We narrowed to 497 results for: LARGE

...entire axial volume of a sample. Therefore, when a large number of optical slices are acquired all fluorophores...they require both optical sectioning, imaging over large distances and high temporal resolution. Traditionally...photobleaching when acquiring multiple images over a large axial range. 2-photon imaging, which restricts excitation....0NA objective with proper sampling. Therefore, large, cleared tissues need to be imaged via Lightsheet...this size, those that can will completely image large tissues in a few hours. Although the modern explosion...

...vertical.  Large central box. Another commonly used and effective style is one employing a large central ...is written across the center of the poster in a large font. Hence, spectators can read the text from many...written and graphical information are inserted into larger circles or ovals, that are arranged in some logical...

... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...population distinct from cellular debris and other larger cells. Note, we don’t mind the warning message ...

..., the Rat Genome Sequencing Project conducted a large scale sequencing and concluded that nearly all disease-linked...relatively simple to maintain and breed and they have large, easily modifiable embryos, which is ideal for observing...attractive features are their eggs. Frog eggs are large, opaque, and produced in abundance, making them ...make an ideal model organism: quick development, a large number of offspring (produces many seeds), self ...disease mode (Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies...

...short life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit... small, easily maintained as they are housed in large groups, easily bred, and produce 50-300 eggs at ...transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans are also...self-fertile hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly...

...- mK+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...

...targeting constructs. In the example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the ... the cells that have randomly integrated all or large portions of the plasmid. Deliver your construct ...CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult. Addgene depositors...

...strains, including HB101 and derivatives, release large amounts of carbohydrates that can inhibit the cell...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around 220 RPM,...coli strains. Increase the volume Starting with a larger culture volume can improve plasmid yield. Our lab...

...is often the case with large genes. This is due to inefficient packaging of large inserts; a study by Kumar...start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt for... guides) while a single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example...dishes or increasing the vessel size. Opting for a larger vessel, such as a 3 or 5-layer flask will require...calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and adjusting the cell...

...change in genotype. Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling ...Epigenetic changes have been discovered to play a large role in a variety of diseases, ranging from cancer...changes in their DNA methylation patterns, either large increases or decreases. Hypermethylation of CpG ...

... to take next steps and follow up especially in large companies. It is fine to address the cover letter...might be able to help you. If the organization is large, you will need to be more directed and target someone...

...United States, I look forward to gathering around a large table for a meal with my family. I am thankful for...things, and one is that I get to gather around a large table to eat with my Addgene family every day. Do...

...removing the full ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for ... same time (Liu et al. 2018). However, removing large lncRNA fragments with multiple gRNAs poses the risk...Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in: insertion of a synthetic...

...paper and then another figure in another paper. A large fraction of readers cover the same ground again ...allows you to make meaningful collections from a large section of open access life sciences data. You can...

...researchers and ready access to stably archived large-scale datasets by computational biologists. ...genome-wide datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it ...

...his team created with the research community at large, "[Fluorescent proteins are] a whole new way of ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

...III-E, and then used this information to identify a large variety of related bacteria with similar Cas systems...fused integrase would then integrate into with a large DNA insert – or Programmable Addition via Site-specific...programmed for new genes. In comparison with other large insert techniques like HITI or HDR, which require... and adenovirus templates. The system, which is larger than Cas9, can also be used with mRNA and a synthetic...

...Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these two requirements...interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing...

... in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both...the clinic Since lentiviral vectors can deliver a large amount of DNA (~8 kb) with a relatively low immune...lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for ...Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel B, Socie G, Chrétien...

... SPH mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators...activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented...delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was...