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Showing: 61 - 80 of 505 results

  1. Pouring LB Agar Plates

    Type
    Protocol
    ... g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...

  2. Western Blot

    Type
    Protocol
    ... a sonication step may be required for hard to extract proteins such as those in the nucleus. Incubate...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...

  3. Synthetic Biology - Overview

    Type
    Collection
    ...Synthetic Biology Articles - Peccoud lab social media tracker Synthetic Biology - Open access journal Do you ...

  4. Retrovirus Plasmids

    Type
    Collection
    ...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

  5. Worm Expression Resources

    Type
    Collection
    ...consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments...

  6. Qi Lab CRISPR Page

    Type
    Collection
    ... plasmid ( pdCas9_bacteria ) contains an anhydrotetracycline (aTc)-inducible dcas9 gene on a p15A vector...

  7. AAV Packaged on Request

    Type
    Collection
    ...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

  8. Arf GTPase Family

    Type
    Collection
    ...Spang, A., Dacks, J. B., & Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking...

  9. Lentivirus ddPCR Titration

    Type
    Protocol
    ... min at 100 x g . Gently aspirate supernatant. Extract genomic DNA according to the GeneJet Genomic DNA...used to transduce HEK293T cells. Genomic DNA is extracted from the target cells and assayed for integrated...

  11. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...p-bacteria Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify...

  12. COVID-19 Resources

    Type
    Collection
    ...Community at Protocols.io Addgene's Protocol for RNA Extraction Without A Kit References ACE2 - Andersen, KG,...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...

  16. Weighing Reagents Protocol

    Type
    Protocol
    ...Pro-Tip When you weigh out a reagent, you may leave trace amounts of it on the balance. Therefore, it’s best...

  17. Video Library

    Type
    Protocol
    ...cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ... milk going to help me extract DNA from agarose gel?!”   The DNA gel extraction with glass milk method... a similar workflow as a column-based plasmid extraction kit. First, you lyse the bacteria and denature...contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase. Finally...ready for use! Plasmid purification and DNA gel extraction with glass syringe filters Starting material:...fragments The plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly...protocol and guanidine isothiocyanate for the gel extraction protocol) disrupts the DNA’s hydrogen bonds with... more flexible than commercial kits.  DNA gel extraction with glass milk Starting material: agarose gel...
Showing: 61 - 80 of 505 results