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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 959 results for: cha

Showing: 61 - 80 of 959 results

  1. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... CUA. Engineered EF-Tu binds the phosphoserine-charged tRNA (AUC) and is brought to the ribosome where...incorporates phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein. Protein... The Rinehart and Söll labs at Yale University changed the way researchers can explore important questions...improved phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing...

  2. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... continue to optimize these tools. The best characterized SSR systems, Cre and Flp, are tyrosine recombinases... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  3. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR..., the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems are ...components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or ...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which ...

  5. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...of the C-terminal 18-21 amino acids and a D614G change which is present in many circulating variants. ...

  6. Microbiology Resources

    Type
    Collection
    ...Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

  7. Worm Expression Resources

    Type
    Collection
    ...hundreds of reporters have been developed and characterized which allow researchers to express desired constructs...Cas9, such as that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens...open-access collection of original, peer-reviewed chapters covering topics related to the biology of C. elegans...

  8. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates... equivalent to guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer...

  9. Open Enzyme Collection

    Type
    Collection
    ...technologies enabled by this collection include polymerase chain reaction (PCR), cloning, isothermal amplification...This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where...pOpen-9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...designed for rapid TALEN assembly. Kiran Musunuru and Chad Cowan Ekker Lab TALEN Kit All possible tetramers...Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used...49044 EMM71T Golden Gate TALEN 2.0 49401 pBlue-TAL Michal Zurovec pBlue-TAL was designed for use with the...

  11. Lentivirus Plasmids

    Type
    Collection
    ...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable...selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression ...

  12. Rett Syndrome

    Type
    Collection
    ... incidence of approximately 1:10,000. It is characterized by apparently normal development through the...differentiated cells that can be used to understand the mechanisms of the disease and for the development of successful... database of clinical features and genetic characteristics of Rett syndrome (Link opens in a new window...

  13. Depositor Collections

    Type
    Collection
    ...Recombinant mAb Plasmid Collection Disease Research Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative...Center TREAT-AD Plasmid Collection Visualization Michael Davidson Fluorescent Protein Collection Allen Institute...

  14. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... cell by non-homologous end joining (NHEJ); a mechanism which frequently causes insertions or deletions... used to faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication...

  15. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

  16. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...

  17. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...

  18. CRISPR Plasmids - Parasites

    Type
    Collection
    ... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...

  19. Institute for Protein Innovation

    Type
    Collection
    ...IPI is providing access to high-quality, well-characterized antibodies in an open science model. Learn more...Integrin Antibodies Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...

  20. Chemogenetics Plasmids

    Type
    Collection
    ...an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating or inhibitory depends... light-sensing optogenetic element such as channelrhodopsin. When the luciferase enzyme oxidizes its substrate...
Showing: 61 - 80 of 959 results