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... from Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools These AAV encode tools that can be...

...precise delivery of genetic material across a wide range of tissue types with minimal immune response in ...

...examples of popular tools to use in zebrafish, ranging from optogenetics to tools for studying diseases...

... new window) — all Open Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the...

...digital PCR (ddPCR) titration protocol (modified from Wang et al. (2018) (Link opens in a new window) ). Briefly...

...window) — The initiative promotes interdisciplinary exchange, open technologies, and responsible innovation...

...pAAV-hIBA1a-GFP-miR124T hIBA1a GFP Constitutive 5 Chun-Li Zhang 20299 pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA...

... The Rinehart and Söll labs at Yale University changed the way researchers can explore important questions...

... development, regression with a loss of spoken language and hand skills, development of repetitive hand...

... Cre-dependent Flpo recombinase Recombinases Li Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre...

...important insights into gene function. These genetic changes also allow for finer control over protein expression...

...NES-Twitch-GR CamKII Twitch none Constitutive 2 Yiyang Gong Calcium Sensor: jRCaMP1 100846 pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40...

...heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived V L and V H domain PCR products...

.../gmab036 Zhang Y, Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y...Sichuan Normal University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic ...References and Resources References: Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S (2015) Multigene Editing in....1128/aem.04023-14 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system... Y, Tao R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases...contributed by guest bloggers, Yiwen Zhang, a Ph.D. candidate in the Yang Lab, Qi Li, a lecturer at Sichuan...following changes. In pCas9, we 1) replaced the promoter of gRNA-pMB1 with PrhaB, 2) changed the replicon...

...broad): somatic and visceral sensory ganglia, type II spiral ganglion neurons Mice, rats No (mechanism... 4d, Jang et al., 2023). When combined with a specific promoter, AAV-PHP.AX shows greater changes in specificity...Chen, X., Ravindra Kumar, S., Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj, C. M., Ngo, V... Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan, E. E., Huang, S.-F., Ravindra...Cummins, A., Chen, Y., Fan, Y., Zhang, Q., Sharma, J., Duan, Y., Wang, L., Feng, G., Chen, Y., Ip, N. ...D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman,...

....2011.06.019  Wang, H., Yang, H., Shivalila, C. S., Dawlaty, M. M., Cheng, A. W., Zhang, F., & Jaenisch...homology arms. The Zhang lab typically use PAGE purified long oligos. For large changes (>100 bp insertions...performing a genotyping assay (such as Sanger sequencing). See Wang et al. (2013) for a useful reference... Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering... E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang, F. (2013). Double nicking by RNA-Guided ...https://doi.org/10.1016/j.cell.2013.04.025  Yang, H., Wang, H., Shivalila, C. S., Cheng, A. W., Shi, L...with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists to ...

....2013.04.025 Zhang X, Liang P, Ding C, Zhang Z, Zhou J, Xie X, Huang R, Sun Y, Sun H, Zhang J, Xu Y, Songyang...10.1038/s41467-017-02706-7 Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R (2013) One-Step...new technology to engineer mouse models (Wang et al., 2013, Yang et al., 2013). Like everyone else in the... Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering...Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H (2016) Generating Mouse Models Using ...Dion SL, Kutny PM, Zhang Y, Cheng AW, Jillette NL, Malhotra A, Geurts AM, Chen Y-G, Wang H (2015) Efficient... https://doi.org/10.1534/genetics.115.176594 Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R...

...02050-w Yi, Z., Zhang, X., Wei, X., Li, J., Ren, J., Zhang, X., Zhang, Y., Tang, H., Chang, X., Yu, Y., ...01595-6 Ye, L., Zhao, D., Li, J., Wang, Y., Li, B., Yang, Y., Hou, X., Wang, H., Wei, Z., Liu, X., Li, Y.,...Hong, M., Luan, C., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa... Y., Zhou, X., Chang, C., Chen, G., Liu, W., Li, G., Fan, X., Sun, M., Miao, C., Huang, Q., Ma, Y., Yuan...Yuan, F., & Chang, X. (2024). Protein language models-assisted optimization of a uracil-N-glycosylase ... H., Li, G., Zhou, J., Yuan, Y., Zhang, H., Shi, L., Yao, X., & Yang, H. (2023). Programmable deaminase-free...https://doi.org/10.1093/nsr/nwad143 Tong, H., Wang, H., Wang, X., Liu, N., Li, G., Wu, D., Li, Y., Jin, ...

...the study of heritable changes in gene expression that do not involve any changes to DNA sequence (Epigenetics...modifications can result in phenotypic changes without any change in genotype. Epigenetic modifications...reduces the risk of dangerous DNA damage. If the way DNA is wrapped around histones changes, then gene expression...Epigenetic changes have been discovered to play a large role in a variety of diseases, ranging from cancer...epigenetic changes were statistically significantly associated with reduced life-span, but no changes in the... visualize changes in gene expression and may lead to the development of a whole new range of methods ...expression can change as well. The more frequently and tightly bound DNA is to histones, the more compacted...

... designing your overhang sets. But are those rules the best way to design GGA overhang sets, particularly...(1) don’t use the same overhang twice; (2) avoid palindromes; (3) no overhangs with the same three nucleotides...number of overhangs, three or four base-pair sequences, and exclude or include specific overhang sequences...2018, they published a paper profiling four base overhang ligation (Potapov et al., 2018), which showed ...two nucleotides in the same position; (5) avoid overhangs with either 0% or 100% GC.) In fact, the NEB® ...that high-fidelity reactions could be done using overhang sets that broke rules 3—5. Fidelity, it turns ...design high-fidelity Golden Gate Assemblies and overhang sets: NEBridge Ligase Fidelity Viewer™; NEBridge...