We narrowed to 306 results for: construct

...selectable marker. This vector can express your construct in target cells transiently or constitutively ...Some customization is required, but many viral constructs have already been built and are available on ... embryonic stem cells. Viral integration of a construct is also reliably higher than traditional transfection...

...new gene construct incorporated randomly into the genome, knockin mice have the gene construct inserted...infection with a viral vector containing the gene construct can be used to insert the gene of interest into...

...microtubule severing. A) Schematic of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and ...is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes (the earlier...earlier article also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4)...100x variants. But those super-high efficiency constructs are great for in vitro biocatalytic applications...

...makes it easy to generate wild type and mutant constructs at the same time, rather than sequentially.   ...time it takes to get primers, you can have your construct made in 5 days. One drawback to the Gibson assembly...repair the nicks in vitro, whereas in SLIC these constructs are repaired in vivo, which ends up being much...PMC3684516. 5. Casini, Arturo, et al. "One-pot DNA construction for synthetic biology: the Modular Overlap-Directed...

...and efficient way to generate a wide variety of constructs. Similar, Gateway cloning (discussed below) can...enzymes. In Golden Gate cloning, the desired end construct does not contain the type IIS recognition sites...cloning (and be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun, et...

...2018). From this data, they generated lentiviral constructs using the High-Throughput Mutagenesis and Molecular...carrying the corresponding wild-type genes were constructed as well. They called this library the CTD2 FASMIC...

...   Here are some other practices to encourage constructive input from your team: Solicit feedback regularly...speaker accurately. Thank employees who volunteer constructive feedback and suggestions. Consider offering ...

...Plasmids for rapidly assembling plant transformation constructs, based on the Golden Gate method. Golden Gate... for hierarchical assembly of plant multigene constructs; contains yeast two-hybrid and bacterium-to-plant...High-performance vectors for creation of fusion constructs in transgenic analysis of plants. Return to top...MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9...Dicot Genome Editing Kit Plasmids to assemble constructs for genome editing in dicotyledonous plants. ...Toolkit A streamlined set of molecular tools for constructing CRISPR gene-editing vectors in just two simple...

...fused to the bacterial polymerase subunit ω. This construct is designed to activate transcription from the...xCas9 scaffold. With an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating...PAMs. xCas9 3.7 Activity in Mammalian Cells Construct NGG PAM NGA PAM NGC PAM NGT PAM GAA ...

...of viral constructs is a useful tool as it provides a means to quickly check that a construct is functional...making a stable cell line. When expressing a viral construct transiently care needs to be taken to ensure that...

...N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier ...on the same day.) This team created a similar construct that they called a Glycosylase Base Editor, or...PB) (Chen et al., 2021). Both of these fusion constructs improved editing in various cell types, although...efficiencies when fused to the N terminus of a GBE construct. This effect improved GBE editing but not CBE ...introduced the enzyme HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical ...editors by expression optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https...

...different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic...acoustic collapse to aid in the development of ARG constructs with new acoustic phenotypes. Want to start using...

...binding region RS20. When calcium is absent, the construct does not fluoresce; when present, calcium induces...check out our gene-specific biosensors. These constructs are often used to monitor enzymatic activity ...

...needs to identify cells containing the plasmid construct you’re seeking. Fortunately, there are many ways...sucrose medium will select for cells that contain constructs that have lost the sacB gene. SacB can be used...

...another way is to systematically offer thousands of constructs for tagged and untagged expression of SARS-CoV...Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire...

...Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems. Plasmids...certainly didn’t, but now we can with Cas13b/ADAR constructs from the REPAIR (RNA Editing for Programmable...

...percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin systems and their applications...Plasmid addiction systems can be great additions to constructs that are used to drive high protein expression...also used the hok/sok toxin-antitoxin system to construct a stable synthetic gene circuit that can be used...

... for the transgenic construct). More importantly, the expression of this construct is Cre-dependent, enabling...Figure 1: (A) Schematic of the Cre-dependent SPH construct for generating the SPH mouse. (B) AAV plasmids...

...receive an aliquot from the original library constructed by the depositing lab. (For our more popular ...library without an amplification protocol, or have constructed your own library, you’ll need to develop your...protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction, screening...

...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...introns Anthony Leung Return to Top Expression Constructs Luciferase driven by a strong constitutive promoter...luciferase). Feng Zhang Return to Top Reporter Constructs Already know what gene or regulatory element ...reporter for? Browse or use the search box to find a construct that contains your regulatory element of interest...rapamycin-inducible interaction. nLuc and cLuc : Constructs for split firefly luciferase complementation ...and Antares ) for in vivo imaging. NanoBRET : Constructs using NanoLuc® as a BRET energy donor and HaloTag...mScarlet-I. Find more NanoLuc® tools, including constructs for NanoBRET and NanoBiT assays, in the Promega...