We narrowed to 117 results for: PEP;

1. Plasmids 101: Modular Cloning

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   Blog Post

   ...potential. For example, the best promoter for a given peptide can easily be tested by generating Level 1 TUs ...

2. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions. ...

3. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   .../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic...

4. Open Resources and Plasmid Tools For Studying C. elegans

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   ...self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the split ...

5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ...through direct evolution, capsid shuffling, and peptide displays. These capsids will be selected both for...

6. Cloning Mammalian Cells with the Agarose Method

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   ...Baltimore. She is particularly interested in signaling peptide maturation in neural and endocrine cells. She can...

7. 3D Printing Meets CRISPR Cas9

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   ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

8. Which Fluorescent Protein Should I Use?

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   ...molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov et. al. (2010) “Fluorescent...

9. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   ...promise that we could instead have things like peptide or RNA aptamers that act as genetically encoded...

10. Optogenetics Guide

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    Guide

    ...Improved light-inducible dimers; Bacterial SsrA peptide is embedded in the LOV2 domain (iLID plasmid), ... target protein is to be sequestered Lumitoxin Peptide toxin for blocking K + (Kv) channels fused to LOV2...allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk Blue-light...

11. Immunology Research Plasmids and Resources

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    Collection

    ...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide PI3CG, PI3K..., FGFB, HBGF-2 FPR1 formyl peptide receptor 1 FMLP, FPR FPR2 formyl peptide receptor 2 ALXR, FMLP-R-II...gonadotropin, beta polypeptide CGB3, hCGB CGB1 chorionic gonadotropin, beta polypeptide 1 - CGB2 chorionic... gonadotropin, beta polypeptide 2 - CGB5 chorionic gonadotropin, beta polypeptide 5 HCG, MGC119822 CGB7...

12. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post

    ...sample of wild type cells, purified protein, or a peptide. The positive control will confirm that the procedure...

13. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...library of AAV9 mutants that had a small randomized peptide loop inserted in the AAV9 capsid. This mutant library...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...their broad range of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells (even...

15. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

16. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...residue of a protein or knocking out a very small peptide. In these cases, you have little flexibility as...

17. Getting the Most from Your Lentiviral Transduction

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    ...PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma...

18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...PMC2784028. 3. Munro, Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned...

19. Pouring LB Agar Plates

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    Protocol

    ...powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride 12.0 g agar-...

20. Coomassie Purity Stain of Recombinant Antibodies

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    Protocol

    ...in the cell (e.g., failure to cleave the signal peptide) and may not be functional. This sample would fail...