We narrowed to 1,017 results for: LEA
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TypeCollection...designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information...and maintained by the Doudna Lab . gRNA Plasmids Please note that the following table lists both published...
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Retrovirus Plasmids
TypeCollection...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...enter the nucleus during mitotic breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate...integrate randomly into the host's genome, which can lead to oncogenesis (termed insertional mutagenesis). ... -
Chemogenetics AAV Preps
TypeCollection...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging... -
CRISPR Plasmids - Prime Edit
TypeCollection..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA... -
Plant Plasmids and Resources
TypeCollection...biology - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for...Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in Plant Biology... -
Lentiviral Prep Service
TypeCollection...Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate double-stranded breaks ( DSBs...Suggestions Don’t see what you are looking for? Please let us know what you would like to see available... Suggestion Form (Link opens in a new window) . Please note this does not guarantee viral service, but... -
Zebrafish Plasmid Collection
TypeCollection...site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make...Highlights of Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR... -
Brain Armamentarium
TypeCollection...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan... -
Plasmids for Stem Cell Research
TypeCollection...plasmids The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun...and Sox2 Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental Potential of iPSCs. Cell Stem...Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion into... -
Tetracycline Inducible Expression
TypeCollection...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...dox) is present, TetR binds to tetracycline and releases tet O, enabling transcription (Figure 1). While...tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how... -
Biosensor AAV Preps
TypeCollection...Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype...available items found to match all requested criteria. Please consider using fewer filtering options. *AAVrg ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging... -
TREAT-AD Plasmid Collection
TypeCollection...Consortium (SGC) Plasmids Haian Fu Lab plasmids Opher Gileadi Lab plasmids (Link opens in a new window) The TREAT-AD...AD and creating high-quality, well-characterized lead molecules for prioritized targets that are valued... -
Zinc Finger Consortium Reagents
TypeCollection... method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease fusions... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pSB700 64046 Mammalian/Lentiviral none S. pyogenes Cerulean Church LRG (Lenti_sgRNA_EFS_GFP) 65656 Mammalian...multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate...plasmid for the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid... -
TALEN Expression Vectors
TypeCollection...these vectors also have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The...Assembly TALEN Kit . Vectors encoding wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ... -
Fluorescent Protein Guide
TypeCollection...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ...fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent ... -
Institute for Protein Innovation
TypeCollection...well-characterized antibodies in an open science model. Learn more about IPI (Link opens in a new window) Glypican...DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags and Other Markers Antibody Collection... -
Chemogenetics Plasmids
TypeCollection...Learn more about chemogenetic resources available at Addgene. See our inventory of chemogenetic constructs...and how to use these tools in your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids... -
CRISPR Plasmids and Resources
TypeCollection...continously developing and expanding. Addgene works with leading scientists in the field to ensure our CRISPR collections...links to CRISPR forums, and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune ... -
Serotype Testing AAV
TypeCollection... . Note: For a list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID ...would like to see available in viral prep format. Please note this does not guarantee viral vector packaging...