We narrowed to 1,015 results for: LEA
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TypeCollection...mice use LY6A to cross the blood-brain barrier, leading to strain-specific tropism (See S3 table (Huang...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER...using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru and cite as follows...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...
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CRISPR Pooled gRNA Libraries
TypeCollection...modify a single genomic locus. Before ordering, please review the steps for using pooled CRISPR libraries...Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species PI Lentiviral...Weissman 3rd 6 Varies Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout ...available items found to match all requested criteria. Please consider using fewer filtering options. Browse ... -
Cre-lox system
TypeCollection...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...Zeng 140135 pAAV-EF1a-iCreV Split Cre fused to nuclear localized wild-type VVD EF-1 alpha AAV Zeng 140284...expression of Cre Mouse Sncg AAV Hu 154018 LiOn-CMV∞Cre leak-proof iOn (LiOn) Cre switch CMV Mammalian Livet ...containing FLEx technology. Read the genOway press release for more information. Empty backbones to regulate...24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP... -
CRISPR Plasmids - gRNAs
TypeCollection...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published... -
Retrovirus Plasmids
TypeCollection...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ... -
Chemogenetics AAV Preps
TypeCollection...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging... -
CRISPR Plasmids - Prime Edit
TypeCollection..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA... -
Plant Plasmids and Resources
TypeCollection...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology... -
Lentiviral Prep Service
TypeCollection...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but... -
Zebrafish Plasmid Collection
TypeCollection...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR... -
Brain Armamentarium
TypeCollection...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan... -
Tetracycline Inducible Expression
TypeCollection...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...dox) is present, TetR binds to tetracycline and releases tet O, enabling transcription (Figure 1). While...tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how... -
Plasmids for Stem Cell Research
TypeCollection...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into... -
Biosensor AAV Preps
TypeCollection...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging... -
TREAT-AD Plasmid Collection
TypeCollection...Consortium (SGC) Plasmids Haian Fu Lab plasmids Opher Gileadi Lab plasmids (Link opens in a new window) The TREAT-AD...AD and creating high-quality, well-characterized lead molecules for prioritized targets that are valued... -
Zinc Finger Consortium Reagents
TypeCollection... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions... -
TALEN Expression Vectors
TypeCollection...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pSB700 64046 Mammalian/Lentiviral none S. pyogenes Cerulean Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian...multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate...plasmid for the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid... -
Fluorescent Protein Guide
TypeCollection...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ...fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent ... -
Institute for Protein Innovation
TypeCollection...well-characterized antibodies in an open science model. Learn more about IPI (Link opens in a new window) Glypican...DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody Collection...