PES membrane. Add 40% PEG such that the final PEG concentration...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ...Neurodegeneration Research Collection Addgene has assembled the following to help you find information and...cases having at least one other affected family member. These familial cases, usually inherited in an ...

  11. COVID-19 Resources

    Type
    Collection
    ...site for furin proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function.( 5 ) TACE - (ADAM17) a metalloprotease...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...commonly added to aid in protein purification. Just remember to remove the stop codon for C-terminal tags and...yeast expression vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - ...

  13. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...annealed oligos into pX330 using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction ...utilized to be able to perform PCR in step 6.3 . Assemble a 20 μl PCR with the following components: 10 ...

  14. Plan Your Experiment

    Type
    Collection
    ...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...

  15. General Transfection

    Type
    Protocol
    ...drifted. Filter the solution through a 0.22 µm membrane. Aliquot 500–1000 µL into sterile tubes. Store...limited effect on cell growth. Last reviewed on: November 7, 2023...

  16. Protocol - Bacterial Transformation

    Type
    Protocol
    ... still get enough colonies for your next step. Remember that each of these shortcuts will reduce the efficiency...field is applied to the cell/DNA mixture to induce membrane permeability. To do this you will need to have...

  17. Using a Light Microscope Protocol

    Type
    Protocol
    ...started in the lab! Introduction Microscopes are emblematic of biological research and are found in many ...focus. Pro-Tip As you increase your magnification, remember that the objectives are larger. Adjust your focus...

  18. Pipetting Protocol

    Type
    Protocol
    ...pipette tip and using the pipette. Last Update: September 2022 Video Watch the video for tips on pipetting...preloaded into tip boxes. In either case, once assembled into their boxes, pipette tips can be sterilized...

  20. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright Addgene 2006, All Rights Reserved... . 1996 272:263-267. PubMed . Asymmetry in the assembly of the RNAi enzyme complex. . Schwarz DS et. al...
Showing: 61 - 80 of 625 results