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We narrowed to 232 results for: SPL;

Showing: 61 - 80 of 232 results

  1. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed...References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo...

  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...abundant and ethanol concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong...complexity of the system, researchers have used split GAL4 architecture or combined the system with Flp...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

  4. Brain Initiative Collection

    Type
    Collection
    ...promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing...monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-...

  5. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...negative effect on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence...cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions with multiple other ...

  6. Science as Art

    Type
    Blog Post
    ...Jurassic Technology in LA, where it is currently on display.   Many universities are now following the trend...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this...

  9. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...small (Rep52 and Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of ...often driven by the AAV p40 promoter. Alternative splicing and differing translational start sites generate...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook from...similar apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega...

  14. RNA Extraction Without A Kit

    Type
    Blog Post
    ...meant for isolating RNA from rat spleens in which they homogenized spleens in a guanidinium thiocyanate solution...

  15. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd...low and high-intensity blue light. Unlike other split-Cre systems which can take up to 24 hours to cut...

  16. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Mapping antibody-escape mutations with the yeast display library. From Starr et al Science., 2021. SARS-CoV... designed so that the SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...cells with the CRISPR library in vitro before transplanting them into mice. For in vivo screens, mice are...another option is to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You...

  18. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...vitro (see figure 1B in Terskikh et al.) and even displays predictable kinetics over 14 hours of expression... table: Fast-FT, Medium-FT, and Slow-FT. These display progressively longer blue fluorescence maturation...

  20. Pipetting Protocol

    Type
    Protocol
    ... expel liquid into the pipette tip, the volume display which shows the set volume for the the pipette,...section will help you understand how to read each display to dispense the correct amount of liquid reagent...), while 0.5 µL would read as 050. Each volume display on these pipettes will also have small tick marks...you will be rotating dials behind the numerical display or the top of the plunger of the pipette. Select...
Showing: 61 - 80 of 232 results