...Posts Eukaryotic parasites continue to cause substantial death and disease in the world. Parasites such...

...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...

...actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that activates...

...REAL-Fast assembly method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable...

...editing window near the PAM site. Uridine is subsequently converted to thymidine through base excision...

...transient transgene expression, as well as the substantial immune response induced by the viral capsid. ...

...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...

...to an antigen, a particular part of a foreign substance. In addition to being useful for our bodies to...

...sequence remains unchanged and available as a substrate for another round. Figure 1: Overview of prime...

...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid...

...CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute...

...expression of fluorescent TAL effectors to visualize subcellular positioning of target DNA sequences in living...

...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...

...enzyme digest (subcloning), including design and experimental procedures.... Protocols Subcloning Plasmid Cloning...Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like... Restriction Digest of Plasmid...out our Empty Backbone Reference . Background Subcloning by restriction digest is a commonly used lab ...YGOI is in a bacterial expression vector. Using subcloning, you can easily move YGOI into a mammalian expression...find two different restriction enzymes for your subcloning. It is also possible to use a single enzyme, ...

...precludes tTA binding to the tetO sequences and subsequent increase in expression by the activation domain...

...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ...

... of a substance in that liquid. In order to accurately measure the concentration of a substance based ...to determine the concentration of a particular substance in that liquid. Molecules absorb different wavelengths...need to know the wavelength of light that your substance maximally absorbs. In the case of nucleic acid...

...Select multiple colonies, mini-prep each one, and subject each clone to Sanger sequencing to characterize...

...Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection Blog...

...powder 96-well microtiter plate Chemiluminescence substrate Plastic wrap Primary antibody Secondary antibody...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...shaking platform. Prepare the chemiluminescence substrate by mixing 1:1 reagent A to reagent B. Gently incubate...