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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 267 results for: gnal

Showing: 61 - 80 of 267 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...disease progression. One of the major challenges in signaling research is the inability to replicate the spatio-temporal...spatio-temporal precision with which signaling events occur in a physiological setting. Genetic techniques...typically rely on overexpression or knock-down, where signaling dynamics are influenced by the relatively long...approach, they designed Opto-RTKs, which activate signaling cascades on exposure to low-intensity blue light...intracellular kinase domains into contact and initiating signaling. Grusch et al. demonstrate that Opto-RTKs have...research into its many roles in protein function and signaling networks continues to expand. The labs of Jesse...precisely and non-invasively control neuronal signaling. For more information on DREADDs, please see...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...channels used to control activities like neuronal signaling — think of a mouse with an LED brain implant or...movement, and more, often driven by Rho family signaling pathways. Rho guanine-nucleotide exchange factors...used SuperPLD to manipulate phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to...Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox. ACS Synthetic Biology, 11(1), 515–521....2022.09.010.  Ridley, A. J. (2016). Rho GTPase signalling in cell migration. Current Opinion in Cell Biology...

  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...dyes can result in varying cytoplasmic background signals when used with the same protein (Hayashi-Takanaka... dyes can influence receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also... properties ensure a more consistent and stable signal over extended periods, which is particularly beneficial...crosstalk/crossover to reduce ‘muddying’ of the signal and facilitate clear differentiation between multiple... enable detection of finer details by improving signal-to-noise ratios. Certain dyes have been specifically...

  5. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...may improve the resolution, although background signal would be a concern. Another caveat of the study...protein molecules is necessary for a detectable signal, limiting the sensitivity of the technique. One...molecules (and also available from Addgene!) Since the signal level provided by SunTag is so high, cells may ...publication at Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...proteins play in cell signaling. Understanding how molecules in the Ras signaling pathway interact will...Ras proteins are small GTPases involved in cell signaling pathways that control many cellular processes ...containing genes from a crowd-sourced map of the RAS signaling pathway and includes 180 unique transcripts. Each...Proteins rarely act alone. Molecular processes from signal transduction to transport rely on protein-protein...actuator’s subcellular localization using trafficking signals and named it Magneto 2.0. Using Magneto2.0-p2A-...developing selective kinase inhibitors to probe these signaling pathways or develop potential therapeutics, but...

  7. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...biosensors . Thus, you can map what regions are signaling to a particular site while also activating or ...inhibiting gene expression, detecting neuronal signaling, or manipulating any other genetically controlled...example, say I wanted to access all the neurons that signaled from the hippocampus to Broca’s area. This could...limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would be ...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...on the target protein they tend to have a strong signal and to work in a variety of applications. The desired... to a monoclonal primary antibody (A). To boost signal, consider using subclass specific secondary antibodies...primary/secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you ... have been cross-adsorbed to reduce off-target signals. Use isotype- or subtype-specific secondary antibodies...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...cross-talk is high and your background signal may be higher than the signal generated by the energy transfer...complementary pair of fluorophores. To maximize the FRET signal you should choose the highest quantum yield donor...excited by a specific wavelength of light and the signal is collected by using emission filters chosen for...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ... leading to the emission of photons. Its robust signal, sensitivity, wide dynamic range, and resistance...substrate Akalumine with significantly brighter signal and emission in the near-infrared range, ideal ...bioluminescent chemo-optogenetic control of neuronal signaling. TRUPATH Kit , ONE-GO Biosensors Kit , and G-CASE...AIMTOR : BRET biosensor for live imaging of mTOR signaling. Do you have suggestions for other plasmids that...

  11. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...essential cell surface receptors involved in complex signaling pathways and the linkage of intra- and extracellular...processes, including cell adhesion, migration, and signaling, as well as immune response, tissue repair, and...new tools to identify integrin presence, study signaling mechanisms, or ascertain which integrins are responsible...adhesion and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical...

  12. Chemogenetics Plasmids

    Type
    Collection
    ...relationship between neuronal signaling and behavior. Depending on the signaling pathway the engineered receptor...small molecules, allowing a scientist to control signaling within cells expressing these engineered receptors...

  13. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...bind to intracellular proteins. The fluorescence signal is detected by the flow cytometer and functions... as a proxy for protein expression. Because the signal comes from the antibody-fluorophore conjugate, ...cytometry is the ability to use multiple fluorescent signals (up to a dozen or more!) to measure the relative...applications are typically used for investigating signaling pathways or developing therapeutics. Experimentally...

  14. Synthetic Biology - Overview

    Type
    Collection
    ...networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal...

  15. Deep Dive: qPCR

    Type
    Blog Post
    ...you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence ...double-stranded DNA, when temperatures are high, signal intensity should be low, reflecting the presence...errors and allow for well-to-well normalization of signal. They are often included in commercial sets and...

  16. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...the expression level necessary to observe a signal. Red signal intensity increases with GFP concentration...For low-abundance GFP fusions, a photoconversion signal may not be observed. Sattarzadeh et al. found that...

  17. CRISPR Guide

    Type
    Collection
    ...PAM) The PAM sequence (NGG) serves as a binding signal for Cas9, but the exact sequence depends on which...the aptamer improves stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius...degraded by Cas12a, it will release a quantifiable signal that indicates the presence of your target DNA ...cleaves the reporter as well, and a quantifiable signal is produced, indicating the presence of that specific...Pederson, T. (2018). CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging. Nature Methodss...Vale, R. D. (2014). A Protein-Tagging system for signal amplification in gene expression and fluorescence...

  19. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ... increased brightness, they also found that the signal to noise ratio can be increased from 2-fold (by.... Of these, 26 of the 46 loci gave a detectable signal in human U2OS cells. Based upon this, they chose...Hanhui et al. “CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.” Nature Methods...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...assays have lower background levels and improved signal sensitivity. Another advantage: luciferase reporters... Cofactors/substrates Required Not required Signal strength Lower Higher Sensitivity Higher Lower... similar approach can be used to analyze cell signaling pathways or miRNA knockdown. BRET for the best...
Showing: 61 - 80 of 267 results