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  2. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...PubMed Preferences” and click on the “Filters & Icons” link (see image to the right). Once on the “Filters...” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...

  3. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ... bottom right corner of each page there’s a blue icon displaying the word “Help” next to an encircled ...wherever you are on our site. Simply click on the icon, search for answers, and choose a result. By clicking...

  4. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... of this system is the single-color labeling; multicolor labeling would enable the visualization of multiple...excited to see what’s coming next! References Multicolor CRISPR labeling of chromosomal loci in human ...

  5. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...10;4:2153. doi: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared...

  8. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...like any other youngster. His parents, Kristi and Rico, had to keep him from running too much as any jarring...which people dress up like superheroes or other iconic characters and run a 5K around Cambridge. We will...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  12. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...fluorescent proteins Commonly used filter sets for multicolor imaging include ones designed for CFP, YFP, and...compartments. I hope this sheds some light on multicolor imaging with fluorescent proteins. With the right...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    ...are receptors for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Streptomyces , and others) Drosophila Plants (monocots and dicots) C. elegans Yeast ( S. cerevisiae and S. pombe ...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal loci in human ...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...is critical that the deletion and non-deletion amplicons be easily resolved on an agarose gel for multiplexing... with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into a plasmid...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (...

  19. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...the quantity of DNA after a PCR reaction as the amplicon doubles every reaction cycle. However, it is common...goal is to amplify a specific target of DNA. This amplicon can be used in molecular cloning to create a plasmid...
Showing: 61 - 80 of 205 results