We narrowed to 202 results for: cycs

...Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in the Periaqueductal...Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. ...

...extracellular destination. At the end of their life cycle, proteins may be transported through the vesicles...proteins it is also possible to follow the life cycle of viruses and intracellular bacteria – have a look...

... to increase yield. The collection process is a cycle of collecting the media and adding fresh media every...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...

... target cells and can be altered by freeze/thaw cycles (Lock et al., 2010). It is recommended that you...are quite stable and will withstand freeze/thaw cycles and dehydration (Howard & Harvey, 2017). You can...

... the destination construct. Reactions are often cycled between 37°C and 16°C to provide optimal reaction...terminal soak after either 37 ºC incubations or cycling.  *Assembly reaction volumes were 20 μl (1, 12 ...

..., one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression...later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used ...

...few things you can try: Increase the number of cycles. After additional rounds of amplification, your...positive and negative droplets. No more than 50 cycles is recommended. Decrease your ramp rate. A rate...

...is tightly coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. ...copy number of pSC101 through a negative feedback cycle. As with DnaA, high concentrations of RepA negatively...

...generally aliquoted to reduce the number of freeze-thaw cycles needed when using the virus. A small aliquot is...lentivirus. Since viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests...

...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

...observed in humans. Thus, understanding their life cycle and processes with their regenerative abilities ...observing and manipulating the entire developmental cycle. A majority of studies involving axolotls focus ...

...grow and maintain in the lab, have a short life cycle of 8 to 14 days, and produce a large number of offspring...small, transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans...

...the most common approaches is to use sequential cycles of primary and secondary antibody labeling and ...secondary, image again, inactivate, and repeat these cycles for all your targets. Of course, you’ll need to...

...plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course, one cannot truly predict the perfect...stable than other viruses, multiple freeze thaw cycles can still reduce AAV titer. For long term storage...

...drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available...

...endosomal trafficking to signaling pathways to the cell cycle. Good tools for measuring intracellular pH are required...movement of two or more targeted loci during the cell cycle or cell differentiation. This tool should delight...visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem cells and a series...

... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...presence of the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the...

...and rtTA. The cells were pulsed with 2µg/ml of Doxycycline for 2 hours, and imaged on the Keyence BZ-9000...dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation. Chemical...

...Spagehetti Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell...

... genetics research, for decades. Its short life cycle, reasonably close genetic homology to humans, and...