...University of California, San Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations...

...the TREAT-AD plasmids that are currently available. ID Plasmid Gene/Insert Vector Type Backbone Mutations...

... other cell types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link...Initiative (iNDI) Collection You May Also Like... CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research...project aims to generate and characterize single nucleotide variant lines in an isogenic cellular background...

...Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

...phenotype and shares their plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional...

... also wish to join the TALengineering newsgroup . ID Plasmid 32250 TAL006 32251 TAL007 32253 TAL009 32254...

... domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat Binds 32285 JDS70...methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details...

...plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...packaged in AAV particles. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

...Armamentarium Project below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo...

...recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional...

...available for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1...be helpful to the scientific community. Penn ID Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-...Controls To look up a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors...Penn Vector Core and Addgene partnership to provide AAV viral preps, includes listing of transfer status...Penn Vector Core) and Addgene have partnered to provide high-quality AAV vectors to the academic research...

...with 2nd and 3rd generation lentivirus technologies. ID Plasmid Generation Type Description PI 12253 pRSV-Rev...CRISPR Empty gRNA plasmids table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro...Lentiviral Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety Blog: Tips...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these...increased researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components...infectious viral particle (a virion) are generally divided among multiple plasmids. Some plasmids (typically...transfer plasmids," which can be modified to encode a wide range of gene products. The main restrictions on...

...target sites throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid GenBank Key Features 26092 p15TV-L EF456736...

... Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype...based on our in-house testing and data provided by trusted outside labs – with full experimental details...accessibility in research. For each antibody, we provide important details including the protein and plasmid...about antibodies and their use, visit our Antibody Guide . Browse or search the table below or visit the ...you choose, store, or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody...

...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...was originally used. Here are a few questions to consider when choosing a gRNA: Does a given gRNA sequence...the deposit process. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...enzyme activity, PAM requirements, experimental considerations, and more, created and maintained by the Doudna...

...sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality ... about Addgene's Beta Projects and how you can provide us with input and feedback on this service. DNA...and Customer Support Teams are also available to provide assistance at [email protected] . Don’t See What...

...target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers ...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced... a new window) is available to enable users to identify both dimeric TALEN and monomeric TALE transcriptional...additional pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual...

...transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core catalog items. Virus-associated... experimental conditions. Protocols Written and video protocols for how to use your viral prep and how... Addgene, with details about storage. Biosafety Guide Documentation for your institutional biosafety office...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...used to make the virus. In order for Addgene to provide viral vector services to you, we must ensure that...

...allow you to insert and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR...expresses the transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti...expression. Several endogenous promoters had been identified that responded to stimuli, such as hormones or... lac system with the inducer isopropylthio-β-galactoside (IPTG) was tested first, but IPTG was found to... Element (TRE) A TRE is seven repeats of a 19 nucleotide tetracycline operator (tetO) sequence, and is...domain, resulting in reduced gene expression. This idea of a hybrid transactivator was initially used with...1995, Gossen et al. used random mutagenesis to identify which amino acid residues of tetR were important...