...microtubule severing. A) Schematic of Opto-katanin constructs and activity. EB3N-VVDfast-iLID (anchor) and ...is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes (the earlier...earlier article also provided CIB constructs for targeting Golgi, ER, and endosome membranes) (Figure 4)...100x variants. But those super-high efficiency constructs are great for in vitro biocatalytic applications...

...makes it easy to generate wild type and mutant constructs at the same time, rather than sequentially.   ...time it takes to get primers, you can have your construct made in 5 days. One drawback to the Gibson assembly...repair the nicks in vitro, whereas in SLIC these constructs are repaired in vivo, which ends up being much...PMC3684516. 5. Casini, Arturo, et al. "One-pot DNA construction for synthetic biology: the Modular Overlap-Directed...

...and efficient way to generate a wide variety of constructs. Similar, Gateway cloning (discussed below) can...enzymes. In Golden Gate cloning, the desired end construct does not contain the type IIS recognition sites...cloning (and be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun, et...

...2018). From this data, they generated lentiviral constructs using the High-Throughput Mutagenesis and Molecular...carrying the corresponding wild-type genes were constructed as well. They called this library the CTD2 FASMIC...

...   Here are some other practices to encourage constructive input from your team: Solicit feedback regularly...speaker accurately. Thank employees who volunteer constructive feedback and suggestions. Consider offering ...

...Gradinaru 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213944-PHPeB pAAV_BiSSTe4_dTomato_nlsdTomato AAV construct expressing dTomato driven by SST interneuron-targeting...Gradinaru 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213940-PHPeB pAAV_BiPVe3_dTomato_nlsdTomato AAV construct expressing dTomato driven by PV+ basket cell-targeting...Gradinaru 213937-PHPeB pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...213936-PHPeB pAAV_BiPVe4_dTomato_nlsdTomato AAV construct expressing dTomato driven by chandelier cell-targeting... 213915-PHPeB pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin-2 driven...

...fused to the bacterial polymerase subunit ω. This construct is designed to activate transcription from the...xCas9 scaffold. With an NGG PAM, xCas9 3.7-VPR constructs modestly outperformed SpCas9-VPR in activating...PAMs. xCas9 3.7 Activity in Mammalian Cells Construct NGG PAM NGA PAM NGC PAM NGT PAM GAA ...

...of viral constructs is a useful tool as it provides a means to quickly check that a construct is functional...making a stable cell line. When expressing a viral construct transiently care needs to be taken to ensure that...

...N-glycosylase (UNG) from E. coli. The resulting construct, eUNG-BE4max(R33A)ΔUGI, was given the pithier ...on the same day.) This team created a similar construct that they called a Glycosylase Base Editor, or...PB) (Chen et al., 2021). Both of these fusion constructs improved editing in various cell types, although...efficiencies when fused to the N terminus of a GBE construct. This effect improved GBE editing but not CBE ...introduced the enzyme HMCES, either as a fusion construct or by co-transfection. HMCES forms a chemical ...editors by expression optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https...

...interactions and a series of FRET standards . Pre-constructed fluorescent biosensors targeting small molecules...also available. Custom biosensors can also be constructed using the cpFRET kit from the Pertz laboratory...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...via a 10 amino acid linker ACA Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Amber...linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa linker-Venus VCA...VCA Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Amber VCV Heterotrimeric...Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct...

...different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic...acoustic collapse to aid in the development of ARG constructs with new acoustic phenotypes. Want to start using...

...Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems. Plasmids...certainly didn’t, but now we can with Cas13b/ADAR constructs from the REPAIR (RNA Editing for Programmable...

...binding region RS20. When calcium is absent, the construct does not fluoresce; when present, calcium induces...check out our gene-specific biosensors. These constructs are often used to monitor enzymatic activity ...

...another way is to systematically offer thousands of constructs for tagged and untagged expression of SARS-CoV...Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire...

...needs to identify cells containing the plasmid construct you’re seeking. Fortunately, there are many ways...sucrose medium will select for cells that contain constructs that have lost the sacB gene. SacB can be used...

... for the transgenic construct). More importantly, the expression of this construct is Cre-dependent, enabling...Figure 1: (A) Schematic of the Cre-dependent SPH construct for generating the SPH mouse. (B) AAV plasmids...

...percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin systems and their applications...Plasmid addiction systems can be great additions to constructs that are used to drive high protein expression...also used the hok/sok toxin-antitoxin system to construct a stable synthetic gene circuit that can be used...

...receive an aliquot from the original library constructed by the depositing lab. (For our more popular ...library without an amplification protocol, or have constructed your own library, you’ll need to develop your...protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction, screening...

... prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient bacterial strains,...you’ve got the knowledge to be able to design a construct for the successful and specific expression of ...

... states in deep tissue, in vivo. Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven... (AAV1) - and you can also find several other constructs in plasmid form. Fig. 1: Summary of the...