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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 770 results for: Mos;

Showing: 61 - 80 of 770 results

  2. CRISPR Plasmids - Plants

    Type
    Collection
    ...homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower...

  3. Open Enzyme Collection

    Type
    Collection
    ...DNA-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579 pOpen-dromedNK...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that...

  7. Lentivirus Plasmids

    Type
    Collection
    ...Lentiviruses, a subset of retroviruses, are some of the most common and useful types of viruses used in research...

  8. CRISPR Plasmids - Tagging

    Type
    Collection
    ...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for...

  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCTTTCACGCATTGCACtacacattggcaagatgGCAGCCACCATCGGGAGA prothymosin alpha a TAL3344 & TAL3345 TTATCATTCGCATCTCGTatttctctttatattaTTTTATTCCGAGACCCCA...

  11. Caltech Systemic Capsids

    Type
    Collection
    ...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ... can be measured by the luminescence output. The most popular types of luciferase found in Addgene's collection...

  13. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...gently spread over the surface until the liquid is mostly absorbed (there is a very small visible volume ...gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in...

  14. Zhang Lab CRISPR Page

    Type
    Collection
    ... (SpCas9) nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single...

  15. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...following amendments: Notes: Gel purification is most efficient with lower % agarose gels, so you will...will want to isolate the DNA from the gel. This is most commonly done with a commercial gel purification...

  16. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...they recognize, restriction digests have become the most widely used method scientists employ to selectively...cut the plasmid into 3-8 pieces such that all (or most) fragments are small enough to be accurately sized...

  17. Centrifugation

    Type
    Protocol
    ...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

  18. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...number of cells is limited (e.g. primary cells). Most CRISPR libraries require the use of lentivirus to...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...
Showing: 61 - 80 of 770 results