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We narrowed to 222 results for: Library

Showing: 61 - 80 of 222 results

  1. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...thetargeting sequences of guides across 8 CRISPR libraries. Samples are divided into those of African ancestry...the Broad’s Genomic Perturbation Platform Avana library (used by the Cancer Dependency Map) than lines ... Genetic Perturbation Platform to design a new library that is agnostic to ancestry which will reduce ... screens using the same reference genomes and libraries. Further complicating the issue is the long and...

  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...choice. For a genome-wide library to conduct genetic screens, however, a library composed of the latter ...hundreds of genetic screens using genome-wide libraries have shown high concordance between different ...Learn How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources...Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...about each library can be found on the individual library page. Find Pooled Libraries of Interest...pneumoniae Veening N/A 1 1,499 MusCK Library 174196 (Library A) 174197 (Library B) 1000000178 (A & B) Knockout... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function...amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation...experiment, the next step is to pick a library. The pooled CRISPR libraries in Addgene’s collection differ based...a given CRISPR library are unique to the genome of particular organism, and the library is only compatible...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...

  4. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...sequence viewer software and extensive feature library, our updated plasmid and sequence displays are ...features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies a variety...in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be...

  5. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral ...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for genome-wide...

  6. Lentiviral Prep Service

    Type
    Collection
    ...knockout pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone...In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses...Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved...Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178 ...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting...Root Human activation pooled libraries 92379 Human CRISPRa sgRNA library Calabrese in backbone XPR_502...

  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...N-acetylglucosamine strain library construction in 8 days. To build an equivalent strain library, our pEcCas/pEcgRNA...N-acetylglucosamine biosynthesis, we constructed a strain library with 1-11 copies of integrated genes. We obtained...screening markers. Capability to construct a strain library with different gene dosages to facilitate swift...

  8. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...-Cas9 genome engineering parameters via a library-on-library approach. Nat Methods 12:823–826 . https:...example, can be made easily and cheaply using oligo library synthesis. Prior to Cas9 activators, similar tools...structure and function (Pan et al., 2018). cDNA libraries, which consist of plasmids that over-express coding...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Pooled Library 111708 Mode #2 Library (NEDD4-2 WW2 domain) Pooled Library 111707 Mode #2 Library (NEDD4...domain) Pooled Library 111706 Mode #2 Library (14-3-3σ) Pooled Library 111705 Mode #2 Library (14-3-3β) Pooled...serine-oriented library/kinase-library reactions (SERIOHL-KILR, Barber et al., 2018). Figure 3: The library of ~110,000...site library (Pooled Library #188536) into 10 subpools of ~11,000 phosphosites ( Pooled Libraries #188526...Pooled Library 188534 iSPI_pSer_Subpool#9 Pooled Library 188533 iSPI_pSer_Subpool#8 Pooled Library 188532...Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188529...Pooled Library 188528 iSPI_pSer_Subpool#3 Pooled Library 188527 iSPI_pSer_Subpool#2 Pooled Library 188526...

  11. Resolute Plasmid Collection

    Type
    Collection
    ...Insert Industry PI Pooled Libraries CRISPR and overexpression pooled libraries are useful tools for genetic...experiments. These libraries target the solute carrier family of proteins. Library ID PI Description Human... Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...proteins. Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins...proteins. Mouse SLC Knockout Library 141436 Superti-Furga CRISPR knockout library targeting mouse SLC proteins... Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human SLC proteins...Transporter Knockout Libraries 213695 221409 221410 Superti-Furga CRISPR knockout library targeting human ...

  12. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...plasmid DNA (rather than from genomic DNA or a cDNA library), roughly 18-21bp is usually sufficient to give...you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR. Isolate your PCR product from the...

  13. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...files of research articles. It’s your own digital library for the full-text version of useful papers. For...options for helping you manage your own digital library. Check out Papers and Mendeley – these programs...options for searching and sorting your own digital library as well as some integration with internet-wide ...

  14. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled Library The MORF library is a...Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is a comprehensive collection of human...identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218 Zhang... vectors. The individual clones, or the pooled library containing the entire pooled collection, can be...isoform in pooled screens. This collection and library can be used for screening transcription factors...

  15. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids... Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources: CRISPR Plasmids...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    .... Diversity is introduced into the cap plasmid library by inserting short DNA sequences that contain all...amplification using pre-designed primers. By using this library to infect an animal expressing Cre in a cell type... to reach the brain. Delivering the CREATE AAV library via IV screens for capsids that are able to cross...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...difficult-to-transfect cells. Lentiviral barcoding libraries have been used for complex applications like lineage...PMC4273606. 4. Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang...Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about...

  19. Cancer Research Plasmids and Resources

    Type
    Collection
    ...oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier Lab RAS Pathway Clone...and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root labs for characterization...and tumor suppressor. Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator Plasmids...Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance...
Showing: 61 - 80 of 222 results