...the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral ...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for genome-wide...

...sequence viewer software and extensive feature library, our updated plasmid and sequence displays are ...features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies a variety...in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be...

...N-acetylglucosamine strain library construction in 8 days. To build an equivalent strain library, our pEcCas/pEcgRNA...N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained...screening markers. Capability to construct a strain library with different gene dosages to facilitate swift...

...-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https:...example, can be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools...structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding...

...research, pooled CRISPR lentiirus libraries, pooled barcoding libraries, and more. Download the green virus...

...files of research articles. It’s your own digital library for the full-text version of useful papers. For...options for helping you manage your own digital library. Check out Papers and Mendeley – these programs...options for searching and sorting your own digital library as well as some integration with internet-wide ...

...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product from the...

...Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids... Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources: CRISPR Plasmids...

.... (2018) generated an open reading frame (ORF) library to screen PPIs among 450 transcription factors ...flies. Although BiFC open-reading frame (ORF) libraries have been generated for yeast (Kim et al., 2019... (2018) Generation of a versatile BiFC ORFeome library for analyzing protein–protein interactions in live...

.... Diversity is introduced into the cap plasmid library by inserting short DNA sequences that contain all...amplification using pre-designed primers. By using this library to infect an animal expressing Cre in a cell type... to reach the brain. Delivering the CREATE AAV library via IV screens for capsids that are able to cross...

...difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications like lineage...PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang...Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about...

...Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the high-resolution tracking...

...created mTFP1 by transforming E. coli with a gene library of approximately 484 variants, including some potential... potential tetramer-breaking mutations. The library was then screened through successive rounds of mutagenesis...

...the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes...to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse...

...shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments...experiments. Get the full details on how to make these libraries from the post and the associated publication. ...

...including plasmids, ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA!     This is just...

...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate ...

...In VAMP-seq, the abundance of a protein variant library is monitored by fusing the variants to eGFP followed...reduced eGFP fluorescence. Cells expressing the library are then sorted by fluorescence-activated cell ...

... has been working on developing CRISPR pooled libraries for use in genetic screens and has contributed...Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members of the Addgene Advisory...

...for us to reach everyone. Therefore, to make our library more useful to our community, we’re asking you ...