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Addgene

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We narrowed to 430 results for: ERG

Showing: 61 - 80 of 430 results

  1. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was collected...expression changes of more than 100,000 cells undergoing iEP conversion, the Morris Lab found that cells... to uncover two distinct trajectories of MEFs undergoing iEP conversion, but CellTag could be used to ...study other biological processes where cells are undergoing identity changes, such as the differentiation...

  2. Tags and Other Markers

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

  3. Neuroscience

    Type
    Collection
    ...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control...

  4. TREAT-AD Plasmid Collection

    Type
    Collection
    ...disease (AD) by generating validated resources for emerging targets in AD and creating high-quality, well-...

  6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...transposons, the Sternberg and Zhang labs developed CRISPR-transposons. The Sternberg lab's system called...and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different genetic systems...References Bikard D, Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014..., Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse...

  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...suddenly cool: DNA methylation. In 1983, Andrew Feinberg and Bert Vogelstein showed, for the first time...methylation detected in a human disease: cancer (Feinberg & Vogelstein 1983). The floodgates, as they say...methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization, this method...827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A, Vogelstein B (1983) Hypomethylation distinguishes...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Health Organization has declared a global health emergency and called for the fast-tracked development of... was underscored by the potential scale of the emerging outbreak, as many as 2.2 billion people globally...prototyping and sensor assembly pipeline for future emerging pathogens or health crises. Many thanks to our..., and self-assembly for applications in health, energy, and sustainability.   References 1. Pardee, Keith...

  9. Antibodies

    Type
    Collection
    ... are expressed from plasmids. These antibodies undergo application-specific validation and consistent ...

  10. KLF Research Plasmids

    Type
    Collection
    ...Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About ...

  11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... visible and infrared light to dissipate excess energy at wavelengths of low photosynthetic activity. ...lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4)...10.1074/jbc.m606921200 Dove SG, Takabayashi M, Hoegh-Guldberg O (1995) Isolation and Partial Characterization...48604-4 Salih A, Larkum A, Cox G, Kühl M, Hoegh-Guldberg O (2000) Fluorescent pigments in corals are photoprotective...

  13. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...fluorescent proteins (Gambarotto, et al. 2019; Tillberg, et al. 2016). That being said, the anchoring,.../doi.org/10.1371/journal.pbio.3001020  Chen F, Tillberg PW, Boyden ES (2015) Expansion microscopy. Science...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...101796. https://doi.org/10.1016/j.mex.2022.101796  Tillberg PW, Chen F, Piatkevich KD, et al (2016) Protein-retention...

  14. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...real goal. We are all constantly negotiating our energy and attention. The goal is to maximise the time...Check out Joanne's Reddit AMA Managing time and energy between work and the rest of life is sometimes ...will worry less about how they will handle an emergency and this will make you much more confident about...daughter vehemently reminded her doctor that she was allergic to penicillin – I am much less worried knowing...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...specific neuronal subtypes such as inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed... you to target and functionally manipulate GABA-ergic interneurons across species and will undoubtedly...You can find the AAV vectors for infecting GABA-ergic interneurons here. Dimidschstein J, et al. Nature... use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging...Under the supervision of Dr. Tan, a team of undergraduates created DNA ladders (aka DNA molecular weight...

  16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...update of this post. References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto...://doi.org/10.1186/s13059-018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung...nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...://doi.org/10.1126/science.aba8853 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...and expand our current knowledge. The Abudayyeh-Gootenberg lab (also called the AbuGoot lab), a joint lab...lab run by Omar Abudayyeh, PhD, and Jonathan Gootenberg, PhD, focuses exclusively on this space — developing...Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable RNA targeting with the single-protein...Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome insertion...

  18. All Antibodies

    Type
    Collection
    ...recombinant antibodies. These monoclonal antibodies undergo application-specific validation and quality control...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light at ...release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally ...FRET signal. FRET is a non-radiative transfer of energy from one fluorescent protein to a compatible fluorescent...other fluorescent probes but can still transfer energy to the fluorescent protein acceptor. NLuc is an...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...: 797459. Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322...frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009...and define recA-independent mechanisms. Avoid overgrowing the bacterial culture Another way to prevent ...
Showing: 61 - 80 of 430 results