Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 82 results for: lentiviral construct

Showing: 61 - 80 of 82 results

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    ...was rapidly able to support sharing of CRISPR constructs and information across the globe.  “Read the ...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing...

  3. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...cell types, while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing...most often expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs...C. (2006). Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnology,...

  5. CRISPR References and Information

    Type
    Collection
    ...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new... Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection and ...triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal...

  6. Neurodegeneration Research Collection

    Type
    Collection
    ...Synuclein, and more. New and Noteworthy: Use tau constructs to study aggregation. Saha et al. Nat Commun.... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...

  7. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...selectable marker. This vector can express your construct in target cells transiently or constitutively ...Some customization is required, but many viral constructs have already been built and are available on ... embryonic stem cells. Viral integration of a construct is also reliably higher than traditional transfection...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  8. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...the design and construction steps are complete, it’s time to make virus! The constructed viral vector containing...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  9. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...

  10. Important Considerations When Using AAVs

    Type
    Blog Post
    ...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted...different binding affinities or negative-control constructs used to characterize the sensor's behavior in...biosensor in a novel context or modifying an existing construct, as fluorescence properties can be affected by...fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...for popular biosensors in different expression constructs? Browse the table below or search for a biosensor...

  12. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral... 293T (HEK293T) cells (Figure 3A), similar to lentiviral packaging methods . The three plasmids described...BioRender.com. Viral Vector Integration Much like lentiviral vectors, gamma-retroviral vectors integrate at...to make stable cell lines. Like their popular lentiviral counterparts, gamma-retroviral vectors offer ...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...cell line protocol . Addgene’s protocol is for lentiviral vectors, but a similar method can be applied ...

  13. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . In...different generations. Lentiviral Generations First-generation First-generation lentiviral plasmids are largely...Second-generation Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...that particles are not sprayed into the air. For lentivirus and other BSL-2 materials, you’ll need to use...for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge bin covers, which...

  15. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...justifying each single element used to build the construct and explain why they selected a certain target...has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...

  16. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...

  17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...however, suffer from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome...be directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...

  18. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...terminator may be desired for multi-cistronic constructs where high termination efficiency is necessary... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

  19. Molecular Biology Reference

    Type
    Guide
    ...interested in. Self-replicating - Once you have constructed a plasmid, you can easily make an endless number...these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...

  20. CRISPR Guide

    Type
    Guide
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous... the HDR pathway can also help. Additionally, constructs with Cas9 fused to proteins that modulate HDR...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...
Showing: 61 - 80 of 82 results