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We narrowed to 492 results for: LARGE

Showing: 61 - 80 of 492 results

  1. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...vertical.  Large central box. Another commonly used and effective style is one employing a large central ...is written across the center of the poster in a large font. Hence, spectators can read the text from many...written and graphical information are inserted into larger circles or ovals, that are arranged in some logical...

  2. Five Popular Model Organisms

    Type
    Blog Post
    ...short life cycle of 8 to 14 days, and produce a large number of offspring that are laid externally. Fruit... small, easily maintained as they are housed in large groups, easily bred, and produce 50-300 eggs at ...transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans are also...self-fertile hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ..., the Rat Genome Sequencing Project conducted a large scale sequencing and concluded that nearly all disease-linked...relatively simple to maintain and breed and they have large, easily modifiable embryos, which is ideal for observing...attractive features are their eggs. Frog eggs are large, opaque, and produced in abundance, making them ...make an ideal model organism: quick development, a large number of offspring (produces many seeds), self ...disease mode (Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...- mK+), λ– EPI300   Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on the...forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on the cell’s size...cell population will show up on an FSC/SSC plot, largely depends on cell type and laser voltage. The voltage...populations displayed. As the separation pattern largely stays the same and does not impact my ability to...population distinct from cellular debris and other larger cells. Note, we don’t mind the warning message ...

  6. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...targeting constructs. In the example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the ... the cells that have randomly integrated all or large portions of the plasmid. Deliver your construct ...CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult. Addgene depositors...

  7. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...is often the case with large genes. This is due to inefficient packaging of large inserts; a study by Kumar...start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt for... guides) while a single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example...dishes or increasing the vessel size. Opting for a larger vessel, such as a 3 or 5-layer flask will require...calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and adjusting the cell...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...strains, including HB101 and derivatives, release large amounts of carbohydrates that can inhibit the cell...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around 220 RPM,...coli strains. Increase the volume Starting with a larger culture volume can improve plasmid yield. Our lab...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...change in genotype. Epigenetic modifications play a large role in turning genes ‘off’ and ‘on’, signaling ...Epigenetic changes have been discovered to play a large role in a variety of diseases, ranging from cancer...changes in their DNA methylation patterns, either large increases or decreases. Hypermethylation of CpG ...

  10. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ... to take next steps and follow up especially in large companies. It is fine to address the cover letter...might be able to help you. If the organization is large, you will need to be more directed and target someone...

  11. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...United States, I look forward to gathering around a large table for a meal with my family. I am thankful for...things, and one is that I get to gather around a large table to eat with my Addgene family every day. Do...

  12. Retrovirus Plasmids

    Type
    Collection
    ...LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...removing the full ncRNA locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for ... same time (Liu et al. 2018). However, removing large lncRNA fragments with multiple gRNAs poses the risk...Mutations of splice sites. (C) Removal of an exon or a large genomic fragment. (D) Knock-in: insertion of a synthetic...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... SPH mice CRISPRa has largely been limited to in vitro settings due to the large size of Cas9 activators...activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented...delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was...

  18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

  19. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...Additionally, CAPTURE 3C-based sequencing requires a large number of cells (~5x107). Together, these two requirements...interactions identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with...chromatin interaction networks since they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...III-E, and then used this information to identify a large variety of related bacteria with similar Cas systems...fused integrase would then integrate into with a large DNA insert – or Programmable Addition via Site-specific...programmed for new genes. In comparison with other large insert techniques like HITI or HDR, which require... and adenovirus templates. The system, which is larger than Cas9, can also be used with mRNA and a synthetic...
Showing: 61 - 80 of 492 results