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... in a new window) The Malate Dehydrogenase CUREs Community (MCC) (Link opens in a new window) develops...mentor future MCC members. Find a regional hub (Link opens in a new window) and contact the hub director...opens in a new window) — Learn how to get started with a CURE, explore MDH projects, find teaching resources...expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned... cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present ...plasmid page. Use the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human.... DOI: 10.24918/cs.2021.43 (Link opens in a new window) External Collaboration Results in Student Learning...

... a new window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , MIT ...new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window) . ...molecular tools for high-throughput reconstruction of individual neurons in brain tissue developed by scientists...tools enabling high-throughput reconstruction of individual neurons in brain tissue. PRISM begins with the... PRISM is part of an integrated pipeline, but individual components can be used separately for additional...also developed a set of Cre-dependent, hSyn-Tet inducible plasmids (Addgene #242782–242799) suitable for...Potential applications could also include using individual or subsets of barcode proteins to label and detect...

...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...directly after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded...CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in...protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy the...foreign DNA. Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...(tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9...

...Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top Cyan Plasmid...Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald Plasmid...Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top Orange Plasmid...Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...interest. Most of these plasmids are available to industry scientists as well as academic and nonprofit labs...Molecular Expressions website (Link opens in a new window) for images, tutorials, protocols, and more. Empty...

...38913-z (Link opens in a new window) PMID: 30783202 (Link opens in a new window) Lee, S., Lim, W. A., Thor....0067902 (Link opens in a new window) PMID: 23844123 (Link opens in a new window) Loqué, D., Lalonde, S., ...nature05579 (Link opens in a new window) PMID: 17293878 (Link opens in a new window) Sheff, M. A., Thorn, K. ....1130 (Link opens in a new window) PMID: 15197731 (Link opens in a new window) Vickers, C. E., Bydder, ...12-96 (Link opens in a new window) PMID: 24161108 (Link opens in a new window) Content last reviewed: 12...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...Systems Biology Lab website (Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt...

...CAG Alzheimer's Dennis Selkoe 30145 pCAX APP Swe/Ind APP CAG Alzheimer's Dennis Selkoe 30146 pCAX APP ...Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind APP CMV Alzheimer's Aleksandra Radenovic 114410 AICSDP...Huntington's Susan Lindquist 1178 p416 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1179 p416 72Q GPD...Huntington's Susan Lindquist 1180 p416 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1181 p426 25Q GPD...Huntington's Susan Lindquist 1182 p426 46Q GPD HTT GFP GPD Huntington's Susan Lindquist 1183 p426 72Q GPD...Huntington's Susan Lindquist 1184 p426 103Q GPD HTT GFP GPD Huntington's Susan Lindquist 1185 p416 25Q GAL...Huntington's Susan Lindquist 1186 p416 103Q GAL HTT GFP GAL1 Huntington's Susan Lindquist 1187 p426 25Q GAL...

...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here....Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation...Plasmid Description Additional Resources iNDI (Link opens in a new window) is an ongoing project of the Center...Collection The iPSC Neurodegenerative Disease Initiative (iNDI) project aims to generate and characterize single...and Related Dementias (CARD) (Link opens in a new window) at the NIH. Reference Ramos et al. 2021. Tackling...:1080-1083. PMID: 33831364 (Link opens in a new window)...

... new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window) National...(Link opens in a new window) NIH AIDS Reagent Program (Link opens in a new window) Saccharomyces Genome...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... viruses, parasites such as protozoa, and fungi. Find plasmids below for the species you work with, or...they cause. The standard format for providing individual plasmids is a bacterial stab (of a laboratory...expression, and synthetic biology. CRISPR Resources : Find essential background information on using CRISPR...for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB plasmid set : E. ...

...NotI (GC-rich recognition site) 165508 pOpen-HindIIIR HindIII 165526 pOpen-PstIR PstI 165528 pOpen-SfiIR...single-stranded DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579...Enzymes Additional Resources (Link opens in a new window) The Open Enzyme Collection consists of more than...protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused with other enzymes to...functionality. The Open Bioeconomy Lab (Link opens in a new window) designed and codon-optimized the Open Enzymes ...Lab's Open Enzyme Collection (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection...for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols for...

... a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular switches...cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene... proteins with multiple subunits or isoforms, individual links to each gene page are provided below. Color... Color is used for clarity and does not indicate a specific relationship. The content and map for this...Research Color is used for clarity and does not indicate a specific relationship. The content and map for...to top Ras Pathway - Gene List Click on a name to find available plasmids for the gene. For components ...components with multiple subunits or isoforms, individual links to each gene page are provided below. Symbol Name...

...BsaI + HindIII No Caixia Gao 172716 pPEgRNA Bacteria J23119 (BBa_J23119) pegRNA SpeI + HindIII No Tilmann...Find prime editor plasmids for (search and replace) genome editing. CRISPR...gRNA, the pegRNA is designed with a spacer that binds to a specific genomic DNA locus and directs the ... site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template...non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the pegRNA and the desired edit is...optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease protection factor La to PEmax to enhance... pegRNA? Try the DeepPrime (Link opens in a new window) design tool from Hyongbum Kim's lab . Browse, ...

...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase ...opens in a new window) PHP.V1: Kumar et al., 2020 Pubmed 32313222 (Link opens in a new window) MaCPNS1, MaCPNS2...opens in a new window) AAV9-X1.1: Chen et al., 2023 Pubmed 37291094 (Link opens in a new window) Don’t See... the Caltech CLOVER Center (Link opens in a new window) . As part of our standard viral production , these... the Caltech CLOVER Center (Link opens in a new window) and was adapted from Challis et al. (2022). Text...repository of published capsid use (Link opens in a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These... LY6A ( Huang et al., 2019 (Link opens in a new window) ) to cross the blood brain barrier. For information...

...Anti-Calbindin [L109/39R] Calbindin Human Mouse IgG2a 177455 Anti-Calbindin [L109/57R] Calbindin Human...Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R] Neuregulin-HBD (Heparin binding domain, Type I/...Homer1 Mouse Mouse IgG2a 188187 Anti-Calbindin [L109/62R] Calbindin Human Mouse IgG2a 188188 Anti-Homer1... Mouse IgG2b 206635 Anti-CALB1/calbindin [L109/39R-2b] CALB1/calbindin Human Mouse IgG2b 206636 Anti-Homer1L...Human Mouse IgG1 206686 Anti-CALB1/calbindin [L109/39R-1] CALB1/calbindin Human Mouse IgG1 206687 Anti-Homer1L...Co-Rest/RCOR1 Human Mouse IgG2a 222146 Calbindin [L109/43R] Calbindin Human Mouse IgG2a 222147 Homer1 [L113... IgG2a 227042 Calbindin (Homo sapiens) recombinant mouse monoclonal antibody. Calbindin Human Chimera:...

...proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the gene...list below. For multiple isoforms or subunits, individual links to each gene page are provided below. Color... Color is used for clarity and does not indicate a specific relationship. The content for this page was...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ... top mTOR Pathway - Gene List Click on a name to find available plasmids for the gene. For components ...components with multiple isoforms or subunits, individual links to each gene page are provided below. mTORC1 Complex...4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin D1 Also known as CCND1 eIF-4E ...

... interactions, drug-induced changes, and more. NanoBit (Link opens in a new window) : The NanoLuc® enzyme... of LumiLuc (ATP-independent luciferase derived from teLuc) fused to mScarlet-I. Find more NanoLuc® tools...NanoLuc® is small (19 kDa), very bright, and ATP-independent, and a secreted version of the protein is available...William Kaelin 60495 pSBtet-GP Firefly TRE Dox-inducible expression of firefly luciferase Eric Kowarz 18782...Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc...luciferase reporter for? Browse or use the search box to find a construct that contains your regulatory element... form an active enzyme, generating luminescence. Find plasmids with LgBit and SmBit . Cluc-FKBP and FRB-Nluc...

...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and tetracycline... Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression of EYFP. 1 Viviana Gradinaru...transactivators that can be used with tetracycline (tet)-inducible expression systems . ID Name Expression System...tTA). 2 Viviana Gradinaru 99120 pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off...Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression...hSyn-fDIO-taCasp3-TEVp Syn-driven, Cre-dependent Inducing apoptotic ablation of neurons through a process...Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP)...

... in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...Nature review (Link opens in a new window) (Link opens in a new window) The Impact of Genomic Variation ...Function (IGVF) Consortium (Link opens in a new window) aims to understand how genomic variation affects...Resources The IGVF Consortium (Link opens in a new window) is funded by the National Human Genome Research...PubMed (Link opens in a new window) ....

...( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system that enables researchers... you first need to have a biological question in mind. Then you can decide how to answer that question... Cas9 exert their activity within a very narrow window proximal to the PAM sequence Base Edit Prime Edit...reverse transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be... choose from. Below are a few factors to keep in mind when designing and cloning your gRNAs. Select Genetic... editors exert their functions within a limited window from the PAM sequence, and base editor variants...variants often have different optimal editing windows. Be sure to refer to the literature associated with your...

...117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein...to generate AAV virus that will express light-inducible site-specific iCreV recombinase Hongkui Zeng 140135...to generate AAV virus that will express light-inducible site-specific iCreV recombinase Hongkui Zeng 140136... to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136... to generate AAV virus that will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137...to generate AAV virus that will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137...to generate AAV virus that will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552...

...expression levels of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed along...addresses", where dCas9 is going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression...first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the second binds to mcherry...modulating expression levels of two targets independently. Depositor...plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage...