...Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...of the C-terminal 18-21 amino acids and a D614G change which is present in many circulating variants. ...

...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which ...

...Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

...hundreds of reporters have been developed and characterized which allow researchers to express desired constructs...Cas9, such as that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens...open-access collection of original, peer-reviewed chapters covering topics related to the biology of C. elegans...

... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates... equivalent to guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer...

...technologies enabled by this collection include polymerase chain reaction (PCR), cloning, isothermal amplification...This can help reduce lab costs and overcome supply chain issues, particularly in areas of the world where...pOpen-9N7polA (CT) 9 degrees N-7 DNA Polymerase chain terminating 165511 pOpen-K12polLF DNA Polymerase...

...designed for rapid TALEN assembly. Kiran Musunuru and Chad Cowan Ekker Lab TALEN Kit All possible tetramers...Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used...49044 EMM71T Golden Gate TALEN 2.0 49401 pBlue-TAL Michal Zurovec pBlue-TAL was designed for use with the...

...making Integrase deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and...selection. See plasmid 21916 for Neo selection. Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable...selection. See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression ...

... incidence of approximately 1:10,000. It is characterized by apparently normal development through the...differentiated cells that can be used to understand the mechanisms of the disease and for the development of successful... database of clinical features and genetic characteristics of Rett syndrome (Link opens in a new window...

... Photoactivatable , Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent...purified protein. Protein purification can be challenging, but once you get it right, you can learn many... Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based off of pBESTluc from Promega...

...Resources: FRET Biosensors Subcellular localization Michael Davidson Collection Blog: Which FP Should I Use...Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

...Recombinant mAb Plasmid Collection Disease Research Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative...Center TREAT-AD Plasmid Collection Visualization Michael Davidson Fluorescent Protein Collection - Davidson...

... cell by non-homologous end joining (NHEJ); a mechanism which frequently causes insertions or deletions... used to faithfully introduce specific genomic changes. ID Plasmid Gene/Insert Promoter PI Publication...

...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...

... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...

... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...

...IPI is providing access to high-quality, well-characterized antibodies in an open science model. Learn more...Integrin Antibodies Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...

... fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute...

... cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions...function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates...