Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 648 results for: ato

Showing: 61 - 80 of 648 results

  1. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

  2. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani New viral...Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Gordon Fishell New viral prep pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based research as...of the gene, Cas9 activators efficiently solve these problems.   Which Cas9 activator should I use? There...significant work on constructing Cas9 transcriptional activators. These constructs allow for the upregulation ...genome-wide studies One of the best uses for Cas9 activators is in genetic screening. gRNAs targeting every... using oligo library synthesis. Prior to Cas9 activators, similar tools were made using other DNA binding...to easily change the sequence targeted by the activator by simply providing a new gRNA rather than engineering...organism, have been used in a similar manner to Cas9 activators. However, these can be difficult to construct...

  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Repressible: An activator protein is bound, turning transcription ON. When a repressor binds the activator protein...introduced the second generation QF2 activators. These activators solve the problem of broad QF expression-induced...protein, the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF....protein, the repressor protein can bind to the operator. The bound repressor then prevents transcription... repressor (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In... Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional...by Gossen and Bujard, both the promoter and transactivator components of the system have been engineered...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Dorus Gadella 37351 pQC membrane TdTomato IX Membrane Palmitoylation TdTomato Connie Cepko 22479 FUmGW Membrane...118737 pBOB-CARMIL2 BH domain-tdTomato Plasma Membrane CARMIL2 BH domain tdTomato John Cooper *Fusions to other...

  6. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...support educators interested in integrating MDH-related research into their teaching laboratories. They ...professional development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor...

  7. Recombinases AAV Preps

    Type
    Collection
    ... mCherry 1 Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn dTomato 1, 2, 5, 8, 9, rg*, PHPeB Larsen 107787 AAV.TBG.PI.Cre.rBGe...pAAV-EF1a-C-CreintG EF1a none 1 Cepko 69916 pAAV.cTNT.iCre cTnT tdTomato 9 Pu 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the Church...method, called STARR-seq (self-transcribing active regulatory region sequencing) that tests enhancer sequences...promoters, RBS’, coding sequences (CDS), and terminators of varying strengths. These can be easily combined...both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform (CCP); the latter of which ...pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are...and context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto ...effectiveness of their library design, Moffat laboratory members conducted multiple screens in different...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...the activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator domain...control transcription. Two separate labs, Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach...directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the figure below...and CIB1 proteins heterodimerize and move the activator into position to activate gene transcription. ...fusion of full-length CRY2 and the transcriptional activator domain VP64. Using this system in HEK293T cells...NHEJ and HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable system to cleave...DSBs (non detectable). Like their prior work, the Sato lab also showed that the paCas9-2 system could be...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of...Here's what you'll find in this post: Glutamate indicators with increased sensitivity and tailored deactivation...viral preps targeting interneurons Glutamate indicators with increased sensitivity and tailored deactivation...imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise..., Iyer, R., … Podgorski, K. (2025). Glutamate indicators with increased sensitivity and tailored deactivation...Glypicans By Ashley Waldron Glypicans are important modulators of cell signaling pathways during embryonic development...Shilatifard Lab was able to identify both new and known regulators at the FOS gene promoter and the MYC locus (Figure...

  11. Plant Plasmids and Resources

    Type
    Collection
    ...systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to... genomes using genome engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...Suntag” Cas9 activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each ...and engineered combinations of transcriptional activators to induce gene transcription at genomic sites... vitro settings due to the large size of Cas9 activators, which largely precludes delivery by adeno-associated...will fit). Cre-dependent expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by lentivirus, which...expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church Lab. Neuron-specific expression...

  13. Arf GTPase Family

    Type
    Collection
    ...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs....References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized as members..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...with examples of potential synthetic chromatin mediators and probes. Image from Priode et al., 2021. ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed by Rui Tang in Monte Winslow...label cells after cell-cell interactions. Using G-baToN, “sender” cells express GFP on the surface and “...receiver cell. Image from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration...lab recently "broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024...chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited gene to confer ...the team was able to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may... species. In addition, they generated offspring tomato plants that inherited the genomic edit but not ...prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513...2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic...

  17. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to...was creating a LEGO stage that resembles a real laboratory. It only took one night to build the first model...use our imaginations to mold it all into a full laboratory. It is priceless to see our “toys” gaining life...working as scientists in a custom made, colorful laboratory. When we started, the hardest part of the process...building each scenario, I go “WOW, now I have my own laboratory” (the main character is much more than a piece...

  18. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...Cancer Institute! Sonic Hedgehog, an important regulator of embryonic development The largest conference...the hedgehog signalling pathway, which is a key regulator of all bilateral animal development. SHH plays...publication, Ivics et al 1997. Spaghetti Squash, a regulator of cellular contraction You may think that a gene...vessels). Spaghetti squash (Sqh) is the Drosophila Regulatory Light Chain (RMLC) of nonmuscle myosin II (Karess... Jordan and Karess, 1997), which contains the regulatory regions that are necessary for myosin activity...monster, a meiotic arrest gene required for spermatogenesis One of our smaller conference rooms is named... meiotic arrest gene that is necessary for spermatogenesis. Flies that lack cookie monster are sterile...

  19. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...doi: 10.1038/s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR...PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators with improved activation kinetics and localization...have developed a new genetically-encoded voltage indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized...Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell. 2022 ...has generated a new genetically encoded calcium indicator using small monomeric NIR fluorescent proteins... of a Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi...they developed a family of fluorescent potassium indicators with a range of binding affinities (Torres Cabán...

  20. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...genes. We’ve created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together...CRISPR/Cas genetic circuits are linked to the regulatory networks within E. coli host cells. ...in building genetic circuits is getting enough regulatory proteins that don’t interfere with each other...programmable that you can make a very large number of regulators. That basically means larger genetic circuits...that could conceivably be as large as natural regulatory networks. Addgene: What kinds of things could... pattern, shape. All of that is controlled by regulatory networks in the plant. If we want to access that...CRISPR is how much easier it is to work with than regulatory proteins. There are still things we haven’t solved...
Showing: 61 - 80 of 648 results