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We narrowed to 236 results for: Library

Showing: 61 - 80 of 236 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...thetargeting sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry...the Broad’s Genomic Perturbation Platform Avana library (used by the Cancer Dependency Map) than lines ... Genetic Perturbation Platform to design a new library that is agnostic to ancestry which will reduce ... screens using the same reference genomes and libraries. Further complicating the issue is the long and...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...choice. For a genome-wide library to conduct genetic screens, however, a library composed of the latter ...hundreds of genetic screens using genome-wide libraries have shown high concordance between different ...Learn How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources...Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ...

  3. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...sequence viewer software and extensive feature library, our updated plasmid and sequence displays are ...features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies a variety...in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral ...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for genome-wide...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...N-acetylglucosamine strain library construction in 8 days. To build an equivalent strain library, our pEcCas/pEcgRNA...N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained...screening markers. Capability to construct a strain library with different gene dosages to facilitate swift...

  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https:...example, can be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools...structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding...

  8. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product from the...

  9. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...files of research articles. It’s your own digital library for the full-text version of useful papers. For...options for helping you manage your own digital library. Check out Papers and Mendeley – these programs...options for searching and sorting your own digital library as well as some integration with internet-wide ...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids... Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources: CRISPR Plasmids...

  11. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    .... Diversity is introduced into the cap plasmid library by inserting short DNA sequences that contain all...amplification using pre-designed primers. By using this library to infect an animal expressing Cre in a cell type... to reach the brain. Delivering the CREATE AAV library via IV screens for capsids that are able to cross...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications like lineage...PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang...Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...the past couple months: A new lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes...to wild type. A new genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse...

  17. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...shows how you can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments...experiments. Get the full details on how to make these libraries from the post and the associated publication. ...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    ...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate the PCR product Isolate ...
Showing: 61 - 80 of 236 results